1994 Fiscal Year Annual Research Report

光合成の環境適応の分子機構

Research Project

Project/Area Number	04273103
Research Institution	Okazaki National Research Institutes
Principal Investigator	村田紀夫岡崎国立共同研究機構, 基礎生物学研究所, 教授 (90011569)
Co-Investigator(Kenkyū-buntansha)	篠崎一雄理化学研究所, 主任研究員 (20124216) 藤伊正筑波大学, 生物科学系, 教授 (20011611) 杉山達夫名古屋大学, 農学部, 教授 (50023453) 水野猛名古屋大学, 農学部, 教授 (10174038) 和田敬四郎金沢大学, 理学部, 教授 (70028174)
Keywords	環境適応 / 低温 / 塩ストレス / 乾燥 / 無機イオン欠乏 / ラン藻 / 環境耐性 / 光合成
Research Abstract	研究の目的:光合成の効率に影響を与える環境要因としては、温度、塩、無機イオン欠乏などが挙げられるが、これまでの研究により、上記の環境因子への適応に関する多数の遺伝子が単離された。本年度は、これらの遺伝子を他の植物・藻類・シアノバクテリアに導入し、さらに作成された形質転換植物の表現型を解析することを目的とした。主要研究成果:(1)低温適応・耐性:不飽和化酵素の遺伝子を順次破壊した形質転換シアノバクテリアを用いて、膜脂質の不飽和化が光化学系II複合体の光失活からの回復を促進することを明らかにした。(2)塩耐性:塩耐性に関与するペタインの合成系の遺伝子(大腸菌由来)をシアノバクテリアに導入し、シアノバクテリアを塩耐性にすることができた。(3)乾燥による遺伝子発現:乾燥によって誘導されるシロイヌナズナの遺伝子rd29Aのプロモーター領域を解析し、乾燥によって直接制御を受けるシス領域とアブサイシン酸を介して制御を受けるシス領域を同定した。(4)シアノバクテリアからP・型ATPaseの遺伝子をクローニングした。その遺伝子産物はチラコイド膜に局在し、銅イオンの輸送に関与していた。P・型ATPaseの遺伝子をもう一種類クローニングしたが、その遺伝子産物はカルシウムの輸送に関与していた。

Research Products
(22 results)

All Other

All Publications (22 results)

[Publications] Gombos,Z.: "The unsaturation of membrane lipids stabilizes photosynthesis against heat stress." Plant Physiol.104. 563-567 (1994)
[Publications] Sakamoto,T.: "Identification of conserved domains in the Δ12 desaturase of cyanobacteria." Plant Mol.Biol.24. 643-650 (1994)
[Publications] Wada,H.: "Contribution of membrane lipids to the ability of the photosynthetic mechinery to tolerate temperature stress." Proc.Natl.Acad.Sci.USA. 91. 4273-4277 (1994)
[Publications] Gombos,Z.: "The recovery of photosynthesis from low-temperature photoinhibition is accelerated by the unsaturation of membrane lipids:a mechanism of chilling tolerance." Proc.Natl.Acad.Sci.USA. 91. 8787-8791 (1994)
[Publications] Sakamoto,T.: "Cloning of ω3 desaturase from cyanobacteria and its in altering the degree of membrane-lipid unsaturation." Plant Mol.Biol.26. 249-264 (1994)
[Publications] Sakamoto,T.: "Δ9 acyl-lipid desaturase of cyanobacteria:molecular cloning and substrate specificities in terms of fatty acids,sn-positions,and polar head groups." J.Biol.Chem.269. 25576-25580 (1994)
[Publications] Kiyosue,T.: "Characterization of an Arabidopsis cDNA for a soluble epoxide hydrolase gene that is inducible by auxin and water stress." Plant J.6. 259-269 (1994)
[Publications] Yosiba,Y.: "Correlation between the induction of a gene for Δ^1-pyrroline-5-caboxylate synthetase and the accumulation of proline in Arabidopsis thaliana under osmotic stress." Plant J.,in press.
[Publications] Murota,K.: "Salt tolerance in thylakoid membranes of photoautotrophically cultured green tobacco cells." Plant and Cell Physiol.35. 107-113 (1994)
[Publications] Taniguchi,M.: "Transcriptional and post-transcriptional regulation of nitrogen-responding expression of phosphoenolpyruvate carboxylase gene in maize." Plant Physiol.105. 1223-1229 (1994)
[Publications] Taniguchi,M.: "Structure of genes that encode isozymes of aspartate aminotransferase in panicum miliaceum L.,a C4 plant." Plant Mol.Biol.26. 723-734 (1994)
[Publications] Taniguchi,M.: "Aspartate aminotransferase isozymes in Panicum miliaceum L.,an NAD-malic enzyme-type C4 plant comparison of enzymatic properties,primary structures,and expression patterns." Arch.Biochem.Biophys. (in press).
[Publications] Kishitani,S.: "Accumulation of glycinebetaine during cold acclimation and freezing tolerance in leaves of water and spring barley plants." Plant,Cell Environ.17. 89-95 (1994)
[Publications] Ishitani,M.: "Gene expression of barley betaine aldehyde dehydrogenase in response to osmotic stress and ABA." Plant Mol.Biol.,in press.
[Publications] Nomura,M.: "Synechococcus sp.PCC7942 transformed with Esherichia colibet genes produces betaine from choline and acquires resistance to salt-stress." Plant Physiol.,in press.
[Publications] Nagaya,M.: "The sphR product,a two-component systems response regulator protein,regulates phosphate assimilation in Synechococcus species PCC7942 by binding to two sites upstream from the phoA promoter." J.Bacteriol.176. 2210-2215 (1994)
[Publications] Aiba,H.: "A novel gene whose expression is regulated by the response regulator,SphR,in response to phosphate limitation in Synechococcus species PCC7942." Mol.Microbiol.13. 25-34 (1994)
[Publications] Yamaguchi-Shinozaki,K.: "A novel cis-acting element in an Arabidopsis gene in involved in responsiveness to drought,low temperature,or high-salt stress." Plant Cell. 6. 251-264 (1994)
[Publications] Ogawa,T.: "Isolation and characterization of the ccmM gene required by the cyanobacterium Synechocystis PCC6803 for inorganic carbon utilization." Photosyn.Res.39. 183-190 (1994)
[Publications] Ogawa,T.: "A gene(ccmA)required for carboxysome formation in the cyanobacterium Synechocystis sp.strain PCC6803." J.Bacteriol.176. 2374-2378 (1994)
[Publications] Wada,M.: "Molecular cloning of P-type ATPase on intracellular membranes of the marine alga Heterosigma akashiwo." Plant Mol.Biol.26. 699-708 (1994)
[Publications] Ookura,T.: "Identification and characterization of a plasma membrane H^+-ATPase gene family in Orywa sativa L." Plant Cell Physiol. (in press).

1994 Fiscal Year Annual Research Report

光合成の環境適応の分子機構

Principal Investigator

村田 紀夫 岡崎国立共同研究機構, 基礎生物学研究所, 教授 (90011569)

Research Products

[Publications] Gombos,Z.: "The unsaturation of membrane lipids stabilizes photosynthesis against heat stress." Plant Physiol.104. 563-567 (1994)

[Publications] Sakamoto,T.: "Identification of conserved domains in the Δ12 desaturase of cyanobacteria." Plant Mol.Biol.24. 643-650 (1994)

[Publications] Wada,H.: "Contribution of membrane lipids to the ability of the photosynthetic mechinery to tolerate temperature stress." Proc.Natl.Acad.Sci.USA. 91. 4273-4277 (1994)

[Publications] Gombos,Z.: "The recovery of photosynthesis from low-temperature photoinhibition is accelerated by the unsaturation of membrane lipids:a mechanism of chilling tolerance." Proc.Natl.Acad.Sci.USA. 91. 8787-8791 (1994)

[Publications] Sakamoto,T.: "Cloning of ω3 desaturase from cyanobacteria and its in altering the degree of membrane-lipid unsaturation." Plant Mol.Biol.26. 249-264 (1994)

[Publications] Sakamoto,T.: "Δ9 acyl-lipid desaturase of cyanobacteria:molecular cloning and substrate specificities in terms of fatty acids,sn-positions,and polar head groups." J.Biol.Chem.269. 25576-25580 (1994)

[Publications] Kiyosue,T.: "Characterization of an Arabidopsis cDNA for a soluble epoxide hydrolase gene that is inducible by auxin and water stress." Plant J.6. 259-269 (1994)

[Publications] Yosiba,Y.: "Correlation between the induction of a gene for Δ^1-pyrroline-5-caboxylate synthetase and the accumulation of proline in Arabidopsis thaliana under osmotic stress." Plant J.,in press.

[Publications] Murota,K.: "Salt tolerance in thylakoid membranes of photoautotrophically cultured green tobacco cells." Plant and Cell Physiol.35. 107-113 (1994)

[Publications] Taniguchi,M.: "Transcriptional and post-transcriptional regulation of nitrogen-responding expression of phosphoenolpyruvate carboxylase gene in maize." Plant Physiol.105. 1223-1229 (1994)

[Publications] Taniguchi,M.: "Structure of genes that encode isozymes of aspartate aminotransferase in panicum miliaceum L.,a C4 plant." Plant Mol.Biol.26. 723-734 (1994)

[Publications] Taniguchi,M.: "Aspartate aminotransferase isozymes in Panicum miliaceum L.,an NAD-malic enzyme-type C4 plant comparison of enzymatic properties,primary structures,and expression patterns." Arch.Biochem.Biophys. (in press).

[Publications] Kishitani,S.: "Accumulation of glycinebetaine during cold acclimation and freezing tolerance in leaves of water and spring barley plants." Plant,Cell Environ.17. 89-95 (1994)

[Publications] Ishitani,M.: "Gene expression of barley betaine aldehyde dehydrogenase in response to osmotic stress and ABA." Plant Mol.Biol.,in press.

[Publications] Nomura,M.: "Synechococcus sp.PCC7942 transformed with Esherichia colibet genes produces betaine from choline and acquires resistance to salt-stress." Plant Physiol.,in press.

[Publications] Nagaya,M.: "The sphR product,a two-component systems response regulator protein,regulates phosphate assimilation in Synechococcus species PCC7942 by binding to two sites upstream from the phoA promoter." J.Bacteriol.176. 2210-2215 (1994)

[Publications] Aiba,H.: "A novel gene whose expression is regulated by the response regulator,SphR,in response to phosphate limitation in Synechococcus species PCC7942." Mol.Microbiol.13. 25-34 (1994)

[Publications] Yamaguchi-Shinozaki,K.: "A novel cis-acting element in an Arabidopsis gene in involved in responsiveness to drought,low temperature,or high-salt stress." Plant Cell. 6. 251-264 (1994)

[Publications] Ogawa,T.: "Isolation and characterization of the ccmM gene required by the cyanobacterium Synechocystis PCC6803 for inorganic carbon utilization." Photosyn.Res.39. 183-190 (1994)

[Publications] Ogawa,T.: "A gene(ccmA)required for carboxysome formation in the cyanobacterium Synechocystis sp.strain PCC6803." J.Bacteriol.176. 2374-2378 (1994)

[Publications] Wada,M.: "Molecular cloning of P-type ATPase on intracellular membranes of the marine alga Heterosigma akashiwo." Plant Mol.Biol.26. 699-708 (1994)

[Publications] Ookura,T.: "Identification and characterization of a plasma membrane H^+-ATPase gene family in Orywa sativa L." Plant Cell Physiol. (in press).

村田紀夫岡崎国立共同研究機構, 基礎生物学研究所, 教授 (90011569)