1995 Fiscal Year Annual Research Report

光合成の環境適応の分子機構

Research Project

Project/Area Number	04273103
Research Institution	Okazaki National Research Institutes
Principal Investigator	村田紀夫岡崎国立共同研究機構, 基礎生物学研究所, 教授 (90011569)
Co-Investigator(Kenkyū-buntansha)	篠崎一雄理化学研究所, 主任研究員 (20124216) 小川晃男名古屋大学, 生物分子応答研究センター, 教授 (80087593) 杉山達夫名古屋大学, 農学部, 教授 (50023453) 水野猛名古屋大学, 農学部, 教授 (10174038) 和田敬四郎金沢大学, 理学部, 教授 (70028174)
Keywords	環境適応 / 低温 / 塩ストレス / 乾燥 / 無機イオン欠乏 / ラン藻 / 環境耐性 / 光合成
Research Abstract	研究の目的:光合成の効率に影響を与える環境要因としては、温度、塩、無機イオン欠乏などが挙げられるが、これまでの研究により、上記の環境因子への適応に関する多数の遺伝子が単離された。本年度は、これらの遺伝子を他の植物・藻類・シアノバクテリアに導入し、さらに作成された形質転換植物の表現型を解析することを目的とした。主要研究成果:(1)低温適応・耐性:不飽和化酵素の遺伝子を順次破壊した形質転換シアノバクテリアを用いて、膜脂質の不飽和化が光化学系II複合体の光失活に伴うD1タンパク質再成を促進することを明らかにした。(2)塩耐性:塩耐性に関与するベタインを合成するコリンオキシダーゼの遺伝子(土壌細菌由来)をシアノバクテリアとシロイヌナズナに導入し、これらの生物を塩耐性ならびに低温耐性にすることができた。(3)乾燥と低温:シロイヌナズナのホスホイノシトールに特異的なホスファターゼCの遺伝子の発現は、乾燥と低温のいずれによっても誘導されることを明らかにした。(4)CO_2濃縮機構:CO_2濃縮能を欠損したシアノバクテリアを作成し、その相補を利用してCO_2濃縮酵素の遺伝子を単離した。

Research Products

(16 results)

All Other

All Publications (16 results)

[Publications] Murata,N.: "Acyl-lipid desaturases and their importance in the tolerance and acclimation to cold of cyanobacteria." Biochemical Journal. 308. 1-8 (1995)
[Publications] Moon,B.Y.: "Unsaturation of chloroplastic membrane lipids protects photosynthesis against low-temperature photoinhibition in transgenic tobacco plants." Proceedings of the National Academy of Sciences of the United State of America. 92. 6219-6223 (1995)
[Publications] Deshnium,P.: "Transformation of Synechococcus with a gene for choline oxidase enhances tolerance to salt stress." Plant Molecular Biology. 29. 897-907 (1995)
[Publications] Sato,N.: "A family of cold-regulated RNA-binding protein genes in the cyanobacterium Anabaena variabilis M3." Nucleic Acids Research. 23. 2161-2167 (1995)
[Publications] Sanada,Y.: "Novel light-dark change of proline levels in Halophyte(Mesembryanthemum crystallinum L.)and Glycophytes(Hordeum vulgare L.and Triticum aestivum L.)leaves and roots under salt stress." Plant and Cell Physiology. 36. 965-970 (1995)
[Publications] Yoshiba,Y.: "Correlation between the induction of a gene for^1-pyrroline-5-carboxylate synthetase and the accumulation of proline in Arabidopsis thaliana under osmotic stress." Plant Journal. 7. 751-760 (1995)
[Publications] Ishitani,M.: "Expression of the betaine aldehyde dehydrogenase gene in barley in response to osmotic stress and abscisic acid." Plant Molecular Biology. 27. 307-315 (1995)
[Publications] Nomura,M.: "Synechococcus sp.PCC7942 transformed with Escherichia coli bet genes produces glycine betaine from choline and acquires resistance to salt stress." Plant Physiology. 107. 703-708 (1995)
[Publications] Kashiwagi,S.: "A Synechococcus gene encoding a putative pore-forming intrinsic membrane protein." Biochimica et Biophysica Acta. 1237. 189-192 (1995)
[Publications] Hirayama,T.: "A gene encoding a phosphatidylinositol-specific phospholipase Cis induced by dehydration and salt stress in Arabidopsis thaliana." Proceedings of the National Academy of Sciences of the United State of America. 92. 3903-3907 (1995)
[Publications] Mizoguchi,T.: "Two genes that encode ribosomal S6 Kinase homologs are induced by cold or salinity stress in Arabidopsis thaliana." FEBS Letters. 358. 199-204 (1995)
[Publications] Sato,F.: "Synthesis and secretion of tobacco neutral PR-5 protein by transgenic tobacco and yeast." Biochemical and Biophysical Research Communications. 211. 909-913 (1995)
[Publications] Sakakibara,H.: "Isolation and characterization of a cDNA that encodes maize glutamate dehydrogenase." Plant and Cell Physiology. 36. 789-797 (1995)
[Publications] Taniguchi,M.: "Aspartate aminotransferase isozymes in Panicum miliaceum L.,an NAD-malic enzyme-the C_4 plant:comparison of enzymatic properties,primary structures,and expression patterns." Archives of Biochemistry and Biophysics. 318. 295-306 (1995)
[Publications] Mi,H.: "Thylakoid membrane-bound,NADPH-specific pyridine nucleotide dehydrogenase complex mediates cyclic electron transport in the cyanobacterium Synechocystis sp.PCC6803." Plant and Cell Physiology. 36. 661-668 (1995)
[Publications] Shono,M.: "Partial purification of a Na^+-pump in the plasma membrane of marine alga Heterosigma akashiwo." Plant Physiology. 108. 1615-1621 (1995)

1995 Fiscal Year Annual Research Report

光合成の環境適応の分子機構

Principal Investigator

村田 紀夫 岡崎国立共同研究機構, 基礎生物学研究所, 教授 (90011569)

Research Products

[Publications] Murata,N.: "Acyl-lipid desaturases and their importance in the tolerance and acclimation to cold of cyanobacteria." Biochemical Journal. 308. 1-8 (1995)

[Publications] Moon,B.Y.: "Unsaturation of chloroplastic membrane lipids protects photosynthesis against low-temperature photoinhibition in transgenic tobacco plants." Proceedings of the National Academy of Sciences of the United State of America. 92. 6219-6223 (1995)

[Publications] Deshnium,P.: "Transformation of Synechococcus with a gene for choline oxidase enhances tolerance to salt stress." Plant Molecular Biology. 29. 897-907 (1995)

[Publications] Sato,N.: "A family of cold-regulated RNA-binding protein genes in the cyanobacterium Anabaena variabilis M3." Nucleic Acids Research. 23. 2161-2167 (1995)

[Publications] Sanada,Y.: "Novel light-dark change of proline levels in Halophyte(Mesembryanthemum crystallinum L.)and Glycophytes(Hordeum vulgare L.and Triticum aestivum L.)leaves and roots under salt stress." Plant and Cell Physiology. 36. 965-970 (1995)

[Publications] Yoshiba,Y.: "Correlation between the induction of a gene for^1-pyrroline-5-carboxylate synthetase and the accumulation of proline in Arabidopsis thaliana under osmotic stress." Plant Journal. 7. 751-760 (1995)

[Publications] Ishitani,M.: "Expression of the betaine aldehyde dehydrogenase gene in barley in response to osmotic stress and abscisic acid." Plant Molecular Biology. 27. 307-315 (1995)

[Publications] Nomura,M.: "Synechococcus sp.PCC7942 transformed with Escherichia coli bet genes produces glycine betaine from choline and acquires resistance to salt stress." Plant Physiology. 107. 703-708 (1995)

[Publications] Kashiwagi,S.: "A Synechococcus gene encoding a putative pore-forming intrinsic membrane protein." Biochimica et Biophysica Acta. 1237. 189-192 (1995)

[Publications] Hirayama,T.: "A gene encoding a phosphatidylinositol-specific phospholipase Cis induced by dehydration and salt stress in Arabidopsis thaliana." Proceedings of the National Academy of Sciences of the United State of America. 92. 3903-3907 (1995)

[Publications] Mizoguchi,T.: "Two genes that encode ribosomal S6 Kinase homologs are induced by cold or salinity stress in Arabidopsis thaliana." FEBS Letters. 358. 199-204 (1995)

[Publications] Sato,F.: "Synthesis and secretion of tobacco neutral PR-5 protein by transgenic tobacco and yeast." Biochemical and Biophysical Research Communications. 211. 909-913 (1995)

[Publications] Sakakibara,H.: "Isolation and characterization of a cDNA that encodes maize glutamate dehydrogenase." Plant and Cell Physiology. 36. 789-797 (1995)

[Publications] Taniguchi,M.: "Aspartate aminotransferase isozymes in Panicum miliaceum L.,an NAD-malic enzyme-the C_4 plant:comparison of enzymatic properties,primary structures,and expression patterns." Archives of Biochemistry and Biophysics. 318. 295-306 (1995)

[Publications] Mi,H.: "Thylakoid membrane-bound,NADPH-specific pyridine nucleotide dehydrogenase complex mediates cyclic electron transport in the cyanobacterium Synechocystis sp.PCC6803." Plant and Cell Physiology. 36. 661-668 (1995)

[Publications] Shono,M.: "Partial purification of a Na^+-pump in the plasma membrane of marine alga Heterosigma akashiwo." Plant Physiology. 108. 1615-1621 (1995)

村田紀夫岡崎国立共同研究機構, 基礎生物学研究所, 教授 (90011569)