1993 Fiscal Year Annual Research Report

中枢グルタミン酸受容体の分子構造と生理機能

Research Project

Project/Area Number	04267103
Research Institution	Niigata University
Principal Investigator	三品昌美新潟大学, 脳研究所, 教授 (80144351)
Co-Investigator(Kenkyū-buntansha)	渡辺雅彦北海道大学, 医学部, 助教授 (70210945) 相沢慎一新潟大学, 理化学研究所, 副主任研究員 (60073011) 杉山博之九州大学, 理学部, 教授 (20124224) 津本忠治大阪大学, 医学部, 教授 (50028619) 小澤瀞司群馬大学, 医学部, 教授 (40049044)
Keywords	グルタミン酸受容体 / NMDA受容体チャネル / 脳 / 長期増強 / スライス / 標的遺伝子組換え / パッチクランプ / 海馬
Research Abstract	1.4種類のε/ζNMDA受容体チャネルは非競争的阻害剤MK-801に対して感受性を異にすることを見い出した。また、NMDA受容体チャネルがTPA処理により活性化され、ε2サブユニットのM4領域よりカルボキシル末端側が活性調節に関与していることを明らかにした。さらに、マウス脳より新たなグルタミン酸受容体チャネルサブユニットδ2を見い出し、小脳プルキニエ細胞のみに局在していることを明らかにした。2.海馬スライス標本を用いて、PIP_2抗体により長期増強が消失することを見い出した。また、虚血後の海馬に持続の長いAMPA受容体チャネル電流を見い出した。3.パッチクランプ法とRT-PCR法により、培養海馬ニューロンの二種類のAMPA受容体チャネルはサブユニット構成の違いに基づくことを明らかにした。4.ラット視覚野切片におけるシナプス長期増強の誘発にはシナプス後部の脱分極が必要であり、そのいき値が-40mV程度であることを見い出した。5.代謝調節型グルタミン酸受容体のサブタイプ1の発現が神経回路形成期におこることを見い出し、さらのサブタイプ5の全一次構造を明らかにした。6.後根神経節、交感・副交感神経節における種々のグルタミン酸受容体の発現を解析した。また、グルタミン酸トランスポーターが主としてグリア細胞と脳室周囲器官の細胞に発現していることを明らかにした。7.非受容体型チロシンキナーゼfyn欠損マウスはMGCおよび海馬領域に組織学的変化を有し、授乳行動の異常を示すことを見い出した。8.多様なNMDA受容体チャネルのサブユニットmRNAの成熟マウス脳の前脳領域における発現分布を詳細に解析した。9.バキュロウイルスベクターを用いてNMDA受容体チャネルのζ1サブユニットの大量発現系を確立した。10.標的遺伝子組換えによりNMDA受容体チャネルのサブユニット遺伝子を欠損したマウスを得た。

Research Products

(62 results)

All Other

All Publications (62 results)

[Publications] Nakano,R.et al.: "A novel mutation in Cu/Zn superoxide dismutase gene in Japanese familial amyotrophic lateral sclerosis" Biochem.Biophys.Res.Commun.(in press). (1994)
[Publications] Ishida,N.et al.: "Circadian expression of NMDA recptor mRNAs,ε3 and ζ1,in the suprachiasmatic nucleus of rat brain" Neurosci.Lett.(in press). (1994)
[Publications] Watanabe,M.et al.: "Differential distributions of the NMDA receptor channel subunit mRNAs in the mouse retina." Brain Res.634. 328-332 (1994)
[Publications] Araki,K.et al.: "Selective expression of the glutamate receptor channel δ2 subunit in cerebellar Purkinje cells." Biochem.Biophtys.Res.Commun.197. 1267-1276 (1993)
[Publications] Takano,H.et al.: "Chromosomal localization of the ε1,ε3 and ζ1 subunit genes of the human NMDA receptor channel." Biochem.Biophtys.Res.Commun.197. 922-926 (1993)
[Publications] Yamakura,T.et al.: "Phosphorylation of the carboxyl-terminal domain of the ζ1 subunit is not responsible for potentiation by TPA of the NMDA receptor channel." Biochem.Biophtys.Res.Commun.196. 1537-1544 (1993)
[Publications] Watanabe,M.et al.: "Distinct distributions of five N-methyl-D-aspartate receptor channel subunit mRNAs in the forebrain." J.Comp.Neurol.338. 377-390 (1993)
[Publications] Watanabe,M.et al.: "Distinct spatio-temporal distributions of the NMDA receptor channel subunit mRNAs in the brain." Ann.NY Acad.Sci.707. 463-466 (1993)
[Publications] Kawamoto,S.et al.: "Expression of the α1 and α2 subunits of the AMPA-selective glutamate receptor channel in insect cells using a Baculovirus vector" Ann.NY Acad.Sci.707. 460-462 (1993)
[Publications] Mishina,M.et al.: "Molecular and functional diversity of the NMDA receptor channel" Ann.NY Acad.Sci.707. 136-152 (1993)
[Publications] Yamakura,T.et al.: "Different sensitivities of NMDA receptor channel subtypes to noncompetitive antagonists" NeuroReport. 4. 687-690 (1993)
[Publications] Mori,H.et al.: "Involvement of the carboxyl-terminal region in modulation by TPA of the NMDA receptor channel" NeuroReport. 4. 519-522 (1993)
[Publications] Tsubokawa,H.et al.: "Block of long-term potentiation by intracellular application of anti-phosphatidylinositol 4,5-bisphosphate antibody in hippocampal pyramidal neurons." Neuroscience. 55. 643-651 (1993)
[Publications] Miwa,A.et al.: "Presynaptic glutamate receptors depress inhibitory postsynaptic transmission in lobster neuromuscular synapse." J.Neurophysiol.70. 1159-1167 (1993)
[Publications] Robinson,H.P.C.et al.: "Injection of digitally synthesized synaptic conductance transients to measure the integrative properties of neurons." J.Neurosci.Methods. 46. 157-165 (1993)
[Publications] Tsubokawa,H.et al.: "Inositol 1,3,4,5-tetrakisphosphate (IP_4) as a mediator of neuronal death in ischemic hippocampus." Neuroscience. (in press).
[Publications] Shimazaki,K.et al.: "Changes in inositol 1,4,5-triphosphate 3-kinase immunoreactivity following transient ischemia in gerbil hippocampus." NeuroReport. (in press).
[Publications] Tsubokawa,H.et al.: "Ca^<2+>-dependent non-NMDA receptor-mediated synaptic currents in ischemic CA1 hippocampal neurons." J.Neurophysiol.(in press).
[Publications] Ozawa,S.and Iino,M.: "Two distinct types of AMPA responses in cultured rat hippocampal neurons" Neuroscience Letters. 115. 187-190 (1993)
[Publications] Ozawa,S.: "Glutamate receptor channels in hippocampal neurons" Japanese Journal of Physiology. 43. 141-159 (1993)
[Publications] Bochet,P.et al.: "Subunit composition at the single cell level explains functional properties of a glutamate-gated channel" Neuron. (in press). (1994)
[Publications] 小澤瀞司: "海馬ニューロンの興奮性シナプス" 神経研究の進歩. 38(1). 23-33 (1994)
[Publications] Yoshimura,Y & Tsumoto,T.: "Dependence of LTP induction on postsynaptic depolarization:A perforated patch clamp study in visual cortical slices" Journal of Neurophysiology. in press. (1994)
[Publications] Funauchi,Y.et al.: "Effects of an inhibitor for calcium/calmodulin-dependent protein phosphatase,calcineurin,on induction of long-term potentiation in rat visual cortex" Neuroscience Research. in press. (1994)
[Publications] Sato,H.et al.: "Broad-tuned chromatic inputs to color-selective neurons in the monkey visual cortex" Journal of Neurophysiology. in press. (1994)
[Publications] Takahashi,M.P.et al.: "Laminar difference in tetanus-induced increase of intracellular Ca^<2+> in visual cortex of young rats" Neuroscience Research. 17. 217-228 (1993)
[Publications] Takahashi,M.P.et al.: "Contribution of NMDA receptors to tetanus-induced increase in postsynaptic Ca^<2+> in visual cortex of young rats" Neuroscience Research. 17. 229-239 (1993)
[Publications] Hata,Y.et al.: "Development of local horizontal interactions in cat visual cortex studied by cross-correlation analysis" Journal of Neurophysiology. 69. 40-56 (1993)
[Publications] Minakami,R.,Katsuki,F.and Sugiyama,H.: "A variant of metabotropic glutamate receptor subtype 5:an evolutionary conserved insertion with no termination codon" Biochem.Biophys.Res.Commun.194. 622-627 (1993)
[Publications] Araki,T.,Kenimer,J.,Nishimune,A.,Sugiyama,H.,ら: "Identification of the metabotropic glutamate receptor-1 protein in the rat trigeminal ganglion" Brain Research. 627. 341-344 (1993)
[Publications] Sugiyama,H.,Nakamura,K.and Nukada,T.: "Mode of interactions between metaborropic glutamate receptors and G proteins in Xenopus ooctye" Annals of NY Academy of Science. 707. 515-518 (1993)
[Publications] Nakamura,K.,Nukada,T.,Haga,T.& Sugiyama,H.: "G protein-mediated inhibition of phosphoinositide metabolism evoked by metabotropic glutamate receptors in frog oocytes" J.Physiol.(Lond.). 474. 35-41 (1994)
[Publications] Toru Shida,et.al.: "Expression of muscarinic and nicotinic receptor mINA in the salivary gland of rats;a study by in situ hybridization histochemistry." Molecular Brain Research. 17. 335-339 (1993)
[Publications] Tatsuya Asai,et al.: "Differential expression of two members of FGF receptor gene family FGFR-1 and FGFR-2 mRNA,in the adult rat central nervous system." Molecular Brain Research. 17. 174-178 (1993)
[Publications] Gui Lan Yao,et.al.: "Distribution of GAP-43(B50/F1) mRNA in the adult rat brain by in situ hybridization using an alkaline phosphatase labeled probe" Molecular Brain Research. 18. 1-16 (1993)
[Publications] Hiroshi Maeno,et al.: "Distribution of the substance P receptor (NK-1 receptor) in the central nervous system." Molecular Brain Research. 18. 43-58 (1993)
[Publications] Emiko Senba,et al.: "Stress-induced c-fos expression in the rat Brain;activation mechanism of sympathetic pathway." Brain Research Bull.31. 329-344 (1993)
[Publications] Masahiro Fujata,et al.: "Ontogeny of dopamine transporter mRNA expression in the rat brain." Molecular Brain Research. 19. 222-236 (1993)
[Publications] Takanori Saika,et al.: "Changes in preproenkephalin mRNA after unilateral and bilateral labyrinthectomy in the rat medial vestibular nucleus." Molecular Brain Research. 19. 237-240 (1993)
[Publications] Hiroshi Kiyama,et al.: "Sympathetic and parasympathetic ganglia express non-NMDA type glutamate receptors;distinct receptor subunit composition in the principle and SIF cells." Molecular Brain Research. 19. 345-348 (1993)
[Publications] Hiroshi Kiyama,et al.: "Charateristic localization of non-NMDA type glutamate receptor subunits in the rat pituitary gland." Molecular Brain Research. 19. 262-268 (1993)
[Publications] Tatsuo Furuyama,et al.: "Region-specific expression of subunits of ionotropic glutamate receptors(AMPA-type,KA-type and NMDA receptors)in the rat spinal cord with special reference to nociception." Molecular Brain Research. 18. 141-151 (1993)
[Publications] Akio Wanaka,et al.: "Developmentally regulated expressio of pleiotrophin,a novel heparin binding growth factor,in the nervous system of the rat." Developmental Brain Research. 72. 133-144 (1993)
[Publications] Mariko Yamano,et al.: "The innervation of calcitonin gene-related peptide to the Purkinje cells and granule cells in the developing mouse cerebellum" Developmental Brain Research. 72. 107-117 (1993)
[Publications] Koji Sato,et al.: "The differential expression patterns of messenger RNAs encoding non-N-methyl-D-asparatate glutamate receptor subunits(Glurl-4) in the rat brain." Neuroscience. 52. 515-539 (1993)
[Publications] Masahiro Fujita,et al.: "Cellular localization of serotonin transporter mRNA in the rat brain." Neuroscience Letters. 162. 59-62 (1993)
[Publications] Furuta,Y.et al.: "MDS-like experimental myelodysplasia:multilineage abnormal hematopoiesis in transgenic mice harbaring the SV40 large T antigen under an immunoglobulin" Exp.Hematol.21. 806-815 (1993)
[Publications] Nada,S.et al.: "Constitutive activation of Src family kinases in mouse embryos that lack Csk" Cell. 73. 1125-1135 (1993)
[Publications] Imoto,S.et al.: "Regulation of class II NHC gene expression by the inducible antisense RNA in transgenic mice" Anal.Biochem.210. 231-234 (1993)
[Publications] Furuta,Y.et al.: "Degeneration of skeletal and cardiac muscles in c-myb-transgenic mice" Transgenic Res.2. 199-207 (1993)
[Publications] Yagi,T.et al.: "A novle negative selection for homologous recombinants using diphtheria toxin A franment gene" Anal.Biochem.214. 77-86 (1993)
[Publications] Yagi,T.et al.: "A novel ES cell strain,TT2,with high germline-differentiating potency" Anal.Biochem.214. 68-76 (1993)
[Publications] Yagi,T.et al.: "Regional localization of Fyn in adult brain:Studies with mice in which fyn gene was replaced by lacZ" Oncogene. 8. 3343-3351 (1993)
[Publications] Tsukada,T.et al.: "Enhanced proliferative potential in culture of cells from P53-deficient mice" Oncogene. 8. 3313-3322 (1993)
[Publications] Yagi,T.et al.: "A role for Fyn tyrosine kinase in the suckling behavior of neonatal mice" Nature. 366. 742-745 (1993)
[Publications] 中村健司他: "自在な標的遺伝子置換法" 実験医学. 11(20). 138-141 (1993)
[Publications] 権藤洋一他: "トランスジェニックマウスを用いた変異原の高感度解析法の開発" 環境変異原研究. 15. 39-49 (1993)
[Publications] Gondo,Y.et al.: "High-frequency genetic reversion mediated by a DNA duplication:Mouse pink-eyed unstable mutation" Proc.Natul.Acad.Sci.U.S.A.90. 297-301 (1993)
[Publications] Kawai,N.& Nakajima,T.: "Natural and Synthetic Neurotoxins (ed.by A.Harvey)" Academic Press, 27(319-345) (1993)
[Publications] Kawai,N.: "Aspects of synaptic transmission (ed.by T.W.Stone)" Taylor & Francis, 10(221-230) (1993)
[Publications] 杉山博之: "「生体における情報伝達」(分担執筆)" 南江堂(西塚泰美・田中千賀子編集), 322 (1993)
[Publications] 伊藤功,杉山博之: "「レセプター ----基礎と臨床----」(分担執筆)" 朝倉書店(井村裕夫・岡哲雄・芳賀達也・岸本英爾編集), 944 (1993)

1993 Fiscal Year Annual Research Report

中枢グルタミン酸受容体の分子構造と生理機能

Principal Investigator

三品 昌美 新潟大学, 脳研究所, 教授 (80144351)

Research Products

[Publications] Nakano,R.et al.: "A novel mutation in Cu/Zn superoxide dismutase gene in Japanese familial amyotrophic lateral sclerosis" Biochem.Biophys.Res.Commun.(in press). (1994)

[Publications] Ishida,N.et al.: "Circadian expression of NMDA recptor mRNAs,ε3 and ζ1,in the suprachiasmatic nucleus of rat brain" Neurosci.Lett.(in press). (1994)

[Publications] Watanabe,M.et al.: "Differential distributions of the NMDA receptor channel subunit mRNAs in the mouse retina." Brain Res.634. 328-332 (1994)

[Publications] Araki,K.et al.: "Selective expression of the glutamate receptor channel δ2 subunit in cerebellar Purkinje cells." Biochem.Biophtys.Res.Commun.197. 1267-1276 (1993)

[Publications] Takano,H.et al.: "Chromosomal localization of the ε1,ε3 and ζ1 subunit genes of the human NMDA receptor channel." Biochem.Biophtys.Res.Commun.197. 922-926 (1993)

[Publications] Yamakura,T.et al.: "Phosphorylation of the carboxyl-terminal domain of the ζ1 subunit is not responsible for potentiation by TPA of the NMDA receptor channel." Biochem.Biophtys.Res.Commun.196. 1537-1544 (1993)

[Publications] Watanabe,M.et al.: "Distinct distributions of five N-methyl-D-aspartate receptor channel subunit mRNAs in the forebrain." J.Comp.Neurol.338. 377-390 (1993)

[Publications] Watanabe,M.et al.: "Distinct spatio-temporal distributions of the NMDA receptor channel subunit mRNAs in the brain." Ann.NY Acad.Sci.707. 463-466 (1993)

[Publications] Kawamoto,S.et al.: "Expression of the α1 and α2 subunits of the AMPA-selective glutamate receptor channel in insect cells using a Baculovirus vector" Ann.NY Acad.Sci.707. 460-462 (1993)

[Publications] Mishina,M.et al.: "Molecular and functional diversity of the NMDA receptor channel" Ann.NY Acad.Sci.707. 136-152 (1993)

[Publications] Yamakura,T.et al.: "Different sensitivities of NMDA receptor channel subtypes to noncompetitive antagonists" NeuroReport. 4. 687-690 (1993)

[Publications] Mori,H.et al.: "Involvement of the carboxyl-terminal region in modulation by TPA of the NMDA receptor channel" NeuroReport. 4. 519-522 (1993)

[Publications] Tsubokawa,H.et al.: "Block of long-term potentiation by intracellular application of anti-phosphatidylinositol 4,5-bisphosphate antibody in hippocampal pyramidal neurons." Neuroscience. 55. 643-651 (1993)

[Publications] Miwa,A.et al.: "Presynaptic glutamate receptors depress inhibitory postsynaptic transmission in lobster neuromuscular synapse." J.Neurophysiol.70. 1159-1167 (1993)

[Publications] Robinson,H.P.C.et al.: "Injection of digitally synthesized synaptic conductance transients to measure the integrative properties of neurons." J.Neurosci.Methods. 46. 157-165 (1993)

[Publications] Tsubokawa,H.et al.: "Inositol 1,3,4,5-tetrakisphosphate (IP_4) as a mediator of neuronal death in ischemic hippocampus." Neuroscience. (in press).

[Publications] Shimazaki,K.et al.: "Changes in inositol 1,4,5-triphosphate 3-kinase immunoreactivity following transient ischemia in gerbil hippocampus." NeuroReport. (in press).

[Publications] Tsubokawa,H.et al.: "Ca^<2+>-dependent non-NMDA receptor-mediated synaptic currents in ischemic CA1 hippocampal neurons." J.Neurophysiol.(in press).

[Publications] Ozawa,S.and Iino,M.: "Two distinct types of AMPA responses in cultured rat hippocampal neurons" Neuroscience Letters. 115. 187-190 (1993)

[Publications] Ozawa,S.: "Glutamate receptor channels in hippocampal neurons" Japanese Journal of Physiology. 43. 141-159 (1993)

[Publications] Bochet,P.et al.: "Subunit composition at the single cell level explains functional properties of a glutamate-gated channel" Neuron. (in press). (1994)

[Publications] 小澤瀞司: "海馬ニューロンの興奮性シナプス" 神経研究の進歩. 38(1). 23-33 (1994)

[Publications] Yoshimura,Y & Tsumoto,T.: "Dependence of LTP induction on postsynaptic depolarization:A perforated patch clamp study in visual cortical slices" Journal of Neurophysiology. in press. (1994)

[Publications] Funauchi,Y.et al.: "Effects of an inhibitor for calcium/calmodulin-dependent protein phosphatase,calcineurin,on induction of long-term potentiation in rat visual cortex" Neuroscience Research. in press. (1994)

[Publications] Sato,H.et al.: "Broad-tuned chromatic inputs to color-selective neurons in the monkey visual cortex" Journal of Neurophysiology. in press. (1994)

[Publications] Takahashi,M.P.et al.: "Laminar difference in tetanus-induced increase of intracellular Ca^<2+> in visual cortex of young rats" Neuroscience Research. 17. 217-228 (1993)

[Publications] Takahashi,M.P.et al.: "Contribution of NMDA receptors to tetanus-induced increase in postsynaptic Ca^<2+> in visual cortex of young rats" Neuroscience Research. 17. 229-239 (1993)

[Publications] Hata,Y.et al.: "Development of local horizontal interactions in cat visual cortex studied by cross-correlation analysis" Journal of Neurophysiology. 69. 40-56 (1993)

[Publications] Minakami,R.,Katsuki,F.and Sugiyama,H.: "A variant of metabotropic glutamate receptor subtype 5:an evolutionary conserved insertion with no termination codon" Biochem.Biophys.Res.Commun.194. 622-627 (1993)

[Publications] Araki,T.,Kenimer,J.,Nishimune,A.,Sugiyama,H.,ら: "Identification of the metabotropic glutamate receptor-1 protein in the rat trigeminal ganglion" Brain Research. 627. 341-344 (1993)

[Publications] Sugiyama,H.,Nakamura,K.and Nukada,T.: "Mode of interactions between metaborropic glutamate receptors and G proteins in Xenopus ooctye" Annals of NY Academy of Science. 707. 515-518 (1993)

[Publications] Nakamura,K.,Nukada,T.,Haga,T.& Sugiyama,H.: "G protein-mediated inhibition of phosphoinositide metabolism evoked by metabotropic glutamate receptors in frog oocytes" J.Physiol.(Lond.). 474. 35-41 (1994)

[Publications] Toru Shida,et.al.: "Expression of muscarinic and nicotinic receptor mINA in the salivary gland of rats;a study by in situ hybridization histochemistry." Molecular Brain Research. 17. 335-339 (1993)

[Publications] Tatsuya Asai,et al.: "Differential expression of two members of FGF receptor gene family FGFR-1 and FGFR-2 mRNA,in the adult rat central nervous system." Molecular Brain Research. 17. 174-178 (1993)

[Publications] Gui Lan Yao,et.al.: "Distribution of GAP-43(B50/F1) mRNA in the adult rat brain by in situ hybridization using an alkaline phosphatase labeled probe" Molecular Brain Research. 18. 1-16 (1993)

[Publications] Hiroshi Maeno,et al.: "Distribution of the substance P receptor (NK-1 receptor) in the central nervous system." Molecular Brain Research. 18. 43-58 (1993)

[Publications] Emiko Senba,et al.: "Stress-induced c-fos expression in the rat Brain;activation mechanism of sympathetic pathway." Brain Research Bull.31. 329-344 (1993)

[Publications] Masahiro Fujata,et al.: "Ontogeny of dopamine transporter mRNA expression in the rat brain." Molecular Brain Research. 19. 222-236 (1993)

[Publications] Takanori Saika,et al.: "Changes in preproenkephalin mRNA after unilateral and bilateral labyrinthectomy in the rat medial vestibular nucleus." Molecular Brain Research. 19. 237-240 (1993)

[Publications] Hiroshi Kiyama,et al.: "Sympathetic and parasympathetic ganglia express non-NMDA type glutamate receptors;distinct receptor subunit composition in the principle and SIF cells." Molecular Brain Research. 19. 345-348 (1993)

[Publications] Hiroshi Kiyama,et al.: "Charateristic localization of non-NMDA type glutamate receptor subunits in the rat pituitary gland." Molecular Brain Research. 19. 262-268 (1993)

[Publications] Tatsuo Furuyama,et al.: "Region-specific expression of subunits of ionotropic glutamate receptors(AMPA-type,KA-type and NMDA receptors)in the rat spinal cord with special reference to nociception." Molecular Brain Research. 18. 141-151 (1993)

[Publications] Akio Wanaka,et al.: "Developmentally regulated expressio of pleiotrophin,a novel heparin binding growth factor,in the nervous system of the rat." Developmental Brain Research. 72. 133-144 (1993)

[Publications] Mariko Yamano,et al.: "The innervation of calcitonin gene-related peptide to the Purkinje cells and granule cells in the developing mouse cerebellum" Developmental Brain Research. 72. 107-117 (1993)

[Publications] Koji Sato,et al.: "The differential expression patterns of messenger RNAs encoding non-N-methyl-D-asparatate glutamate receptor subunits(Glurl-4) in the rat brain." Neuroscience. 52. 515-539 (1993)

[Publications] Masahiro Fujita,et al.: "Cellular localization of serotonin transporter mRNA in the rat brain." Neuroscience Letters. 162. 59-62 (1993)

[Publications] Furuta,Y.et al.: "MDS-like experimental myelodysplasia:multilineage abnormal hematopoiesis in transgenic mice harbaring the SV40 large T antigen under an immunoglobulin" Exp.Hematol.21. 806-815 (1993)

[Publications] Nada,S.et al.: "Constitutive activation of Src family kinases in mouse embryos that lack Csk" Cell. 73. 1125-1135 (1993)

[Publications] Imoto,S.et al.: "Regulation of class II NHC gene expression by the inducible antisense RNA in transgenic mice" Anal.Biochem.210. 231-234 (1993)

[Publications] Furuta,Y.et al.: "Degeneration of skeletal and cardiac muscles in c-myb-transgenic mice" Transgenic Res.2. 199-207 (1993)

[Publications] Yagi,T.et al.: "A novle negative selection for homologous recombinants using diphtheria toxin A franment gene" Anal.Biochem.214. 77-86 (1993)

[Publications] Yagi,T.et al.: "A novel ES cell strain,TT2,with high germline-differentiating potency" Anal.Biochem.214. 68-76 (1993)

[Publications] Yagi,T.et al.: "Regional localization of Fyn in adult brain:Studies with mice in which fyn gene was replaced by lacZ" Oncogene. 8. 3343-3351 (1993)

[Publications] Tsukada,T.et al.: "Enhanced proliferative potential in culture of cells from P53-deficient mice" Oncogene. 8. 3313-3322 (1993)

[Publications] Yagi,T.et al.: "A role for Fyn tyrosine kinase in the suckling behavior of neonatal mice" Nature. 366. 742-745 (1993)

[Publications] 中村健司 他: "自在な標的遺伝子置換法" 実験医学. 11(20). 138-141 (1993)

[Publications] 権藤洋一他: "トランスジェニックマウスを用いた変異原の高感度解析法の開発" 環境変異原研究. 15. 39-49 (1993)

[Publications] Gondo,Y.et al.: "High-frequency genetic reversion mediated by a DNA duplication:Mouse pink-eyed unstable mutation" Proc.Natul.Acad.Sci.U.S.A.90. 297-301 (1993)

[Publications] Kawai,N.& Nakajima,T.: "Natural and Synthetic Neurotoxins (ed.by A.Harvey)" Academic Press, 27(319-345) (1993)

[Publications] Kawai,N.: "Aspects of synaptic transmission (ed.by T.W.Stone)" Taylor & Francis, 10(221-230) (1993)

[Publications] 杉山博之: "「生体における情報伝達」(分担執筆)" 南江堂(西塚泰美・田中千賀子 編集), 322 (1993)

[Publications] 伊藤功,杉山博之: "「レセプター ----基礎と臨床----」(分担執筆)" 朝倉書店(井村裕夫・岡哲雄・芳賀達也・岸本英爾 編集), 944 (1993)

三品昌美新潟大学, 脳研究所, 教授 (80144351)

[Publications] 中村健司他: "自在な標的遺伝子置換法" 実験医学. 11(20). 138-141 (1993)

[Publications] 杉山博之: "「生体における情報伝達」(分担執筆)" 南江堂(西塚泰美・田中千賀子編集), 322 (1993)

[Publications] 伊藤功,杉山博之: "「レセプター ----基礎と臨床----」(分担執筆)" 朝倉書店(井村裕夫・岡哲雄・芳賀達也・岸本英爾編集), 944 (1993)