1993 Fiscal Year Annual Research Report

光合成の環境適応の分子機構

Research Project

Project/Area Number	04273103
Research Institution	Okazaki National Research Institutes
Principal Investigator	村田紀夫岡崎国立共同研究機構, 基礎生物学研究所, 教授 (90011569)
Co-Investigator(Kenkyū-buntansha)	篠崎一雄理化学研究所, ライフサイエンス筑波研究センター, 主任研究員 (20124216) 藤伊正筑波大学, 生物科学系, 教授 (20011611) 杉山達夫名古屋大学, 農学部, 教授 (50023453) 水野猛名古屋大学, 農学部, 教授 (10174038) 和田敬四郎金沢大学, 理学部, 教授 (70028174)
Keywords	環境適応 / 低温 / 塩ストレス / 乾燥 / 無機イオン欠乏 / ラン藻
Research Abstract	研究目的:低温、塩、無イオン欠乏党の環境要因の変化への適応現象を分子レベルで解明すること、および適応能力のすぐれた形質転換植物を作成することを全体の目的とする。本年度は、環境適応に関与する遺伝子のクローニングを重要目標とし、さらにクローニングされた遺伝子の解析と、形質転換植物作成のための準備をおこなうことを目標とした。主要研究成果:(1)低温適応において重要な役割を担うDELTA12不飽和化酵素をE.coli中で大量発現させた。生じたDELTA12不飽和化酵素はフェレドキシンを電子受容体とすること、18:1(9)を18:1(9,12)に不飽和化するが18:1(11)は不飽和化しないことを明かにした。さらに部位特異的変異法により、鉄のリガンドを形成すると推定されるヒスチジン残基の内の5残基をアルギニンに変換した。この変換により不飽和化活性は消失した。(2)塩耐性に関与すると推定されるグリシンベタインを、E.coliのbet遺伝子オペロンをシアノバクテリアPCC7942に導入することによって細胞内に相当量を合成させた。またコムギのベタインアルデヒド脱水素酵素をタバコに導入して発現させ、外からベタインアルデヒドを添加することによってベタインを生合成させることができるようになった。(3)リン酸の欠乏を感知するセンサーおよびこれによってリン酸化を受ける転写制御因子のそれぞれの遺伝子を単離した。またこれらの因子によって誘導される2種類のタンパク質を同定したが、これらのタンパク質はリン酸の取り込み機構を形成していると推定された。

Research Products

(27 results)

All Other

All Publications (27 results)

[Publications] Vigh,L.: "The primary signal in the biological perception of temperature:Pd‐catalyzed hydrogenation of membrane lipids stimulated the expression of the desA gene in Synechocystis PCC6803." Proc.Natl.Acad.Sci.U.S.A.90. 9090-9094 (1993)
[Publications] Wada,H.: "The contribution of membrane‐lipids to the ability of the photosynthetic machinery to tolerate temperature stress." Proc.Natl.Acad.Sci.U.S.A.in press. (1993)
[Publications] Mamedov,Mahir.: "Effects of glycinebetatine and unsaturation of membrane lipids on heat stability of photosynethetic electron‐transport and phosphorylation reactions in Synechocystis PCC6803." Biochem.Biophs.Actra. 1142. 1-5 (1993)
[Publications] Nishiyama,Y.: "Photosynthetic adaptation to high temperature associated with thylakoid membranes of Synechococcus PCC7002." Plant Cell Physiol.34. 337-343 (1993)
[Publications] Los,D.: "The temperature‐dependent expression of the desaturase gene desA in Synechocystis PCC803." FEBS Letters. 318. 57-60 (1993)
[Publications] Wada,H.: "The desA gene of the cyanobacterium Synechocystis sp.strain PCC6803 is the structural gene for DELTA12 desaturase." J.Bacteriol.175. 6056-6058 (1993)
[Publications] Sakamoto,T.: "Identification of conserved domains in the DELTA 12 desaturase of cyanobacteria." Plant Mol.Biol.in press. (1993)
[Publications] Morisaki,N.: "Effect of side‐chain structure on inhibition of yeast fatty‐acid synthase by cerulenin analogues." Eur.J.Biochem.211. 111-115 (1993)
[Publications] Ishitani,M.: "Regulation of glycinebetaine accumulation in the halotolerant cyanobacterium Aphanothece halophytica." Aust.J.Plant Physiol.20. 693-703 (1993)
[Publications] Ishitani,M.: "Betainealdehyde dehydrogenase in the gramineae:Levels in leaves of both betaine‐accumulating and nonaccumulating cereal plants ^1." Plant Cell Physiol. 34. 493-495 (1993)
[Publications] Kishitani,S.: "Accumulation of glycinebetaine during cold acclimation and freezing tolerance in leaves of winter and spring bareley plants." Plant,Cell and Environment. 17. 89-95 (1993)
[Publications] Aiba,H.: "Sensor and regulator proteins from cyanobacterium Synechococcus species PCC7942 that belong to the bacterial signaltransduction protein families:implication in the adaptive response to phosphate limitation." Mol.Microbiol.8. 81-91 (1993)
[Publications] Nagaya,M.: "Cloning of a sensory‐kinase‐encoding gene that belongs to the two‐component regulatory family from the cyanobacterium Synechococcus sp.PCC7942." Gene. 131. 119-124 (1993)
[Publications] Kanamura,K.: "The cyanobacterium,Synechococcus sp.PCC7942,possesses two distinct genes encoding cation‐transporting P‐type ATPases." FEBS Letters. 330. 99-104 (1993)
[Publications] Nagaya,M.: "A cyanobacterial gene encoding a protein with extensive homology to mammalian phosphoribosylpyrophosphate synthetase." Biosci.Biotech.Biochem.57. 1958-1959 (1993)
[Publications] Yamaguchi‐Shinozaki,K.: "Characterization of the expression of a desiccation‐responsive rD29 gene of Arabidopsis thaliana and analysis of its promoter in transgenic plants." Mol.Gen.Genet.236. 331-340 (1993)
[Publications] Yamaguchi‐Shinozaki,K.: "Arabidopsis DNA encoding two desiccation‐responsive rd29 genes^<1.>" Plant Physiol.101. 1119‐1120 (1993)
[Publications] Kiyosue,T.: "Characterization of two cDNAs(ERD11 and ERD13)for dehydration‐inducible genes that encode putative glutathione S‐transferases in Arabidopsis thaliana L." FEBS Letters. 335. 189-192 (1993)
[Publications] Yamaguchi‐Shinozaki,K.: "The plant hormone abscisic acid mediates the drought‐induced expression but not the seed‐specific expression of rd22,a gene responsive to dehydration stress in Arabidopsis thaliana." Mol.Gen.Genet.238. 17-25 (1993)
[Publications] Urao,T.: "An Arabidopsis myb homolog is induced by dehydration stress and its gene product binds to the conserved MYB recognition sequence." Plant Cell. 5. 1529-1539 (1993)
[Publications] Kiyosue,T.: "Characterization of cDNA for a dehydration‐inducible gene that encodes a clp A,B‐like protein in Arabidopsis thaliana L." Biochem.Biophys.Res.Commun.196. 1214-1220 (1993)
[Publications] Koizumi,M.: "Structure and expression of two genes that encode distinct drought‐inducible cysteine protcinascs in Arabidopsis thaliana." Gene. 129. 175-182 (1993)
[Publications] Mi,H.: "Electron donation form cyclic and respiratory flows to photosynthetic intersystem chain is mediated by pyridine nucleotid dehydrogenase in the cyanobacterium Synechocy,stis PCC6803." Plant Cell Physiol.33. 1233-1237 (1993)
[Publications] Ogawa,T.: "Isolation.and characterization of the ccmM gene required by the cyanobacterium Synechocystis PCC6803 for inorganic carbon utilization" Photosyn.Res.in press. (1993)
[Publications] Ogawa,T.: "Cloning of a gene(ccmA)encoding a carboxysomal constituent of Synechocystis PCC6803." J.Bacteriol.in press. (1993)
[Publications] Mi,H.: "NAD(P)H dehydrogenase‐dependent cyclic electron flow around photosystem I in the cyanobacterium Synechocystis PCC6803:A study of dark‐starved cells and spheroplasts." Plant Cell Physiol.in press. (1993)
[Publications] Wada,M.: "Molecular cloning of P‐type ATPase from marine alga Heterosigma akashiwo." Plant Mol.Biol.in press. (1993)

1993 Fiscal Year Annual Research Report

光合成の環境適応の分子機構

Principal Investigator

村田 紀夫 岡崎国立共同研究機構, 基礎生物学研究所, 教授 (90011569)

Research Products

[Publications] Vigh,L.: "The primary signal in the biological perception of temperature:Pd‐catalyzed hydrogenation of membrane lipids stimulated the expression of the desA gene in Synechocystis PCC6803." Proc.Natl.Acad.Sci.U.S.A.90. 9090-9094 (1993)

[Publications] Wada,H.: "The contribution of membrane‐lipids to the ability of the photosynthetic machinery to tolerate temperature stress." Proc.Natl.Acad.Sci.U.S.A.in press. (1993)

[Publications] Mamedov,Mahir.: "Effects of glycinebetatine and unsaturation of membrane lipids on heat stability of photosynethetic electron‐transport and phosphorylation reactions in Synechocystis PCC6803." Biochem.Biophs.Actra. 1142. 1-5 (1993)

[Publications] Nishiyama,Y.: "Photosynthetic adaptation to high temperature associated with thylakoid membranes of Synechococcus PCC7002." Plant Cell Physiol.34. 337-343 (1993)

[Publications] Los,D.: "The temperature‐dependent expression of the desaturase gene desA in Synechocystis PCC803." FEBS Letters. 318. 57-60 (1993)

[Publications] Wada,H.: "The desA gene of the cyanobacterium Synechocystis sp.strain PCC6803 is the structural gene for DELTA12 desaturase." J.Bacteriol.175. 6056-6058 (1993)

[Publications] Sakamoto,T.: "Identification of conserved domains in the DELTA 12 desaturase of cyanobacteria." Plant Mol.Biol.in press. (1993)

[Publications] Morisaki,N.: "Effect of side‐chain structure on inhibition of yeast fatty‐acid synthase by cerulenin analogues." Eur.J.Biochem.211. 111-115 (1993)

[Publications] Ishitani,M.: "Regulation of glycinebetaine accumulation in the halotolerant cyanobacterium Aphanothece halophytica." Aust.J.Plant Physiol.20. 693-703 (1993)

[Publications] Ishitani,M.: "Betainealdehyde dehydrogenase in the gramineae:Levels in leaves of both betaine‐accumulating and nonaccumulating cereal plants ^1." Plant Cell Physiol. 34. 493-495 (1993)

[Publications] Kishitani,S.: "Accumulation of glycinebetaine during cold acclimation and freezing tolerance in leaves of winter and spring bareley plants." Plant,Cell and Environment. 17. 89-95 (1993)

[Publications] Aiba,H.: "Sensor and regulator proteins from cyanobacterium Synechococcus species PCC7942 that belong to the bacterial signaltransduction protein families:implication in the adaptive response to phosphate limitation." Mol.Microbiol.8. 81-91 (1993)

[Publications] Nagaya,M.: "Cloning of a sensory‐kinase‐encoding gene that belongs to the two‐component regulatory family from the cyanobacterium Synechococcus sp.PCC7942." Gene. 131. 119-124 (1993)

[Publications] Kanamura,K.: "The cyanobacterium,Synechococcus sp.PCC7942,possesses two distinct genes encoding cation‐transporting P‐type ATPases." FEBS Letters. 330. 99-104 (1993)

[Publications] Nagaya,M.: "A cyanobacterial gene encoding a protein with extensive homology to mammalian phosphoribosylpyrophosphate synthetase." Biosci.Biotech.Biochem.57. 1958-1959 (1993)

[Publications] Yamaguchi‐Shinozaki,K.: "Characterization of the expression of a desiccation‐responsive rD29 gene of Arabidopsis thaliana and analysis of its promoter in transgenic plants." Mol.Gen.Genet.236. 331-340 (1993)

[Publications] Yamaguchi‐Shinozaki,K.: "Arabidopsis DNA encoding two desiccation‐responsive rd29 genes^<1.>" Plant Physiol.101. 1119‐1120 (1993)

[Publications] Kiyosue,T.: "Characterization of two cDNAs(ERD11 and ERD13)for dehydration‐inducible genes that encode putative glutathione S‐transferases in Arabidopsis thaliana L." FEBS Letters. 335. 189-192 (1993)

[Publications] Yamaguchi‐Shinozaki,K.: "The plant hormone abscisic acid mediates the drought‐induced expression but not the seed‐specific expression of rd22,a gene responsive to dehydration stress in Arabidopsis thaliana." Mol.Gen.Genet.238. 17-25 (1993)

[Publications] Urao,T.: "An Arabidopsis myb homolog is induced by dehydration stress and its gene product binds to the conserved MYB recognition sequence." Plant Cell. 5. 1529-1539 (1993)

[Publications] Kiyosue,T.: "Characterization of cDNA for a dehydration‐inducible gene that encodes a clp A,B‐like protein in Arabidopsis thaliana L." Biochem.Biophys.Res.Commun.196. 1214-1220 (1993)

[Publications] Koizumi,M.: "Structure and expression of two genes that encode distinct drought‐inducible cysteine protcinascs in Arabidopsis thaliana." Gene. 129. 175-182 (1993)

[Publications] Mi,H.: "Electron donation form cyclic and respiratory flows to photosynthetic intersystem chain is mediated by pyridine nucleotid dehydrogenase in the cyanobacterium Synechocy,stis PCC6803." Plant Cell Physiol.33. 1233-1237 (1993)

[Publications] Ogawa,T.: "Isolation.and characterization of the ccmM gene required by the cyanobacterium Synechocystis PCC6803 for inorganic carbon utilization" Photosyn.Res.in press. (1993)

[Publications] Ogawa,T.: "Cloning of a gene(ccmA)encoding a carboxysomal constituent of Synechocystis PCC6803." J.Bacteriol.in press. (1993)

[Publications] Mi,H.: "NAD(P)H dehydrogenase‐dependent cyclic electron flow around photosystem I in the cyanobacterium Synechocystis PCC6803:A study of dark‐starved cells and spheroplasts." Plant Cell Physiol.in press. (1993)

[Publications] Wada,M.: "Molecular cloning of P‐type ATPase from marine alga Heterosigma akashiwo." Plant Mol.Biol.in press. (1993)

村田紀夫岡崎国立共同研究機構, 基礎生物学研究所, 教授 (90011569)