1992 Fiscal Year Annual Research Report

液胞型ATP分解酵素の細胞機能

Research Project

Project/Area Number	04266103
Research Institution	Tokyo Institute of Technology
Principal Investigator	吉田賢右東京工業大学, 資源化学研究所, 教授 (90049073)
Co-Investigator(Kenkyū-buntansha)	柿沼喜己千葉大学, 薬学部, 助手 (80134394) 前田正知大阪大学, 産業科学研究所, 助教授 (80190297) 田代裕関西医科大学, 医学部, 教授 (40077558) 向畑恭男名古屋大学, 理学部, 教授 (10028110) 安楽泰宏東京大学, 理学部, 教授 (20012643)
Keywords	液胞型ATP分解酵素 / H^+輸送 / イオンポンプ / 胃酸分泌酵素 / バフィロマイシン / 古細菌 / V-ATPase / 液胞膜
Research Abstract	平成4年度には以下のような進展があった。 1.培養細胞において、液胞型ATP分解酵素の阻害剤バフィロマイシンの効果が調ベられた。バフィロマイシンはプロラクチンの細胞内輸送をトランスゴルジ綱様体ないし、分泌顆粒の成熟過程で阻害した。またバフィロマイシンは細胞の増殖を抑制し、S期の細胞を増加させることが明らかとなった。 2.液胞型ATP分解酵素のサブユニットBは腎臓型と普遍型の2種存在しており、両種ともMDBK細胞で発現していることが明らかとなった。 3.酵母液胞型ATP分解酵素の活性発現に必須な遺伝子VMA12の構造が決定された。本酵素のプロティン・スプライシングは、(1)Cys738における切断(2)Cys284における切断(3)新しく生じたC端とN端の再結合という3段階を経ることが明らかとなった。 4.古細菌には液胞型ATP分解酵素しか存在していないものと思われたが、一部のメタン菌にはF型ATP分解酵素が存在していることがわかった。 5.好塩性古細菌ハロバクテリアの液胞型ATP分解酵素の触媒頭部は硝酸イオンによって阻害されるが、本来の活性であるATP合成活性は同イオンによっては阻害されないことが判明した。 6.連鎖球菌のNa^+輸送性、液胞型ATP分解酵素のクローニングが進行した。

Research Products
(18 results)

All Other

All Publications (18 results)

[Publications] Y.Anraku: "Geneticand cell biological aspects of the yeast vacuolar H^+-ATPase." J.Bioenerg.Biomembr.24. 395-405 (1992)
[Publications] Y.Anraku: "Molecular genetics of the yeast vacular H^+-ATPase" J.Exp.Biol.172. 67-81 (1992)
[Publications] Y.Wada: "Genes for directing vacuolar morphogenesis in Saccharomyces cerevisiae.1.Isolation and characterization of two classes of vam mutants." J.Biol.Chem.267. 18665-18670 (1992)
[Publications] Y.Wada: "Gemes for directing vacuolar morphogenesis in Saccharomyces cerevisiae.2.VAM 7,a gene for regulating morphogenic assembly of the vacuoles." J.Biol.Chem.267. 18671-18675 (1992)
[Publications] Y.Wada: "Chloride transport of yeast vacuolar membrane vesicles;A study of in vitro vacuolar acidification" Biochim.Biophys.Acta. 1101. 296-302 (1992)
[Publications] R.Hirata: "Mutations at the putative junction sites of the yeast VMA1 protein,the catalytic subunit of the vaduolar membrane H^+-ATPase,inhibit its processing by protein splicing." Biochem.Biophys.Res.Commun.188. 40-47 (1992)
[Publications] Y.Mukohata: "Photophosphorylation elements in Halobacteria-an A-type ATP synthase and bacterial rhodopsins." J.Bioenerg.Biomembr.24(6). 547-553 (1992)
[Publications] K.Ihara: "Halobacterial A-ATP synthase in relation to V-ATPase" J.exp.Biol.172. 475-485 (1992)
[Publications] T.Takada: "Quantitative immunogold localization of Na,K-ATPase along rat nephron." Histochemistry. 98. 183-197 (1992)
[Publications] K.Kawasaki: "Quantitative immunoelectron microscopic localization of Na,K-ATPase α-subunit in the epithelial cells of rat vestibular apparatus." Hear.Res.60. 64-72 (1992)
[Publications] S.Noguchi: "Assembly of a hybrid from the α subunit of Na^+/K^+- ATPase and the β subunit of H^+/K^+- ATPase." Biochem.Biophys.Res.Commun.182. 659-666 (1992)
[Publications] M.Hasebe: "Vacuolar type H^+- ATPase genes:presence of four genes including pseudogenes for the 16-kDa proteolipid subunit in the human genome." Biochem.Biophys.Res.Commun.183. 856-863 (1992)
[Publications] S.Tamura: "Sequence motif in control regions of the H^+/K^+ATPase α and β subunit genes recognized by gastric spectific nuclear protein(s)." FEBS Lett.298. 137-141 (1992)
[Publications] Y.Moriyama: "Involvement of a non-proton pump factor (possibly Donnan-type equilibrium)in maintenance of an acidic pH in lyssomes." FEBS Lett.302. 18-20 (1992)
[Publications] T.Hisabori: "Single Site Hydrolysis of TNP-ATP by F_1-ATPase from the Thermophilic Bacterium PS3 Is Accelerated by the Chase-Addition of Excess ATP" J.Biol.Chem. 267. 4551-4556 (1992)
[Publications] K.Tozawa: "Molecular Dissection of the β subunit of F_1-ATPase into Peptide Fragments" J.Biol.Chem. 267. 16484-16490 (1992)
[Publications] M.Sumi: "A DNA Fragment Homologous to F_1-ATPase α Subunit Was Amplified from Genomic DNA of Methanosarsina barker:Indication of an Archaebacterial F-type ATPase" FEBS Lett.314. 207-210 (1992)
[Publications] N.Ishii: "Three Dimensional Structure of F_1-ATPase of Thermophilic Bacterium PS3 Obtained from Electrom Crytallography" J.Biochem.113. 245-250 (1993)

1992 Fiscal Year Annual Research Report

液胞型ATP分解酵素の細胞機能

Principal Investigator

吉田 賢右 東京工業大学, 資源化学研究所, 教授 (90049073)

Research Products

[Publications] Y.Anraku: "Geneticand cell biological aspects of the yeast vacuolar H^+-ATPase." J.Bioenerg.Biomembr.24. 395-405 (1992)

[Publications] Y.Anraku: "Molecular genetics of the yeast vacular H^+-ATPase" J.Exp.Biol.172. 67-81 (1992)

[Publications] Y.Wada: "Genes for directing vacuolar morphogenesis in Saccharomyces cerevisiae.1.Isolation and characterization of two classes of vam mutants." J.Biol.Chem.267. 18665-18670 (1992)

[Publications] Y.Wada: "Gemes for directing vacuolar morphogenesis in Saccharomyces cerevisiae.2.VAM 7,a gene for regulating morphogenic assembly of the vacuoles." J.Biol.Chem.267. 18671-18675 (1992)

[Publications] Y.Wada: "Chloride transport of yeast vacuolar membrane vesicles;A study of in vitro vacuolar acidification" Biochim.Biophys.Acta. 1101. 296-302 (1992)

[Publications] R.Hirata: "Mutations at the putative junction sites of the yeast VMA1 protein,the catalytic subunit of the vaduolar membrane H^+-ATPase,inhibit its processing by protein splicing." Biochem.Biophys.Res.Commun.188. 40-47 (1992)

[Publications] Y.Mukohata: "Photophosphorylation elements in Halobacteria-an A-type ATP synthase and bacterial rhodopsins." J.Bioenerg.Biomembr.24(6). 547-553 (1992)

[Publications] K.Ihara: "Halobacterial A-ATP synthase in relation to V-ATPase" J.exp.Biol.172. 475-485 (1992)

[Publications] T.Takada: "Quantitative immunogold localization of Na,K-ATPase along rat nephron." Histochemistry. 98. 183-197 (1992)

[Publications] K.Kawasaki: "Quantitative immunoelectron microscopic localization of Na,K-ATPase α-subunit in the epithelial cells of rat vestibular apparatus." Hear.Res.60. 64-72 (1992)

[Publications] S.Noguchi: "Assembly of a hybrid from the α subunit of Na^+/K^+- ATPase and the β subunit of H^+/K^+- ATPase." Biochem.Biophys.Res.Commun.182. 659-666 (1992)

[Publications] M.Hasebe: "Vacuolar type H^+- ATPase genes:presence of four genes including pseudogenes for the 16-kDa proteolipid subunit in the human genome." Biochem.Biophys.Res.Commun.183. 856-863 (1992)

[Publications] S.Tamura: "Sequence motif in control regions of the H^+/K^+ATPase α and β subunit genes recognized by gastric spectific nuclear protein(s)." FEBS Lett.298. 137-141 (1992)

[Publications] Y.Moriyama: "Involvement of a non-proton pump factor (possibly Donnan-type equilibrium)in maintenance of an acidic pH in lyssomes." FEBS Lett.302. 18-20 (1992)

[Publications] T.Hisabori: "Single Site Hydrolysis of TNP-ATP by F_1-ATPase from the Thermophilic Bacterium PS3 Is Accelerated by the Chase-Addition of Excess ATP" J.Biol.Chem. 267. 4551-4556 (1992)

[Publications] K.Tozawa: "Molecular Dissection of the β subunit of F_1-ATPase into Peptide Fragments" J.Biol.Chem. 267. 16484-16490 (1992)

[Publications] M.Sumi: "A DNA Fragment Homologous to F_1-ATPase α Subunit Was Amplified from Genomic DNA of Methanosarsina barker:Indication of an Archaebacterial F-type ATPase" FEBS Lett.314. 207-210 (1992)

[Publications] N.Ishii: "Three Dimensional Structure of F_1-ATPase of Thermophilic Bacterium PS3 Obtained from Electrom Crytallography" J.Biochem.113. 245-250 (1993)

吉田賢右東京工業大学, 資源化学研究所, 教授 (90049073)