1994 Fiscal Year Annual Research Report

中枢グルタミン酸受容体の分子構造と生理機能

Research Project

Project/Area Number	04267103
Research Institution	The University of Tokyo
Principal Investigator	三品昌美東京大学, 医学部(医), 教授 (80144351)
Co-Investigator(Kenkyū-buntansha)	遠山正彌大阪大学, 医学部, 教授 (40028593) 杉山博之九州大学, 理学部, 教授 (20124224) 津本忠治大阪大学, 医学部, 教授 (50028619) 小澤瀞司群馬大学, 医学部, 教授 (40049044) 川合述史自治医科大学, 教授 (00073065)
Keywords	グルタミン酸受容体 / NMDA受容体チャネル / 脳 / 長期増強 / スライス / 標的遺伝子組換え / パッチクランプ / 海馬
Research Abstract	本研究班は、中枢における神経情報伝達シナプス可塑性の鍵を握るグルタミン酸受容体を実体として明らかにするとともにその生理機能を分子構造と対応させて明確にすることを目標とした。研究計画が順調に進行していることから、本年度は生理機能を分子レベルで理解することに重点をおいて、以下の成果を挙げた。NMDA受容体チャネルのε1サブユニット遺伝子を不活化されたマウスを作成し、海馬シナプスにおける長期増強と空間学習の低下が起こることを見い出した。この成果は、記憶・学習のシナプス可塑性仮説を支持するものである(三品)。短時間虚血負荷後のスナネズミ海馬錘体細胞においてAMPA型グルタミン酸受容体チャネル電流の持続性やカルシウム透過性が変化することを単一チャネル電流の解析により明らかにした(川合)。海馬切片を対象として、スライスパッチクランプ法とRT-PCR法を組み合わせることにより、グルタミン酸受容体チャネルの電気生理学的特性とサブユニット構成との関連を明らかにした(小澤)。ラット視覚野から調整した培養神経細胞標本に穿孔パッチクランプ法を適用することにより、視覚野におけるシナプス長期増強の誘発にはシナプス後細胞の脱分極と同期したシナプス前部の賦括が必要であることを見い出した(津本)。代謝型グルタミン酸受容体のサブタイプ5にはスプライシングの違いによる2種類の型が存在し、発生の時期により調節されていることを見い出した(杉山)。AMPA型グルタミン酸名受容体チャネルmRNAがラットの腺細胞に発現していることならびに虚血条件下ではグルタミン酸トランスポーターの遺伝子発現と蛋白の細胞内局在が変化することを見い出した(遠山)。ホメオ蛋白Otx2遺伝子のヘテロ欠損マウスを作製し、Otx2が頭蓋においても神経系においても下顎セグメントの形態形成を特異的に制御することを明らかにした(相沢)。5種類のNMDA受容体チャネルサブユニットの遺伝子発現パターンは生後2週間の間に、領域差の小さい胎児型から顕著な空間的異質性を特徴とする成熟型へと変換することを明らかにした(渡辺)。バキュロウイルス発現系により大量発現させたAMPA型およびNMDA型グルタミン酸受容体チャネルを用いて、リガンド結合活性にN-グリコシル化が必須であることを明らかにした(川本)。

Research Products

(67 results)

All Other

All Publications (67 results)

[Publications] Watanabe,M.: "Differential distributions of the NMDA receptor channel subunit mRNAs in the mouse retina." Brain Res.634. 328-332 (1994)
[Publications] Watanabe,M.: "Distinct gene expression of the N-methyl-D-aspartate receptor channel subunit in peripheral neurons of the mouse sensory ganglia and adrenal gland." Neurosci. Lett.165. 183-186 (1994)
[Publications] Ishida,N.: "Circadian expression of NMDA receptor mRNAs, ε3 and ζ1, in the suprachiasmatic nucleus of rat brain." Neurosci. Lett.166. 211-215 (1994)
[Publications] Watanabe,M.: "Distinct spatio-temporal expressions of five NMDA receptor channel subunit mRNAs in the cerebellum." J. Comp. Neurol.343. 513-519 (1994)
[Publications] Watanabe,M.: "Distinct distributions of five NMDA receptor channel subunit mRNAs in the brainstem." J. Comp. Neurol.343. 520-531 (1994)
[Publications] Watanabe,M.: "Distinct spatio-temporal distributions of the NMDA receptor channel subunit mRNAs in the mouse cervical cord." J. Comp. Neurol.345. 314-320 (1994)
[Publications] Nakano,R.: "A novel mutation in Cu/Zn superoxide dismutase gene in Japanese familial amyotrophic lateral sclerosis." Biochem. Biophys. Res. Commun.200. 695-703 (1994)
[Publications] Kawamoto,S.: "Ligand binding properties and N-glycosylation of the α-amino-3-hydroxy-5-methyl-4-isoxazole propionate(AMPA)-selective glutamate receptor channel expressed in a Baculovirus system." Eur. J. Biochem.223. 665-673 (1994)
[Publications] Takahasi,H.: "Familial amyotrophic lateral sclerosis with a mutation in the Cu/Zn superoxide dismutase gene." Acta Neuropathol.88. 185-188 (1994)
[Publications] Tsuzuki,K.: "Ion permeation properties of the cloned mouse ε2/ζ1 NMDA receptor channel." Mol. Brain Res.26. 37-46 (1994)
[Publications] Hattori,S.: "Expression and characterization of the α2 subunit of the α-amino-3-hydroxy-5-methyl-4-isoxazole propionate(AMPA)-selective glutamate receptor channel in a Baculovirus system." Brain Res.666. 43-52 (1994)
[Publications] Mori,H.: "Different spatio-temporal expressions of three otx homeoprotein transcripts during zebrafich embryogenesis." Mol. Brain Res.27. 221-231 (1994)
[Publications] Hirano,T.: "Involvement of the glutamate receptor δ2 subunit in the long-term depression of glutamete responsiveness in cultured Purkinje cells." Neurosci. Lett.182. 172-176 (1994)
[Publications] Sakimura,K.: "Reduced hippocampal LTP and spatial learning of mice lacking NMDA receptor ε1 subunit." Nature. 373. 151-155 (1995)
[Publications] Hirano,T.: "Suppression of LTD in cultured Purkinji cells deficient of glutamate receptor δ2 subunit." NeuroReport. (印刷中). (1995)
[Publications] Kawamoto,S.: "N-linked glycosylation of the α-amino-3-hydroxy-5-methyl-4-isoxazole propionate(AMPA)-selective glutamate receptor channel α2 subunit is essential for the acquisition of ligand-binding activity." J.Neurochem.(印刷中). (1995)
[Publications] Kawamoto,S.: "Expression and characterization of the ζ1 subunit of the N-methyl-D-aspartate(NMDA) receptor channel in a Baculovirus system." Mol. Brain Res.(印刷中). (1995)
[Publications] Iino,M.: "Relationship between calcium permeability and rectification properties of AMPA receptors in cultured rat hippocampal neurons." Neurosci. Lett.173. 14-16 (1994)
[Publications] Tsuzuki,K.: "Ion permeation properties of the cloned mouse ε2/ζ1 NMDA receptor channel." Mol. Brain Res.26. 37-46 (1994)
[Publications] Inoue,T.: "Experimental model for MDS-like myelodysplasia in transgenic mice harboring the SV40 large-T antigen under an immunoglobulin enhancer." Leukemia(Suppliment). 8. 202-205 (1994)
[Publications] Hashimoto,N.: "Parthenogenetic activation of oocytes in c-mos-deficient mice." Nature. 370. 68-71 (1994)
[Publications] Yagi,T.: "Fyn expression during early neurogenesis in mouse embryos." Oncogene. 9. 2433-2440 (1994)
[Publications] Tamemoto,H.: "Inslin resistance and growth retardation in mice lacking inslin receptor substrate-1." Nature. 372. 182-185 (1994)
[Publications] Aizawa,S.: "Non-receptor tyrosine kinases in mammalian neurogenesis." Proceedings of the Princess Takamatsu Symposium. 24. 323-337 (1994)
[Publications] Yoshida,M.: "A new strategy of gene-trapping in ES cells using 3'-RACE." Transgenic Research. 4(印刷中). (1995)
[Publications] Nakamura,K.: "G protein-mediated inhibition of phosphoinositide metabolism evoked by metabotropic glutamate receptors in frog oocytes." J. Physiol. (Lond.). 474. 35-41 (1994)
[Publications] Sugiyama,H.: "Molecular and functional properties of metabotropic glutamate receptors in the brain." Biomed Res.15. 29-31 (1994)
[Publications] Minakami,R.: "Molecular cloning and the functional expression of two isoforms of human metabotropic glutamate receptor subtype 5." Biochem. Biophys. Res. Commun.199. 1136-1143 (1994)
[Publications] Kasahara,J.: "Inositol phospholipid metabolism in Xenopus oocytes mediated by endogenous Go and Gi proteins." FEBS Lett.355. 41-44 (1994)
[Publications] Tsubokawa,H.: "Inositol 1,3,4,5-tetrakisphosphate(IP_4) as a mediator of neuronal death in ischemic hippocampus." Neurosi.59. 291-297 (1994)
[Publications] Shimazaki,K.: "Changes in inositol 1,4,5-triphosphate 3-kinase immunoreactivity following transient ischemia in gerbil hippocampus." NeuroReport. 5. 785-788 (1994)
[Publications] Tsubokawa,H.: "Ca^<2+>-dependent non-NMDA receptor-mediated synaptic currents in ischemic CA1 hippocampal neurons." J. Neurophysiol.71. 1190-1196 (1994)
[Publications] Shimazaki,K.: "Increase in bc1-2 oncoprotein and the tolerance to ischemia-induced neuronal death in the gerbli hippocampus." Neurosci. Res.20. 95-99 (1994)
[Publications] Ishida,A.: "An electrophysiological and immunohistochemical study of the hippocampus of the reelermutant mouse." Brian Res.662. 60-68 (1994)
[Publications] Yoshimura,Y.: "Dependance of LTP induction on postsynaptic depolarization: A proforated patch clamp study in visual cortical slices of young rats." J. Neurophysiol.71. 1638-1645 (1994)
[Publications] Funauchi,M.: "Effects of an inhibitor for calcium/calmodulin-dependent protein phosphatase calcineurin, on induction of long-term potentiation in rat visual cortex." Neurosci. Res.19. 269-278 (1994)
[Publications] Sato,H.: "Broad-tuned chromatic inputs to color-selective neurons in the monkey visual cortex." J. Neurophysiol.72. 163-168 (1994)
[Publications] Lin,M.-H.: "Intracellular calcium increase induced by GABA in visual cortex of fetal and neonatal rats and its disappearance with development." Neurosci. Res.20. 85-94 (1994)
[Publications] Haruta,H.: "Induction of LTD but not LTP through metabotropic glutamate receptors in visual cortex." NeuroReport. 5. 1829-1832 (1994)
[Publications] Takahashi,Y.: "NMDA receptors contribute to afferent synaptic transmission in the medial vestibular nucleus of young rats." Brain Res.659. 287-291 (1994)
[Publications] Takahashi,Y.: "NMDA receptor-mediated increase of intraneuronal Ca^<2+> in the medial vestibular nucleus of young rats." Neurosci. Res.21. 59-70 (1994)
[Publications] Torii,N.: "An inhibitor for calcium, FK506, blocks induction of long-term depression in rat visual cortex." Neurosci. Lett.184(印刷中). (1994)
[Publications] Kamishita,T.: "Metabotropic glutamate receptors and visual cortical synaptic plasticity." Canadian J. Physiol. Pharmacol.73(印刷中). (1995)
[Publications] Watanabe,M.: "Molecular cloning of rat cDNAs for ζ and θ subtypes of 14-3-3 protein and differential distributions of their mRNAs in the brain." Mol. Brain Res.25. 113-121 (1994)
[Publications] Watanabe,M.: "Differential expressions of the topoisomerase IIα and IIβ mRNAs in developing rat brain." Neurosci. Res.19. 51-57 (1994)
[Publications] Fujita,S.: "Expression of NMDA receptor subunit mRNA in the vestibular ganglion of the rat and guinea-pig." NeuroReport. 5. 862-864 (1994)
[Publications] Tohyama,M.: "Gene expression of neuroreceptors in the locus coeruleus of the rat." Microscopy Reseach and Technique. 29. 200-203 (1994)
[Publications] Sakaguchi,T.: "Expression of basic fibroblast growth factor receptor messenger RNA in the periinfarcted brain tissue." Restorative Neurology and Neuroscience.7. 29-36 (1994)
[Publications] Mushiake,S.: "Gene for members of the GATA-binding protein family(GATA-GT1 and GATA-GT2) together with H+/K+-ATPase are spesifically transcribed in gastric parietal cells." FEBS Lett.340. 117-120 (1994)
[Publications] Yuguchi,T.: "Messenger RNA and protein expression of vasic fibroblast growth factor receptor after cortical ablation." Mol. Brain Res.25. 50-56 (1994)
[Publications] Fujita,M.: "Distribution of cocaine recognition sites in rat brain : in vitro and ex vivo autoradiography with [125I]RTI-55." J. Chem. Neuroanat.7. 13-23 (1994)
[Publications] Kobayashi,N.: "GAP-43(B50/F1) gene regulation by axonal injury of the hypoglossal nerve in the adult rat." Mol. Brain Res.21. 9-18 (1994)
[Publications] Ohno,K.: "Occurrence of galanin-like immunoreactivity in vestibular and cochlear efferent neurons after labyrinthectomy in the rat." Brain Res.644. 135-143 (1994)
[Publications] Otori,Y.: "Marked increase in glutamate-aspartate transporter(GLAST/CluT-1) mRNA following transient retinal ischemia." Mol. Brain Res.27. 310-314 (1994)
[Publications] Matsumoto,K.: "A novel family of heparin-binding growth factors, pleiotrophin and midkine, is expressed in the developing rat cerebral cortex." Dev. Brain Res.79. 229-241 (1994)
[Publications] Mushiake,S.: "Two-dimensional demonstration of myenteric nerve plexus : Application for pseudo-hirschsprung's disease." J. of Pediatric Surgery. 29. 1472-1482 (1994)
[Publications] Matsumoto,K.: "Localization of pleiotrophin and midkine in the postnatal developing cerebellum." Neurosci. Lett.178. 216-220 (1994)
[Publications] Yamano,M.: "Distribution of corticotropin-releasing factor and calcitonin gene related peptide in the developing mouse cerebellum." Neurosci. Res.19. 387-396 (1994)
[Publications] Shimizu,H.: "Effect of Dibutyryl Cyclic AMP on Morphologic Features and Marker Production of Human Gervical Argyrophil Small-Cell Carcinoma Cell Line." Int. J. Cancer. 56. 766-771 (1994)
[Publications] Saika,T.: "Differential regulation of phospholopase Cisozymes in the rat facial nucleus following axotomy." Neurosci.59. 121-129 (1994)
[Publications] Kondo,E.: "Coexpression of GABAA receptor g1 and g2 subunits in the rat trigeminal ganglion." Mol. Brain Res.21. 363-367 (1994)
[Publications] Imaizumi,K.: "Differential expression of skg mRNA, a member of the Ser/Thr protein kinase gene family, in rat brain after CNS injury." Mol. Brain Res.26. 189-196 (1994)
[Publications] Kiyama,H.: "Growth Factors and Extracellular Signal-Rugulated Kinases (Mitogen-Activated Protein Kinase) in the Rat Pineal Gland." Neuroendocrinology. 59. 152-155 (1994)
[Publications] Ohno,K.: "Up-regulation of GAP-43(B50/F1) gene expression in vestibular efferent neurons following labyrinthectomy in the rat : In situ hybridization using an alkaline phosphatese-labeled probe." Hearing Res.80. 123-127 (1994)
[Publications] Inoue,K.: "Cloning and expression of a bovine glutamate transporter." Mol. Brain Res.(印刷中). (1995)
[Publications] Adams,R.H.: "Gene structure and glial expression of the glycine transporter GlyT1 in emryonic and adult rodents." J. Neurosci.(印刷中). (1995)
[Publications] 川合述史: "分子から見た脳" 講談社, 212 (1994)

1994 Fiscal Year Annual Research Report

中枢グルタミン酸受容体の分子構造と生理機能

Principal Investigator

三品 昌美 東京大学, 医学部(医), 教授 (80144351)

Research Products

[Publications] Watanabe,M.: "Differential distributions of the NMDA receptor channel subunit mRNAs in the mouse retina." Brain Res.634. 328-332 (1994)

[Publications] Watanabe,M.: "Distinct gene expression of the N-methyl-D-aspartate receptor channel subunit in peripheral neurons of the mouse sensory ganglia and adrenal gland." Neurosci. Lett.165. 183-186 (1994)

[Publications] Ishida,N.: "Circadian expression of NMDA receptor mRNAs, ε3 and ζ1, in the suprachiasmatic nucleus of rat brain." Neurosci. Lett.166. 211-215 (1994)

[Publications] Watanabe,M.: "Distinct spatio-temporal expressions of five NMDA receptor channel subunit mRNAs in the cerebellum." J. Comp. Neurol.343. 513-519 (1994)

[Publications] Watanabe,M.: "Distinct distributions of five NMDA receptor channel subunit mRNAs in the brainstem." J. Comp. Neurol.343. 520-531 (1994)

[Publications] Watanabe,M.: "Distinct spatio-temporal distributions of the NMDA receptor channel subunit mRNAs in the mouse cervical cord." J. Comp. Neurol.345. 314-320 (1994)

[Publications] Nakano,R.: "A novel mutation in Cu/Zn superoxide dismutase gene in Japanese familial amyotrophic lateral sclerosis." Biochem. Biophys. Res. Commun.200. 695-703 (1994)

[Publications] Kawamoto,S.: "Ligand binding properties and N-glycosylation of the α-amino-3-hydroxy-5-methyl-4-isoxazole propionate(AMPA)-selective glutamate receptor channel expressed in a Baculovirus system." Eur. J. Biochem.223. 665-673 (1994)

[Publications] Takahasi,H.: "Familial amyotrophic lateral sclerosis with a mutation in the Cu/Zn superoxide dismutase gene." Acta Neuropathol.88. 185-188 (1994)

[Publications] Tsuzuki,K.: "Ion permeation properties of the cloned mouse ε2/ζ1 NMDA receptor channel." Mol. Brain Res.26. 37-46 (1994)

[Publications] Hattori,S.: "Expression and characterization of the α2 subunit of the α-amino-3-hydroxy-5-methyl-4-isoxazole propionate(AMPA)-selective glutamate receptor channel in a Baculovirus system." Brain Res.666. 43-52 (1994)

[Publications] Mori,H.: "Different spatio-temporal expressions of three otx homeoprotein transcripts during zebrafich embryogenesis." Mol. Brain Res.27. 221-231 (1994)

[Publications] Hirano,T.: "Involvement of the glutamate receptor δ2 subunit in the long-term depression of glutamete responsiveness in cultured Purkinje cells." Neurosci. Lett.182. 172-176 (1994)

[Publications] Sakimura,K.: "Reduced hippocampal LTP and spatial learning of mice lacking NMDA receptor ε1 subunit." Nature. 373. 151-155 (1995)

[Publications] Hirano,T.: "Suppression of LTD in cultured Purkinji cells deficient of glutamate receptor δ2 subunit." NeuroReport. (印刷中). (1995)

[Publications] Kawamoto,S.: "N-linked glycosylation of the α-amino-3-hydroxy-5-methyl-4-isoxazole propionate(AMPA)-selective glutamate receptor channel α2 subunit is essential for the acquisition of ligand-binding activity." J.Neurochem.(印刷中). (1995)

[Publications] Kawamoto,S.: "Expression and characterization of the ζ1 subunit of the N-methyl-D-aspartate(NMDA) receptor channel in a Baculovirus system." Mol. Brain Res.(印刷中). (1995)

[Publications] Iino,M.: "Relationship between calcium permeability and rectification properties of AMPA receptors in cultured rat hippocampal neurons." Neurosci. Lett.173. 14-16 (1994)

[Publications] Tsuzuki,K.: "Ion permeation properties of the cloned mouse ε2/ζ1 NMDA receptor channel." Mol. Brain Res.26. 37-46 (1994)

[Publications] Inoue,T.: "Experimental model for MDS-like myelodysplasia in transgenic mice harboring the SV40 large-T antigen under an immunoglobulin enhancer." Leukemia(Suppliment). 8. 202-205 (1994)

[Publications] Hashimoto,N.: "Parthenogenetic activation of oocytes in c-mos-deficient mice." Nature. 370. 68-71 (1994)

[Publications] Yagi,T.: "Fyn expression during early neurogenesis in mouse embryos." Oncogene. 9. 2433-2440 (1994)

[Publications] Tamemoto,H.: "Inslin resistance and growth retardation in mice lacking inslin receptor substrate-1." Nature. 372. 182-185 (1994)

[Publications] Aizawa,S.: "Non-receptor tyrosine kinases in mammalian neurogenesis." Proceedings of the Princess Takamatsu Symposium. 24. 323-337 (1994)

[Publications] Yoshida,M.: "A new strategy of gene-trapping in ES cells using 3'-RACE." Transgenic Research. 4(印刷中). (1995)

[Publications] Nakamura,K.: "G protein-mediated inhibition of phosphoinositide metabolism evoked by metabotropic glutamate receptors in frog oocytes." J. Physiol. (Lond.). 474. 35-41 (1994)

[Publications] Sugiyama,H.: "Molecular and functional properties of metabotropic glutamate receptors in the brain." Biomed Res.15. 29-31 (1994)

[Publications] Minakami,R.: "Molecular cloning and the functional expression of two isoforms of human metabotropic glutamate receptor subtype 5." Biochem. Biophys. Res. Commun.199. 1136-1143 (1994)

[Publications] Kasahara,J.: "Inositol phospholipid metabolism in Xenopus oocytes mediated by endogenous Go and Gi proteins." FEBS Lett.355. 41-44 (1994)

[Publications] Tsubokawa,H.: "Inositol 1,3,4,5-tetrakisphosphate(IP_4) as a mediator of neuronal death in ischemic hippocampus." Neurosi.59. 291-297 (1994)

[Publications] Shimazaki,K.: "Changes in inositol 1,4,5-triphosphate 3-kinase immunoreactivity following transient ischemia in gerbil hippocampus." NeuroReport. 5. 785-788 (1994)

[Publications] Tsubokawa,H.: "Ca^<2+>-dependent non-NMDA receptor-mediated synaptic currents in ischemic CA1 hippocampal neurons." J. Neurophysiol.71. 1190-1196 (1994)

[Publications] Shimazaki,K.: "Increase in bc1-2 oncoprotein and the tolerance to ischemia-induced neuronal death in the gerbli hippocampus." Neurosci. Res.20. 95-99 (1994)

[Publications] Ishida,A.: "An electrophysiological and immunohistochemical study of the hippocampus of the reelermutant mouse." Brian Res.662. 60-68 (1994)

[Publications] Yoshimura,Y.: "Dependance of LTP induction on postsynaptic depolarization: A proforated patch clamp study in visual cortical slices of young rats." J. Neurophysiol.71. 1638-1645 (1994)

[Publications] Funauchi,M.: "Effects of an inhibitor for calcium/calmodulin-dependent protein phosphatase calcineurin, on induction of long-term potentiation in rat visual cortex." Neurosci. Res.19. 269-278 (1994)

[Publications] Sato,H.: "Broad-tuned chromatic inputs to color-selective neurons in the monkey visual cortex." J. Neurophysiol.72. 163-168 (1994)

[Publications] Lin,M.-H.: "Intracellular calcium increase induced by GABA in visual cortex of fetal and neonatal rats and its disappearance with development." Neurosci. Res.20. 85-94 (1994)

[Publications] Haruta,H.: "Induction of LTD but not LTP through metabotropic glutamate receptors in visual cortex." NeuroReport. 5. 1829-1832 (1994)

[Publications] Takahashi,Y.: "NMDA receptors contribute to afferent synaptic transmission in the medial vestibular nucleus of young rats." Brain Res.659. 287-291 (1994)

[Publications] Takahashi,Y.: "NMDA receptor-mediated increase of intraneuronal Ca^<2+> in the medial vestibular nucleus of young rats." Neurosci. Res.21. 59-70 (1994)

[Publications] Torii,N.: "An inhibitor for calcium, FK506, blocks induction of long-term depression in rat visual cortex." Neurosci. Lett.184(印刷中). (1994)

[Publications] Kamishita,T.: "Metabotropic glutamate receptors and visual cortical synaptic plasticity." Canadian J. Physiol. Pharmacol.73(印刷中). (1995)

[Publications] Watanabe,M.: "Molecular cloning of rat cDNAs for ζ and θ subtypes of 14-3-3 protein and differential distributions of their mRNAs in the brain." Mol. Brain Res.25. 113-121 (1994)

[Publications] Watanabe,M.: "Differential expressions of the topoisomerase IIα and IIβ mRNAs in developing rat brain." Neurosci. Res.19. 51-57 (1994)

[Publications] Fujita,S.: "Expression of NMDA receptor subunit mRNA in the vestibular ganglion of the rat and guinea-pig." NeuroReport. 5. 862-864 (1994)

[Publications] Tohyama,M.: "Gene expression of neuroreceptors in the locus coeruleus of the rat." Microscopy Reseach and Technique. 29. 200-203 (1994)

[Publications] Sakaguchi,T.: "Expression of basic fibroblast growth factor receptor messenger RNA in the periinfarcted brain tissue." Restorative Neurology and Neuroscience.7. 29-36 (1994)

[Publications] Mushiake,S.: "Gene for members of the GATA-binding protein family(GATA-GT1 and GATA-GT2) together with H+/K+-ATPase are spesifically transcribed in gastric parietal cells." FEBS Lett.340. 117-120 (1994)

[Publications] Yuguchi,T.: "Messenger RNA and protein expression of vasic fibroblast growth factor receptor after cortical ablation." Mol. Brain Res.25. 50-56 (1994)

[Publications] Fujita,M.: "Distribution of cocaine recognition sites in rat brain : in vitro and ex vivo autoradiography with [125I]RTI-55." J. Chem. Neuroanat.7. 13-23 (1994)

[Publications] Kobayashi,N.: "GAP-43(B50/F1) gene regulation by axonal injury of the hypoglossal nerve in the adult rat." Mol. Brain Res.21. 9-18 (1994)

[Publications] Ohno,K.: "Occurrence of galanin-like immunoreactivity in vestibular and cochlear efferent neurons after labyrinthectomy in the rat." Brain Res.644. 135-143 (1994)

[Publications] Otori,Y.: "Marked increase in glutamate-aspartate transporter(GLAST/CluT-1) mRNA following transient retinal ischemia." Mol. Brain Res.27. 310-314 (1994)

[Publications] Matsumoto,K.: "A novel family of heparin-binding growth factors, pleiotrophin and midkine, is expressed in the developing rat cerebral cortex." Dev. Brain Res.79. 229-241 (1994)

[Publications] Mushiake,S.: "Two-dimensional demonstration of myenteric nerve plexus : Application for pseudo-hirschsprung's disease." J. of Pediatric Surgery. 29. 1472-1482 (1994)

[Publications] Matsumoto,K.: "Localization of pleiotrophin and midkine in the postnatal developing cerebellum." Neurosci. Lett.178. 216-220 (1994)

[Publications] Yamano,M.: "Distribution of corticotropin-releasing factor and calcitonin gene related peptide in the developing mouse cerebellum." Neurosci. Res.19. 387-396 (1994)

[Publications] Shimizu,H.: "Effect of Dibutyryl Cyclic AMP on Morphologic Features and Marker Production of Human Gervical Argyrophil Small-Cell Carcinoma Cell Line." Int. J. Cancer. 56. 766-771 (1994)

[Publications] Saika,T.: "Differential regulation of phospholopase Cisozymes in the rat facial nucleus following axotomy." Neurosci.59. 121-129 (1994)

[Publications] Kondo,E.: "Coexpression of GABAA receptor g1 and g2 subunits in the rat trigeminal ganglion." Mol. Brain Res.21. 363-367 (1994)

[Publications] Imaizumi,K.: "Differential expression of skg mRNA, a member of the Ser/Thr protein kinase gene family, in rat brain after CNS injury." Mol. Brain Res.26. 189-196 (1994)

[Publications] Kiyama,H.: "Growth Factors and Extracellular Signal-Rugulated Kinases (Mitogen-Activated Protein Kinase) in the Rat Pineal Gland." Neuroendocrinology. 59. 152-155 (1994)

[Publications] Ohno,K.: "Up-regulation of GAP-43(B50/F1) gene expression in vestibular efferent neurons following labyrinthectomy in the rat : In situ hybridization using an alkaline phosphatese-labeled probe." Hearing Res.80. 123-127 (1994)

[Publications] Inoue,K.: "Cloning and expression of a bovine glutamate transporter." Mol. Brain Res.(印刷中). (1995)

[Publications] Adams,R.H.: "Gene structure and glial expression of the glycine transporter GlyT1 in emryonic and adult rodents." J. Neurosci.(印刷中). (1995)

[Publications] 川合述史: "分子から見た脳" 講談社, 212 (1994)

三品昌美東京大学, 医学部(医), 教授 (80144351)