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1996 Fiscal Year Final Research Report Summary

Molecular Mechanisms for Responses of Photosynthetic organisms to Environments

Research Project

Project/Area Number 04273103
Research Category

Grant-in-Aid for Scientific Research on Priority Areas

Allocation TypeSingle-year Grants
Research InstitutionOkazaki National Research Institutes

Principal Investigator

MURATA Norio  National Institute for Basic Biology, Professor, 基礎生物学研究所, 教授 (90011569)

Co-Investigator(Kenkyū-buntansha) SHINOZAKI Kazuo  Institute of Physical and Chemical Research, Chief Researcher, 主任研究員 (20124216)
OGAWA Teruo  Nagoya University, Professor, 生物分子応答研究センター, 教授 (80087593)
SUGIYAMA Tatsuo  Nagoya University, Professor, 農学部, 教授 (50023453)
MIZUNO Takeshi  Nagoya University, Professor, 農学部, 教授 (10174038)
WADA Keishiro  Kanazawa University, Professor, 理学部, 教授 (70028174)
Project Period (FY) 1992 – 1995
KeywordsEnvironment / Acclimation / Tolerance / Photosynthesis / Low temperature / Salt stress / Ion deficiency / Cyanobacteria
Research Abstract

Purpose of Research : The purpose of this research was ; (1) to isolate genes which are involved in the acclimation of photosynthetic organisms to varieties of environmental conditions ; (2) to transform higher plants and cyanobacteria with the isolated genes ; and (3) to elucidate the molecular mechanisms of the acclimation to the environment by analyzing the phenotypes of transformed plants.
Main Results of Research : (1) Acclimation and tolerance to low temperature : we isolated, from cyanobacteria, several genes for fatty acid desaturases which are involved in the acclimation of photosynthetic organisms to low temperature. Analysis of mutants in which these genes were inactivated by insertion of antibiotic gene cartridges, indicated that the unsaturation of fatty acids of membrane lipids protected the photosystem II complex against photoinhibition by accelerating the biogenesis of the D1 protein. (2) Tolerance to salt stress : we isolated, from a soil bacterium, the codA gene for ch … More oline oxidase that synthesizes glycinebetaine which is involved in the acclimation to and tolerance of photosynthetic organisms to the salt stress. The transformation of cyanobacteria and Arabidopsis thaliana with the codA gene enhanced the tolerance to salt stress by protection of the photosystem II complex from the photoinhibited state. (3) Mechanisms of concentration of CO_2 ; cyanobacterial cells actively accumulate CO_2 when they are exposed to low-CO_2 conditions. We isolated a mutant which was defective in the ability ot intracellularly concentrate CO_2 and isolated a gene for the ability by complementation. (4) Acclimation to deficiency of inorganic ions : We isolated a gene for a sensor of deficiency of phosphate and a gene for a transcription factor which was phosphorylated under control of the sensor. (5) Drought-induced gene expression : One of the drought-induced genes in Arabidopsis thaliana is rd29A.Effects of systematic deletions of the upstream region of this gene indicated that there are two cis-regions ; one is regulated directly by drought and the other via synthesis of abscisic acid. Less

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] N.Murata: "Genetically engineered alteration in the chilling sensitivity of plants." Nature. 356. 710-713 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Ogawa: "cemA homologue essential to CO_2 transport in the cyanobacterium Synechocystis PCC6803" Proceeding of National Academy of Sciences U.S.A.93. 4006-4010 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Mizuno: "Sensor and regular proteins from the cyanobacterium Synechococcus species PCC7942 that belong to the Bacterial signal-transduction protein families:implication in the adaptive response to phosphate limitation" Molucular Microbiology. 8. 81-91 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Tasaka: "Targeted mutagenesis of acyl-lipid desaturases in Synechocystis:Evidence for the important roles of polyunsaturated membrane lipids in growth,respiration and photosynthesis." EMBO Journal. 15. 6416-6425 (1996)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Yamaguchi-Shinozaki: "Arabidopsis DNA encoding two desication-responsive rd29 genes." Plant Physiology. 101. 1119-1120 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Hayashi: "Transformation of Arabidopsis thaliana with the codA gene for choline oxidase;accumulation of glycinebetaine and enhanced tolerance to salt and cold stress" Plant Journal. (in press). (1997)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] N.Murata: "Biochemistry and Motecular Biology of Membrane and Storage Lipids of Plants." The American Society of Plant Physiologists, 259 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] N.Murata: "Research in Photosynthesis" Kluwer Academic Publishers, 3400 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] N.Murata: "Genetically engineered alteration in the chilling sensitivity of plants." Nature. 356. 710-713 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Ogawa: "CemA homologue essential to CO2 transport the cyanobacterium Synechocystis PCC6803." Proceeding of National Academy of Sciences U.S.A.93. 4006-4010 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Mizuno: "Sensor and regular proteins from the cyanobacterium Synechococcus species PCC7942 that belong to the bacterial signal-transduction protein families : implication in the adaptive response to phosphate limitation." Molucular Microbiology. 8. 81-911 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Tasaka: "Targeted mutagenesis of acyl-lipid desaturases in Synechocystis : Evidence for the important roles of polyunsaturated membrane lipids in growth, respiration and photosynthesis." EMBO Journal. 15. 6416-6425 (1996)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Yamaguchi-Shinozaki: "Arabidopsis DNA encoding two desication-responsive rd29 genes." Plant Physiology. 101. 1119-1120 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Hayashi: "Transformation of Arabidopsis thaliana with the codA gene for choline oxidase ; accumulation of glycinebetaine and enhanced tolerance to salt and cold stress" Plant Journal. (in press). (1997)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] N.Murata (ed.): Biochemistry and Molecular Biology of Membrane and Storage Plants.The American Society of Plan Lipids of Physiologists, 259 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] N.Murata (ed.): Research in Photosynthesis. Kluwer Academic Publishers, 3400 (1992)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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