1994 Fiscal Year Annual Research Report

ATP合成酵素の高次構造

Research Project

Project/Area Number	04266104
Research Institution	Osaka University
Principal Investigator	二井將光大阪大学, 産業科学研究所, 教授 (50012646)
Co-Investigator(Kenkyū-buntansha)	太田成男日本医科大学, 老人病研究所, 教授 (00125832) 白木原康雄兵庫教育大学, 学校教育学部, 助教授 (20150287) 伊藤雄而山梨大学, 教育学部, 教授 (10013517) 小沢高將名古屋大学, 医学部, 教授 (80022771) 田川邦夫岡山県立大学, 保健福祉学部, 教授 (40028296)
Keywords	ATP合成酵素
Research Abstract	ATPは、ミトコンドリアおよび葉緑体においていずれも、電子伝達鎖の形成するH^+の電気化学的ポテンシャル差を駆動力とし、ATP合成酵素(F_0F_1)によって作られている。また、ATP合成酵素の生化学的実体と性状は、主に研究代表者らによって実証されてきた。既に全一次構造が明らかにされており、ATP結合部位、H^+輸送路等の基本的な機能部位の構造が明かにされようとしている。したがって現時点では本酵素の反応機構と高次構造を統合的に理解し、決定的に実証しなければならない段階となっている。このような現状をふまえ、ATP合成酵素の高次構造を明らかにし、反応機構を解明することを本研究の究極の目的とした。 ATP合成酵素(F_0F_1)の各サブユニットのレベルで高次構造と反応機構に関して以下の主な成果を得た。a)ミトコンドリアF_0F_1の分子構築とH^+輸送に重要なアミノ酸残基を確立した。b)2.5Å分解能の回折パターンを与えている好熱菌F_1(α3β3複合体)の結晶を用い高次構造のモデルを得た。c)F_0およびF_0F_1を大腸菌において大量に作らせ2次元結晶化の条件を決め、F_0を膜中に再構成し、H^+輸送に伴う動的構造を推定する実験系を確立した。d)βサブユニット変異(とその抑圧変異)を導入し、活性中心とその活性中心近傍の構造を推定した。βサブユニットのGlu181、Arg182、Lys-155、Thr-156が触媒残基であることを立証した。e)好熱菌の再構成した二つのATPase複合体α1β1とα3β3の構造維持機構を明かにした。f)ミトコンドリアの調節蛋白とF_1F_0の相互作用を明かにした。g)Enterococcus hiraeのF_0F_1の発現機構/構造/機能を他の生物のものとを比較した。それぞれの成果は担当の研究者を中心に他の研究者の密接な協力によって得られた。

Research Products

(81 results)

All Other

All Publications (81 results)

[Publications] T.Manabe,T.Yoshimori,Y.Tashiro,M.Futai,et al.: "ATP-dependent uptake anti-neoplastic agents by acidic organelles." J.Biochem.115. 213-218 (1994)
[Publications] H.Omote,M.-Y.Park,M.Maeda and M.Futai: "The α/β subunit interaction in H^+-ATPase(ATP synthase):an Escherichia coil α subunit mutation (αArg-296→Cys)restored coupling efficiency to the deleterious β subunit mutant(βSer-174→Phe)." J.Biol.Chem.269. 10265-10269 (1994)
[Publications] Y.Etani,S.Shimada,M.Hasebe,M.Futai,M.Maeda: "Genes of Members of the GATA-binding protein family(GATA-GT1 and GATA-GT2)together with H^+K^+-ATPase are specifically transcribed in gastric parietal cells." FEBS Lett.340. 117-120 (1994)
[Publications] M.Futai,A.Iwamoto,H.Omote,M.-Y.Park,M.Maeda: "Catalysis and energy coupling of H^+-ATPase(ATP Synthase):Molecular biological approaches." Biochimica et Biophysica Acta. 1187. 165-170 (1994)
[Publications] M.-Y.Park,H.Omote,M.Maeda,M.Futai: "Conserved Glu-181 and Arg-182 residues of Escherichia coli H^+-ATPase(ATP synthase)β subunit are essential for catalysis:Properties of 33 mutants between βGlu-161 and βLys-201 residues." J.Biochem.116. 1139-1145 (1994)
[Publications] H.Mori,T.Fukui,Y.Moriyama,M.Futai,Y.Tashiro: "Association of N-ethylmaleimide-sensitive factor with synaptic vesicles." FEBS Lett.350. 253-257 (1994)
[Publications] A.M.V.Dunean,T.Ozawa,H.Suzuki and R.Rosen: "Assignment of the gene for the cytochrome cl subunnit of the mitochondrial cytochrome bcl complex(CYCl)to human chromosome 8q24.3." Genomics. 19. 400-401 (1994)
[Publications] M.M.Islam,M.Tanaka,K.Torii,N.Hattori,T.Ozawa: "A complete cDNA sequence for core I protein subunit of human ubiquinol-cytochrome c reductase." Biochem.Mol.Biol.Int. 32. 797-805 (1994)
[Publications] K.Katsumata,M.Hayakawa,S.Sugiyama,T.Ozawa,et all.: "Fragmentation of human heart mitochondrial DNA associated with premature aging." Biochim.Biophys.Res.Commun.202. 102-110 (1994)
[Publications] A.Duncan.L.Anderson.C.duff,T.Ozawa,H.Suzuki and et all.: "Assignment of the gene(UQCRF51)for the Rieske iron-sulfur protein subunit of the mitochondrial cytochrome bcl complex to the 22q13 and 19q12-q13.1 regions of the human genome." Genomics. 21. 281-283 (1994)
[Publications] M.Tanaka and T.Ozawa: "Strand asymmetry in human mitochondrial DNA mutations." Genomics. 22. 327-335 (1994)
[Publications] W.Sato,M.Tanaka,S.Sugiyama,G.Takada,T.Ozawa and et all: "Cardiomyopathy and angiopathy in patients with mitochondrial myopathy,lactic acidosis and stroke-like episodes." Am.Heart J.128. 733-741 (1994)
[Publications] A.Iwamoto,Y.Orita,M.Maeda,and M.Futai: "N-Ethylmaleimide-sensitive mutant(βVal-153→Cys)Escherichia coli F1 ATPase:Cross-Linking of the mutant β subunit with the α subunit." FEBS Lett.352. 243-246 (1994)
[Publications] A.Kovalenko,M.Tanaka,W.Borgeld and T.Ozawa: "A common hot spot for somatic and germline mutations in rat mitochondrial DNA:Analysis by fluorescence-based sscp." Biochem.Mol.Biol.Int.34. 1205-1214 (1994)
[Publications] T.Furui,O.Kurauchi,T.Ozawa,Y.Tomoda,et all.: "Decrease in cytochrome c oxidase and cytochrome oxidase subunit I messenger ribonucleic acid levels in preeclamptic pregnancies." Obstet.Gynecol.84. 283-288 (1994)
[Publications] S.Ikebe,M.Tanaka,and T.Ozawa: "Point mutations of mitochondrial genome in parkinson's disease." Mol.Brain.Res.(in press).
[Publications] S.A.Kovalenco,M.Tanaka and T.Ozawa: "Simple methods for preparation of plasmid DNA yielding long and accurate sequence data." Nucleic.Acids.Res.(in press).
[Publications] T.Ozawa,K.Katsumata,M.Sekiguchi and et all.: "Genotype and phenotype of a severe mitochondrial cardiomyopathy:a recipient of heart transplantation and the genetic control." Biochim.Biophys.Res.Commun.(accepted).
[Publications] 伊藤雄而: "中性子小角散乱装置" 蛋白質・核酸・酵素. 39(11). 2026-2032 (1994)
[Publications] T.Uyama,Y.Moriyama,M.Futai and H.Michibata.: "Immunological detection of a vacuolar-type H^+-ATPase in vanadocytes of the ascidian ascidia sydneiensis samea." J.of Experi.Zool.207. 148-154 (1994)
[Publications] Y.Ito,M.Imai,Y.Moriyama and M.Futai: "Solution structure of F_0F_1-ATPase.Utilisation des neutrons et du rayonnement synchrotron en biologie:diffusion centrale,duffusion duffuse et diffusion inelastique." St Nizier du Moucherotte,France. Nov.21-25 (1994)
[Publications] 伊藤雄而、佐藤衛、原田三男、太田成男、香川靖雄: "TF1のHexamer-Dimer解離・会合反応とBinding change(Rotational catalysis)mechanism." 文部省科学研究非重点領域研究「細胞エネルギー共役系の構築と調節」平成6年度研究成果報告書. 28-29 (1994)
[Publications] Y.Moriyama,V.Patel,M.Futai: "Quinnacrine mustard and lipophilic entions inhibitory to both vacuolar H^+-ATPase and F_0F1-ATP synthase" FEBS Lett.359. 69-72 (1995)
[Publications] S.Ueno,K.Takeda,M.Maeda,M.Futai,M.Kawamura.: "Functional consequances of the substitution of disulfide-bonded segment,Cys127-Cys150,located in the extracellular domain of the Na,K-ATPase β subunit:Arg148 is essential for the functional expression of Na,K-ATPase." (in press).
[Publications] I.Hayashi,S.Ohta,Y.Kagawa,H.Kondo,H.Kaneda,et all: "Nuclear but not mitochondrial genome involvement in human age-related mitochondrial dysfunction." J.Biol.Chem.269(9). 6878-6883 (1994)
[Publications] T.Koike,N.Inohara,I.Sato,Y.Kagawa,S.Ohta,et all.: "Transcription of the MRP RNA gene in frog stage I oocytes requires a novel ciselement." Novel Biochim.Biophys.Res.Comun.202(1). 225-233 (1994)
[Publications] T.Odagiri,K.Tominaga,K.Tobita and S.Ohta: "An amino acid change in the non-structural NS2 protein of an influenza A virus mutant is responsible for the generation of defective interfering(DI)particles by amplifying DI RNAs and supressing complementary RNA synthesis." J.General Viol.75. 43-53 (1994)
[Publications] S.Akiyama,H.Endo,N.Inohara,S.Ohta and Y.Kagawa: "Gene structure and cell type-specific expression of the human ATP synthase a subunit." Biochim.Biophys.Acta.1219. 129-140 (1994)
[Publications] S.Ohta,Y.Kagawa,M.Yoshida,M.Nishizawa,et all.: "Widespread tissue distribution of multiple mitochondrial DNA deletions in familial mitochondrial myopathy." Muscle and Nerve. 17. 741-746 (1994)
[Publications] J-I.Hayashi,S.Ohta,Y.Kagawa,D.Takai,S.Miyabayashi,K.Tada: "Functional and morphological abnormalities of mitochondria in human cells containing mitochondrial DNA with pathogenic point mutations in tRNA genes." J.Biol.Chem.269(29). 19060-19066 (1994)
[Publications] K.Nakano,C.Takase,S.Nakagawa,J.Inazawa,S.Ohta: "Isolation characterization and structural organization of the gene and pseudogene for the dihydrolipoamide succinyltransferase component of the human 2-oxoglutarate dehydrogenase complex." Eur.J.Biochem.224. 179-189 (1994)
[Publications] M.Harada,H.Kihara,H.Tsuruta,S.Ohta and Y.Kagawa: "An ATP-hydrolyzing excited state of the reconstituted a3b3 complex of ATP synthase from thermophilic bacterium PS3:the structural feature revealed by time-resolved small-angle scattering with synchrotron radiation." J.Biochem.(in press).
[Publications] 白岩伸子、太田成男: "ミトコンドリア脳筋症" 現代化学増刊23 遺伝子診断と遺伝子治療. 49-54 (1994)
[Publications] 太田成男: "ミトコンドリアDNAの変異によって生じるミトコンドリア病とミトコンドリアの形成制御機構" 日本生化学学会誌生化学. (印刷中).
[Publications] 太田成男: "ミトコンドリア病の遺伝子治療" 臨床科学. 30(4). 464-468 (1994)
[Publications] Y.Orii,T.Mogi,M.Sato,T.Hirano and Y.Anraku: "Facilitated intramolecular electron transfer in the Escherichia coli bo-type ubiquinol oxidase requires chloride." Biochemistry.(in press).
[Publications] T.Mogi,K.Saiki and Y.Anraku,et all.: "Biosynthesis and functional role of heme O and heme A." Mol.Microbiol.14. 391-398 (1994)
[Publications] T.Mogi,H.Nakamura,Y.Anraku,et all.: "Molecular structure of a heme-copper redox center of the Escherichia coli ubiquinol oxidase:Evidence and model." J.Biochem.116. 741-747 (1994)
[Publications] T.Mogi,K.Matsushita,O.Adachi and Y.Anraku,et all.: "Structure-function studies on the ubiquinol oxidation site of the cytochrome bo complex from Escherichia coli using p-benzoquinones and substituted phenols." J.Biol.Chem.269. 28899-28907 (1994)
[Publications] M.Sato,T.Mogi,T.Kitagawa,H.Iwamura,Y.Anraku,et all.: "Identification of a novel quinone binding site in the cytochrome bo complex from Escherichia coli." J.Biol.Chem.269. 28908-28912 (1994)
[Publications] Y.Moriyama,Y.Tashiro,M.Takahashi,M.Maeda,M.Futai: "Microvesicles isolated from bovine posterior pituitary accumulate norepinephrine." (in press).
[Publications] K.Saiki,T.Mogi,M.Kawasaki and Y.Anraku: "An Escherichia coli cyoE gene homologue in thermophilic Bacillus PS3 encodes a thermotolerant heme O synthase." FEBS Lett.351. 385-388 (1994)
[Publications] S.Hirota,T.Mogi,T.Ogura,Y.Anraku,T.Kitagawa,et all.: "Observation of the Fe-O2 and Fe^<1V> =O Raman bands in dioxygen reduction by cytochrome bo-type ubiquinol oxidase from Escherichia coli." FEBS Lett.352. 67-70 (1994)
[Publications] Y.Orii,T.Mogi,M.Kawasaki,Y.Anraku: "Facilitated intra-molecular electron transfer in the cytochrome bo-type ubiquinol oxidase initiated upon reaction of the fully-reduced enzyme with dioxygen." FEBS Lett.352. 151-154 (1994)
[Publications] T.Uno,T.Mogi,Tsubaki,Y.Nishimura and Y.Anraku: "Resonance Raman and Fourier-transform infrared studies on the subunit I histidine mutants of the cytochrome bo complex in Escherichia coli:Molecular structure of redox centers." J.Biol.Chem.269. 11912-11920 (1994)
[Publications] 茂木立志: "大腸菌チトクロムbo型ユビキノール酸化酵素の構造と分子生理" 生化学. 67(2)(印刷中).
[Publications] T.Uyama,Y.Moriyama,M.Futai and H.Michibata: "Immunological detection of a vacuolar-type H^+-ATPase in vanadocytes of the ascidian Ascidia sydneiensis samea." J.Exp.Zool. 270. 148-154 (1994)
[Publications] K.Kanamori and H.Michibata: "Raman specroscopic study of the vanadium and sulfate in blood cell homogenates of the ascidian,Ascidia gemmata." J.Mar.Biol.Ass.U.K.74. 279-286 (1994)
[Publications] I.Inoue and T.Higuti: "ATP-sensitive K^+-channel in the mitochondrial inner membrane in:handbook of molecular and cellular physiology of membrane channels." Ed.Peracchia,C.(Invited review)Academic Press. 549-554 (1994)
[Publications] 道端斉: "原索動物ホヤによるバナジウムの還元濃縮機構" 生化学. 66. 495-509 (1994)
[Publications] K.Mimura,M.Tsujimoto,H.Arai,K.Inoue,et all.: "Chinese hamster ovary cells expressing a novel type of acetylated LDL receptor." J.Biol.Chem. In press.
[Publications] Y.Sato,K.Nishikawa,H.Arai,K.Murofushi,K.Inoue,et all.: "Side chain structure is critical for transport of sterols from lysosomes to cytoplasm." Biochem.Biophys.Acta. in press.
[Publications] K.Aikawa,Y.Fujimoto,H.Arai and K.Inoue,et all.: "Structure-specific inhibition of lysosomal cholesterol transport in macrophages by various steroids." Biochim.Biophys.Acta.1213. 127-134 (1994)
[Publications] 新井洋由: "コレステロールの細胞内輸送機構" 蛋白質・核酸・酵素. 39(9). 1518-1531 (1994)
[Publications] A.Tohgo,N.Noguchi,Y.Furuya,H.Yonekura,H.Okamoto,et all.: "Essential cysteine residues for cyclic ADP-ribose synthesis and hydrolysis by CD38." J.Biol.Chem.269. 28555-28557 (1994)
[Publications] O.Tanaka,H.Kondo,S.Takasawa,T.Yoshimoto,H.Okamoto,et all.: J.Biol.Chem.269. 21223-21228 (1994)
[Publications] M.Unno,O.Tanaka,A.J.Bone,S.Takasawa,H.Okamoto,et all.: "Pancreatic beta-cell replication and amelioration of surgical diabetes by Reg protein." Proc.Natl.Acad.Sci.U.S.A.91. 3589-3592 (1994)
[Publications] S.-H.Hua,Y.Furuya,M.Nohmi,H.Okamoto,K.Kuba,et all.: "Cyclic ADP-ribose modulates Ca2-release channels for activation by physiological Ca2-entry in bullfrog sympathetic neurons." Neuron. 12. 1073-1079 (1994)
[Publications] H.Higashida,S.Takasawa,H.Okamoto,D.A.Brown,et all.: "Nicotinamide adenine dinucleotide regulates muscarinic receptor-coupled K+(M)channels in rodent NG 108-15 cells." J.Physiol.482. 317-323 (1995)
[Publications] S.Takasawa,A.Tohgo,Y.Furuya,H.Yonekura,H.Okamoto,et all.: "Cloning and characterization of cDNA encoding rat ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase(homologue to human CD38)from islets of langerhans." Biochim.Biophys.Acta.1223. 160-162 (1994)
[Publications] Y.Hirata,Y.Ohsugi,H.Okamoto,T.Kaisho,T.Hirano,et all.: "ADP ribosyl cyclase activity of a novel bone marrow stromal cell surface molecule,BST-1." FEBS Lett.356. 244-248 (1994)
[Publications] A.Berlova,A.Tohgo,T.Karasawa,T.Takeuchi,H.Okamoto,et all.: "Assignment of the CD38 gene encoding human leukocyte antigen cd38(ADP-ribosyl cyclase/Cyclic ADP-ribose hydrolase)to chromosome 4p15." Cytogenet.Cell Genet.69. 38-39 (1995)
[Publications] K.Nakagawara,H.Miyashita,H.Yonekura,H.Okamoto,et all.: "Isolation,structural determination and expression of a novel rag gene,human rag Iβ." Biochim.Biophys.Acta.1217. 199-202 (1994)
[Publications] A.Tohgo,H.Yonekura,N.Hayashi,H.Okamoto,et all.: "Structural determination and characterization of a 40 kDa protein isolated from 40S ribosomal subunit." FEBS Lett.340. 133-138 (1994)
[Publications] T.Watanabe,M.Unno,H.Miyashita,H.Okamoto,et all.: "Structure and expression of a novel rat reg III gene." Gene. 144. 315-316 (1994)
[Publications] H.Yonekura,Y.Suzuki,S.Takasawa,H.Okamoto,et all.: "Characterization of the 5'-regulatory region of the rat Reg I gene." Biochim.Biophys.Acta.1219. 241-243 (1994)
[Publications] A.Nagai,H.Matsumiya,S.Yasui,H.Okamoto,K.Konno,et all.: "Effects of nicotinamide and niacin on bleomycin-induced acute injury and subsequent fibrosis in hamster lungs." Experimental Lung Research.20. 263-281 (1994)
[Publications] T.Numakunai,K.Shikama,S.Takasawa,H.Okamoto,et all.: "The structure of the Aplysia kurodai gene encoding ADP-ribosyl cyclase,a secondmessenger enzyme." Gene.(in press).
[Publications] H.Okamoto: "New aspects to the functioning and regeneration of pancreatic β-cells:Cyclic ADP-ribose and reg gene." Islet Physiology and Pathophysiology,Plenum Press,N.Y.in press.
[Publications] H.Okamoto,S.Takasawa and A.Tohgo: "New aspects to the physiological significance of NAD,poly ADP-ribose and cyclic ADP-ribose." Biochim. (in press).
[Publications] H.Okamoto,S.Takasawa,I.Kato,N.Noguchi and H.Yonekura,et all.: "A role of cyclic ADP-ribose in stimulus-secretion coupling in pancreatic β-cells." Elsevier,Amsterdam. (in press).
[Publications] H.Yonekura,M.Unno,Y.Suzuki,H.Miyashita,H.Okamoto,et all.: "Structure and expression of reg gene family." Smith-Gordon,London. (in press).
[Publications] 岡本宏: "膵β細胞破壊と防止についての岡本モデルとその展開" 糖尿病. 37. 99-102 (1994)
[Publications] 高沢伸、那谷耕司、杉本孝子、武山淳二、米倉秀人、岡本宏他: "膵β細胞機能発現の新しい細胞内情報伝達物質cyclic ADP-ribose" 糖尿病記録号1993. 247-251 (1994)
[Publications] 加藤一郎、鈴木雄、赤羽敦也、米倉秀人、岡本宏、田中理: "膵ランゲルハンス島β細胞でVIP遺伝子を過剰発現するトランスジェニックマウスの作成と導入遺伝子による血糖降下作用" 分子糖尿病学. 5. 73-80 (1994)
[Publications] K.Saika,K.Inaka,T.Matsui,M.Yoshida and K.Miki: "Crystallization of mutant β subunit of F1-ATPase from therinophilic bacillus PS3" J.Mol.Biol.242. 709-711 (1994)
[Publications] 三木邦夫: "タンパク質結晶学と構造生物学の新たな飛躍" 化学. 49(8). 592-593 (1994)
[Publications] J.Ezaki,L.S.Wolfe,T.Higuti,K.Ishidoh,and E.Kominami: "Specific delay of degradation of mitochondrial ATP synthase subunit c in late infantile neuronal ceroid-lipofuscinosis(Batten Disease)." J.Neurochemistrry. 64(2)(in press).
[Publications] T.Tamura,H.Nothwang,T.Higuti,N.Ishida,K.Tanaka: "Isolation and characterization of a-type HC3 and b-Type HC5 subunit genes of human proteasomes:distinct gene organizations and promoter functions" Mol.Cell.Biol.(in press).
[Publications] M.Prescott,T.Higuti,P.Nagley,R.J.Devenish: "The functional expression of A rat cDNA encoding OSCP in the yeast saccharomyces cerevisiae." Biochem.Biophys.Res.Commun.in press).
[Publications] R.K.Nakamoto M.Futai: "Biomembranes V" (in press),

1994 Fiscal Year Annual Research Report

ATP合成酵素の高次構造

Principal Investigator

二井 將光 大阪大学, 産業科学研究所, 教授 (50012646)

Research Products

[Publications] T.Manabe,T.Yoshimori,Y.Tashiro,M.Futai,et al.: "ATP-dependent uptake anti-neoplastic agents by acidic organelles." J.Biochem.115. 213-218 (1994)

[Publications] H.Omote,M.-Y.Park,M.Maeda and M.Futai: "The α/β subunit interaction in H^+-ATPase(ATP synthase):an Escherichia coil α subunit mutation (αArg-296→Cys)restored coupling efficiency to the deleterious β subunit mutant(βSer-174→Phe)." J.Biol.Chem.269. 10265-10269 (1994)

[Publications] Y.Etani,S.Shimada,M.Hasebe,M.Futai,M.Maeda: "Genes of Members of the GATA-binding protein family(GATA-GT1 and GATA-GT2)together with H^+K^+-ATPase are specifically transcribed in gastric parietal cells." FEBS Lett.340. 117-120 (1994)

[Publications] M.Futai,A.Iwamoto,H.Omote,M.-Y.Park,M.Maeda: "Catalysis and energy coupling of H^+-ATPase(ATP Synthase):Molecular biological approaches." Biochimica et Biophysica Acta. 1187. 165-170 (1994)

[Publications] M.-Y.Park,H.Omote,M.Maeda,M.Futai: "Conserved Glu-181 and Arg-182 residues of Escherichia coli H^+-ATPase(ATP synthase)β subunit are essential for catalysis:Properties of 33 mutants between βGlu-161 and βLys-201 residues." J.Biochem.116. 1139-1145 (1994)

[Publications] H.Mori,T.Fukui,Y.Moriyama,M.Futai,Y.Tashiro: "Association of N-ethylmaleimide-sensitive factor with synaptic vesicles." FEBS Lett.350. 253-257 (1994)

[Publications] A.M.V.Dunean,T.Ozawa,H.Suzuki and R.Rosen: "Assignment of the gene for the cytochrome cl subunnit of the mitochondrial cytochrome bcl complex(CYCl)to human chromosome 8q24.3." Genomics. 19. 400-401 (1994)

[Publications] M.M.Islam,M.Tanaka,K.Torii,N.Hattori,T.Ozawa: "A complete cDNA sequence for core I protein subunit of human ubiquinol-cytochrome c reductase." Biochem.Mol.Biol.Int. 32. 797-805 (1994)

[Publications] K.Katsumata,M.Hayakawa,S.Sugiyama,T.Ozawa,et all.: "Fragmentation of human heart mitochondrial DNA associated with premature aging." Biochim.Biophys.Res.Commun.202. 102-110 (1994)

[Publications] A.Duncan.L.Anderson.C.duff,T.Ozawa,H.Suzuki and et all.: "Assignment of the gene(UQCRF51)for the Rieske iron-sulfur protein subunit of the mitochondrial cytochrome bcl complex to the 22q13 and 19q12-q13.1 regions of the human genome." Genomics. 21. 281-283 (1994)

[Publications] M.Tanaka and T.Ozawa: "Strand asymmetry in human mitochondrial DNA mutations." Genomics. 22. 327-335 (1994)

[Publications] W.Sato,M.Tanaka,S.Sugiyama,G.Takada,T.Ozawa and et all: "Cardiomyopathy and angiopathy in patients with mitochondrial myopathy,lactic acidosis and stroke-like episodes." Am.Heart J.128. 733-741 (1994)

[Publications] A.Iwamoto,Y.Orita,M.Maeda,and M.Futai: "N-Ethylmaleimide-sensitive mutant(βVal-153→Cys)Escherichia coli F1 ATPase:Cross-Linking of the mutant β subunit with the α subunit." FEBS Lett.352. 243-246 (1994)

[Publications] A.Kovalenko,M.Tanaka,W.Borgeld and T.Ozawa: "A common hot spot for somatic and germline mutations in rat mitochondrial DNA:Analysis by fluorescence-based sscp." Biochem.Mol.Biol.Int.34. 1205-1214 (1994)

[Publications] T.Furui,O.Kurauchi,T.Ozawa,Y.Tomoda,et all.: "Decrease in cytochrome c oxidase and cytochrome oxidase subunit I messenger ribonucleic acid levels in preeclamptic pregnancies." Obstet.Gynecol.84. 283-288 (1994)

[Publications] S.Ikebe,M.Tanaka,and T.Ozawa: "Point mutations of mitochondrial genome in parkinson's disease." Mol.Brain.Res.(in press).

[Publications] S.A.Kovalenco,M.Tanaka and T.Ozawa: "Simple methods for preparation of plasmid DNA yielding long and accurate sequence data." Nucleic.Acids.Res.(in press).

[Publications] T.Ozawa,K.Katsumata,M.Sekiguchi and et all.: "Genotype and phenotype of a severe mitochondrial cardiomyopathy:a recipient of heart transplantation and the genetic control." Biochim.Biophys.Res.Commun.(accepted).

[Publications] 伊藤雄而: "中性子小角散乱装置" 蛋白質・核酸・酵素. 39(11). 2026-2032 (1994)

[Publications] T.Uyama,Y.Moriyama,M.Futai and H.Michibata.: "Immunological detection of a vacuolar-type H^+-ATPase in vanadocytes of the ascidian ascidia sydneiensis samea." J.of Experi.Zool.207. 148-154 (1994)

[Publications] Y.Ito,M.Imai,Y.Moriyama and M.Futai: "Solution structure of F_0F_1-ATPase.Utilisation des neutrons et du rayonnement synchrotron en biologie:diffusion centrale,duffusion duffuse et diffusion inelastique." St Nizier du Moucherotte,France. Nov.21-25 (1994)

[Publications] Y.Moriyama,V.Patel,M.Futai: "Quinnacrine mustard and lipophilic entions inhibitory to both vacuolar H^+-ATPase and F_0F1-ATP synthase" FEBS Lett.359. 69-72 (1995)

[Publications] S.Ueno,K.Takeda,M.Maeda,M.Futai,M.Kawamura.: "Functional consequances of the substitution of disulfide-bonded segment,Cys127-Cys150,located in the extracellular domain of the Na,K-ATPase β subunit:Arg148 is essential for the functional expression of Na,K-ATPase." (in press).

[Publications] I.Hayashi,S.Ohta,Y.Kagawa,H.Kondo,H.Kaneda,et all: "Nuclear but not mitochondrial genome involvement in human age-related mitochondrial dysfunction." J.Biol.Chem.269(9). 6878-6883 (1994)

[Publications] T.Koike,N.Inohara,I.Sato,Y.Kagawa,S.Ohta,et all.: "Transcription of the MRP RNA gene in frog stage I oocytes requires a novel ciselement." Novel Biochim.Biophys.Res.Comun.202(1). 225-233 (1994)

[Publications] S.Akiyama,H.Endo,N.Inohara,S.Ohta and Y.Kagawa: "Gene structure and cell type-specific expression of the human ATP synthase a subunit." Biochim.Biophys.Acta.1219. 129-140 (1994)

[Publications] S.Ohta,Y.Kagawa,M.Yoshida,M.Nishizawa,et all.: "Widespread tissue distribution of multiple mitochondrial DNA deletions in familial mitochondrial myopathy." Muscle and Nerve. 17. 741-746 (1994)

[Publications] J-I.Hayashi,S.Ohta,Y.Kagawa,D.Takai,S.Miyabayashi,K.Tada: "Functional and morphological abnormalities of mitochondria in human cells containing mitochondrial DNA with pathogenic point mutations in tRNA genes." J.Biol.Chem.269(29). 19060-19066 (1994)

[Publications] K.Nakano,C.Takase,S.Nakagawa,J.Inazawa,S.Ohta: "Isolation characterization and structural organization of the gene and pseudogene for the dihydrolipoamide succinyltransferase component of the human 2-oxoglutarate dehydrogenase complex." Eur.J.Biochem.224. 179-189 (1994)

[Publications] 白岩伸子、太田成男: "ミトコンドリア脳筋症" 現代化学増刊23 遺伝子診断と遺伝子治療. 49-54 (1994)

[Publications] 太田成男: "ミトコンドリアDNAの変異によって生じるミトコンドリア病とミトコンドリアの形成制御機構" 日本生化学学会誌 生化学. (印刷中).

[Publications] 太田成男: "ミトコンドリア病の遺伝子治療" 臨床科学. 30(4). 464-468 (1994)

[Publications] Y.Orii,T.Mogi,M.Sato,T.Hirano and Y.Anraku: "Facilitated intramolecular electron transfer in the Escherichia coli bo-type ubiquinol oxidase requires chloride." Biochemistry.(in press).

[Publications] T.Mogi,K.Saiki and Y.Anraku,et all.: "Biosynthesis and functional role of heme O and heme A." Mol.Microbiol.14. 391-398 (1994)

[Publications] T.Mogi,H.Nakamura,Y.Anraku,et all.: "Molecular structure of a heme-copper redox center of the Escherichia coli ubiquinol oxidase:Evidence and model." J.Biochem.116. 741-747 (1994)

[Publications] T.Mogi,K.Matsushita,O.Adachi and Y.Anraku,et all.: "Structure-function studies on the ubiquinol oxidation site of the cytochrome bo complex from Escherichia coli using p-benzoquinones and substituted phenols." J.Biol.Chem.269. 28899-28907 (1994)

[Publications] M.Sato,T.Mogi,T.Kitagawa,H.Iwamura,Y.Anraku,et all.: "Identification of a novel quinone binding site in the cytochrome bo complex from Escherichia coli." J.Biol.Chem.269. 28908-28912 (1994)

[Publications] Y.Moriyama,Y.Tashiro,M.Takahashi,M.Maeda,M.Futai: "Microvesicles isolated from bovine posterior pituitary accumulate norepinephrine." (in press).

[Publications] K.Saiki,T.Mogi,M.Kawasaki and Y.Anraku: "An Escherichia coli cyoE gene homologue in thermophilic Bacillus PS3 encodes a thermotolerant heme O synthase." FEBS Lett.351. 385-388 (1994)

[Publications] S.Hirota,T.Mogi,T.Ogura,Y.Anraku,T.Kitagawa,et all.: "Observation of the Fe-O2 and Fe^<1V> =O Raman bands in dioxygen reduction by cytochrome bo-type ubiquinol oxidase from Escherichia coli." FEBS Lett.352. 67-70 (1994)

[Publications] Y.Orii,T.Mogi,M.Kawasaki,Y.Anraku: "Facilitated intra-molecular electron transfer in the cytochrome bo-type ubiquinol oxidase initiated upon reaction of the fully-reduced enzyme with dioxygen." FEBS Lett.352. 151-154 (1994)

[Publications] T.Uno,T.Mogi,Tsubaki,Y.Nishimura and Y.Anraku: "Resonance Raman and Fourier-transform infrared studies on the subunit I histidine mutants of the cytochrome bo complex in Escherichia coli:Molecular structure of redox centers." J.Biol.Chem.269. 11912-11920 (1994)

[Publications] 茂木立志: "大腸菌チトクロムbo型ユビキノール酸化酵素の構造と分子生理" 生化学. 67(2)(印刷中).

[Publications] T.Uyama,Y.Moriyama,M.Futai and H.Michibata: "Immunological detection of a vacuolar-type H^+-ATPase in vanadocytes of the ascidian Ascidia sydneiensis samea." J.Exp.Zool. 270. 148-154 (1994)

[Publications] K.Kanamori and H.Michibata: "Raman specroscopic study of the vanadium and sulfate in blood cell homogenates of the ascidian,Ascidia gemmata." J.Mar.Biol.Ass.U.K.74. 279-286 (1994)

[Publications] I.Inoue and T.Higuti: "ATP-sensitive K^+-channel in the mitochondrial inner membrane in:handbook of molecular and cellular physiology of membrane channels." Ed.Peracchia,C.(Invited review)Academic Press. 549-554 (1994)

[Publications] 道端 斉: "原索動物ホヤによるバナジウムの還元濃縮機構" 生化学. 66. 495-509 (1994)

[Publications] K.Mimura,M.Tsujimoto,H.Arai,K.Inoue,et all.: "Chinese hamster ovary cells expressing a novel type of acetylated LDL receptor." J.Biol.Chem. In press.

[Publications] Y.Sato,K.Nishikawa,H.Arai,K.Murofushi,K.Inoue,et all.: "Side chain structure is critical for transport of sterols from lysosomes to cytoplasm." Biochem.Biophys.Acta. in press.

[Publications] K.Aikawa,Y.Fujimoto,H.Arai and K.Inoue,et all.: "Structure-specific inhibition of lysosomal cholesterol transport in macrophages by various steroids." Biochim.Biophys.Acta.1213. 127-134 (1994)

[Publications] 新井洋由: "コレステロールの細胞内輸送機構" 蛋白質・核酸・酵素. 39(9). 1518-1531 (1994)

[Publications] A.Tohgo,N.Noguchi,Y.Furuya,H.Yonekura,H.Okamoto,et all.: "Essential cysteine residues for cyclic ADP-ribose synthesis and hydrolysis by CD38." J.Biol.Chem.269. 28555-28557 (1994)

[Publications] O.Tanaka,H.Kondo,S.Takasawa,T.Yoshimoto,H.Okamoto,et all.: J.Biol.Chem.269. 21223-21228 (1994)

[Publications] M.Unno,O.Tanaka,A.J.Bone,S.Takasawa,H.Okamoto,et all.: "Pancreatic beta-cell replication and amelioration of surgical diabetes by Reg protein." Proc.Natl.Acad.Sci.U.S.A.91. 3589-3592 (1994)

[Publications] S.-H.Hua,Y.Furuya,M.Nohmi,H.Okamoto,K.Kuba,et all.: "Cyclic ADP-ribose modulates Ca2-release channels for activation by physiological Ca2-entry in bullfrog sympathetic neurons." Neuron. 12. 1073-1079 (1994)

[Publications] H.Higashida,S.Takasawa,H.Okamoto,D.A.Brown,et all.: "Nicotinamide adenine dinucleotide regulates muscarinic receptor-coupled K+(M)channels in rodent NG 108-15 cells." J.Physiol.482. 317-323 (1995)

[Publications] S.Takasawa,A.Tohgo,Y.Furuya,H.Yonekura,H.Okamoto,et all.: "Cloning and characterization of cDNA encoding rat ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase(homologue to human CD38)from islets of langerhans." Biochim.Biophys.Acta.1223. 160-162 (1994)

[Publications] Y.Hirata,Y.Ohsugi,H.Okamoto,T.Kaisho,T.Hirano,et all.: "ADP ribosyl cyclase activity of a novel bone marrow stromal cell surface molecule,BST-1." FEBS Lett.356. 244-248 (1994)

[Publications] A.Berlova,A.Tohgo,T.Karasawa,T.Takeuchi,H.Okamoto,et all.: "Assignment of the CD38 gene encoding human leukocyte antigen cd38(ADP-ribosyl cyclase/Cyclic ADP-ribose hydrolase)to chromosome 4p15." Cytogenet.Cell Genet.69. 38-39 (1995)

[Publications] K.Nakagawara,H.Miyashita,H.Yonekura,H.Okamoto,et all.: "Isolation,structural determination and expression of a novel rag gene,human rag Iβ." Biochim.Biophys.Acta.1217. 199-202 (1994)

[Publications] A.Tohgo,H.Yonekura,N.Hayashi,H.Okamoto,et all.: "Structural determination and characterization of a 40 kDa protein isolated from 40S ribosomal subunit." FEBS Lett.340. 133-138 (1994)

[Publications] T.Watanabe,M.Unno,H.Miyashita,H.Okamoto,et all.: "Structure and expression of a novel rat reg III gene." Gene. 144. 315-316 (1994)

[Publications] H.Yonekura,Y.Suzuki,S.Takasawa,H.Okamoto,et all.: "Characterization of the 5'-regulatory region of the rat Reg I gene." Biochim.Biophys.Acta.1219. 241-243 (1994)

[Publications] A.Nagai,H.Matsumiya,S.Yasui,H.Okamoto,K.Konno,et all.: "Effects of nicotinamide and niacin on bleomycin-induced acute injury and subsequent fibrosis in hamster lungs." Experimental Lung Research.20. 263-281 (1994)

[Publications] T.Numakunai,K.Shikama,S.Takasawa,H.Okamoto,et all.: "The structure of the Aplysia kurodai gene encoding ADP-ribosyl cyclase,a secondmessenger enzyme." Gene.(in press).

[Publications] H.Okamoto: "New aspects to the functioning and regeneration of pancreatic β-cells:Cyclic ADP-ribose and reg gene." Islet Physiology and Pathophysiology,Plenum Press,N.Y.in press.

[Publications] H.Okamoto,S.Takasawa and A.Tohgo: "New aspects to the physiological significance of NAD,poly ADP-ribose and cyclic ADP-ribose." Biochim. (in press).

[Publications] H.Okamoto,S.Takasawa,I.Kato,N.Noguchi and H.Yonekura,et all.: "A role of cyclic ADP-ribose in stimulus-secretion coupling in pancreatic β-cells." Elsevier,Amsterdam. (in press).

[Publications] H.Yonekura,M.Unno,Y.Suzuki,H.Miyashita,H.Okamoto,et all.: "Structure and expression of reg gene family." Smith-Gordon,London. (in press).

[Publications] 岡本宏: "膵β細胞破壊と防止についての岡本モデルとその展開" 糖尿病. 37. 99-102 (1994)

[Publications] 高沢伸、那谷耕司、杉本孝子、武山淳二、米倉秀人、岡本宏他: "膵β細胞機能発現の新しい細胞内情報伝達物質cyclic ADP-ribose" 糖尿病記録号1993. 247-251 (1994)

[Publications] 加藤一郎、鈴木雄、赤羽敦也、米倉秀人、岡本宏、田中理: "膵ランゲルハンス島β細胞でVIP遺伝子を過剰発現するトランスジェニックマウスの作成と導入遺伝子による血糖降下作用" 分子糖尿病学. 5. 73-80 (1994)

[Publications] K.Saika,K.Inaka,T.Matsui,M.Yoshida and K.Miki: "Crystallization of mutant β subunit of F1-ATPase from therinophilic bacillus PS3" J.Mol.Biol.242. 709-711 (1994)

[Publications] 三木邦夫: "タンパク質結晶学と構造生物学の新たな飛躍" 化学. 49(8). 592-593 (1994)

[Publications] J.Ezaki,L.S.Wolfe,T.Higuti,K.Ishidoh,and E.Kominami: "Specific delay of degradation of mitochondrial ATP synthase subunit c in late infantile neuronal ceroid-lipofuscinosis(Batten Disease)." J.Neurochemistrry. 64(2)(in press).

二井將光大阪大学, 産業科学研究所, 教授 (50012646)

[Publications] 太田成男: "ミトコンドリアDNAの変異によって生じるミトコンドリア病とミトコンドリアの形成制御機構" 日本生化学学会誌生化学. (印刷中).

[Publications] 道端斉: "原索動物ホヤによるバナジウムの還元濃縮機構" 生化学. 66. 495-509 (1994)