1993 Fiscal Year Annual Research Report

レセプターチャンネル間シグナル伝達のクロストーク

Research Project

Project/Area Number	04304028
Research Institution	KYUSYU UNIVERSITY
Principal Investigator	赤池紀生九州大学, 医学部, 教授 (30040182)
Co-Investigator(Kenkyū-buntansha)	清原壽一京都工芸繊維大学, 繊維学部, 教授 (50071874) 北里宏滋賀医科大学, 医学部, 教授 (20079700) 岡田泰伸岡崎国立共同研究機構, 生理学研究所, 教授 (10025661) 井上勲徳島大学酸素科学研究センター, 助教授 (80001973) 赤須崇久留米大学, 医学部, 教授 (60113213)
Keywords	レセプター / イオンチャネル / セカンドメッセンジャー / クロストーク / 膜脂質代謝 / 細胞内カルシウム / 蛋白リン酸化
Research Abstract	今年度はまず平成4年度に得られた細胞内および細胞外の各種入力信号に応答するイオンチャンネルの分類と生理的役割の検討を以下のごとく行った。自律神経節細胞のMチャンネル(赤須、小林)、中枢神経細胞のイオノトロピックおよびメタボトロピック受容体刺激の電気的膜特性とそれに関連する細胞内2次メッセンジャー系の同定ならびに各受容器間の相互作用(赤池)、電位依存性Naチャンネル解放物質の構造特性(瀬山)、血管内皮細胞の内因性Kチャンネル活性化物質の生理的役割(鈴木)、平滑筋(富田)及び心筋(野間)細胞のイオン電流に対する物理的修飾機構、体温調節中枢の温度感受性ニューロン(清原)、小腸上皮細胞のメカノトランスダクションチャンネル(岡田)、イカ巨大神経軸索のClチャンネル(山岸)の電気生理学的分類と細胞内制御機構の同定、ミトコンドリア膜電位勾配の制御機構(北里)、細胞内Caシグナルのモニタリングとその放出・流入制御機構(井上,九場、反町)、神経分化の細胞内制御(高橋)、ギャップ結合の形成過程(外崎)、レセプターを介する軸索輸送の制御(竹中)。研究計画ではその上でさらに2次メッセンジャーを介したチャンネル・チャンネル間共同現象の発現メカニズムを解明することにしていたが、結果は必ずしも十分ではなかった。その原因は電気生理学的測定法の難しさにあると言って良い。たとえばイオンチャンネルの開閉を視覚的に正確に記録するためにはパッチクランプ法が不可欠であるが、それでは細胞内の2次メッセンジャー系は破壊されてしまう。ニスタチンはその有効な手助けとなるが、イオンチャネル開閉の解析はノイズ解析を用いざるを得なくなる。本研究の研究分担者はそれぞれの分野で日本を代表する専門家であり、今後ともさらに密に連絡を取り合い、問題点の克服と技術の普及に努める必要がある。

Research Products

(18 results)

All Other

All Publications (18 results)

[Publications] Takenaka,T.: "Effects of neurotransmitters on axoplasmic trnsport;achetylcholine effect on superior cervical ganglion cells" Brain Res.588. 212-216 (1992)
[Publications] Katori,Y.: "Fine structure of apical surface of lamprey neuromast" Sendai Sympo.Chochlear Neurobiol.2. 47-52 (1992)
[Publications] Okashiro,T.: "Effects of intracellular ATP on calcium current in freshly dispersed single cells of gunea-pig portal vein" Exp.Physiol.77. 719-731 (1992)
[Publications] Mitsuie,T.: "Exponential activation of the cardiac Na_+ current in single guinea pig ventricu-late cells" J.Physiol. 453. 261-277 (1992)
[Publications] Sugita,S.: "Calsium antagonists protect guinea-pig hippocampal CAl neurons from the irreversible change induced by elevation of the external potassium concentration" Adv.Neurotrauma Res.4. 21-26 (1992)
[Publications] Enomoto,K.: "Mechanically induced electrical and intracellular calcium in normal and cancerou mammary cells" Cell Calcium. 13. 501-511 (1992)
[Publications] Ebihara,S.: "Thyrotropin-releasing hormone operated potassium currents in dissociated CAl pyramidal neurones of rat hippocampus" J.Physiol.472. 689-710 (1993)
[Publications] Shoji,S.: "Presynaptic facilitation of excitatory postsynaptic potential by glucagon in neurons of rat ventromedial hypothalamic slices" Neurosci.Le t.138. 291-294 (1992)
[Publications] Amar,M.: "Patch clamp analysis of the effect of the insectide deltamethrin on insect neurones" J.Exp.Biol.163. 65-84 (1992)
[Publications] Sakai,Y.: "Arachidonic acid and prostaglandin E#2# activate small-conductance Cl^- channels in the basolateral membrane of rabbit parietal cell" J.Physiol.448. 293-306 (1992)
[Publications] Omatsu-Kanbe,M.: "Insulin and noradrenaline independently stimulate the translocation of glucose transporters from intracellular stores to the plasma membrane in mouse brown adipocyt" FEBS LETTERS. 314. 246-250 (1992)
[Publications] T.MATSUDA.: "Thermal and RGE#2# sensitivity of the organum vasculosum lamina terminalis region and preoptic area in the rat brain slices" J.Physiol.454. 197-212 (1992)
[Publications] Nohmi M.: "Basal Ca2#+# and the oscillation of Ca2#2# in caffeine-treated bullfrog sympathetic neurones" J.Physiol.450. 513-528 (1992)
[Publications] Kobayashi,H.: "Intracellular transduction mechanisms for the slow synaptic events" Can.J.Physiol.Pharmacol.70. S44-50 (1992)
[Publications] Suzuki,H.: "Nitroarginine-sensitive and insensitive components of the endothelium-dependent relaxation in the guinea-pig carotid artery" Jpn.J.Physiol.42. 335-347 (1992)
[Publications] Iwasa,J.: "Synthesis of the photoaffinity-labeled grayanotoxins for studying the gating mechanism of Na channel" Biosci.Biotech.Biochem.56. 1169-1171 (1992)
[Publications] Sorimachi,M.: "A muscarinic receptor agonist mobilizes Ca2#+# from caffeine and inositol-1,4,5-trisphosphate-sensitive stores in cat adrenal chromafin cells" Brain Res.571. 154-158 (1992)
[Publications] Takahashi,K.: "Cellular mechanism of neuron-spesific expression of Na#+# channels in the two interacting cleavage-arrested embryonic cells isolated from an ascidian embryo" Cell Structure and Function. 18 (in press). (1994)

1993 Fiscal Year Annual Research Report

レセプターチャンネル間シグナル伝達のクロストーク

Principal Investigator

赤池 紀生 九州大学, 医学部, 教授 (30040182)

Research Products

[Publications] Takenaka,T.: "Effects of neurotransmitters on axoplasmic trnsport;achetylcholine effect on superior cervical ganglion cells" Brain Res.588. 212-216 (1992)

[Publications] Katori,Y.: "Fine structure of apical surface of lamprey neuromast" Sendai Sympo.Chochlear Neurobiol.2. 47-52 (1992)

[Publications] Okashiro,T.: "Effects of intracellular ATP on calcium current in freshly dispersed single cells of gunea-pig portal vein" Exp.Physiol.77. 719-731 (1992)

[Publications] Mitsuie,T.: "Exponential activation of the cardiac Na_+ current in single guinea pig ventricu-late cells" J.Physiol. 453. 261-277 (1992)

[Publications] Sugita,S.: "Calsium antagonists protect guinea-pig hippocampal CAl neurons from the irreversible change induced by elevation of the external potassium concentration" Adv.Neurotrauma Res.4. 21-26 (1992)

[Publications] Enomoto,K.: "Mechanically induced electrical and intracellular calcium in normal and cancerou mammary cells" Cell Calcium. 13. 501-511 (1992)

[Publications] Ebihara,S.: "Thyrotropin-releasing hormone operated potassium currents in dissociated CAl pyramidal neurones of rat hippocampus" J.Physiol.472. 689-710 (1993)

[Publications] Shoji,S.: "Presynaptic facilitation of excitatory postsynaptic potential by glucagon in neurons of rat ventromedial hypothalamic slices" Neurosci.Le t.138. 291-294 (1992)

[Publications] Amar,M.: "Patch clamp analysis of the effect of the insectide deltamethrin on insect neurones" J.Exp.Biol.163. 65-84 (1992)

[Publications] Sakai,Y.: "Arachidonic acid and prostaglandin E#2# activate small-conductance Cl^- channels in the basolateral membrane of rabbit parietal cell" J.Physiol.448. 293-306 (1992)

[Publications] Omatsu-Kanbe,M.: "Insulin and noradrenaline independently stimulate the translocation of glucose transporters from intracellular stores to the plasma membrane in mouse brown adipocyt" FEBS LETTERS. 314. 246-250 (1992)

[Publications] T.MATSUDA.: "Thermal and RGE#2# sensitivity of the organum vasculosum lamina terminalis region and preoptic area in the rat brain slices" J.Physiol.454. 197-212 (1992)

[Publications] Nohmi M.: "Basal Ca2#+# and the oscillation of Ca2#2# in caffeine-treated bullfrog sympathetic neurones" J.Physiol.450. 513-528 (1992)

[Publications] Kobayashi,H.: "Intracellular transduction mechanisms for the slow synaptic events" Can.J.Physiol.Pharmacol.70. S44-50 (1992)

[Publications] Suzuki,H.: "Nitroarginine-sensitive and insensitive components of the endothelium-dependent relaxation in the guinea-pig carotid artery" Jpn.J.Physiol.42. 335-347 (1992)

[Publications] Iwasa,J.: "Synthesis of the photoaffinity-labeled grayanotoxins for studying the gating mechanism of Na channel" Biosci.Biotech.Biochem.56. 1169-1171 (1992)

[Publications] Sorimachi,M.: "A muscarinic receptor agonist mobilizes Ca2#+# from caffeine and inositol-1,4,5-trisphosphate-sensitive stores in cat adrenal chromafin cells" Brain Res.571. 154-158 (1992)

[Publications] Takahashi,K.: "Cellular mechanism of neuron-spesific expression of Na#+# channels in the two interacting cleavage-arrested embryonic cells isolated from an ascidian embryo" Cell Structure and Function. 18 (in press). (1994)

赤池紀生九州大学, 医学部, 教授 (30040182)