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1994 Fiscal Year Final Research Report Summary

Establishment of the pharmacological sciences to elucidate the signal transduction system.

Research Project

Project/Area Number 04304030
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field General pharmacology
Research InstitutionNagoya University

Principal Investigator

HIDAKA Hiroyoshi  Nagoya University School of Medicine, Professor, 医学部, 教授 (80100171)

Co-Investigator(Kenkyū-buntansha) SAITO Hideya  Hokkaido University School of Medicine, Professor, 医学部, 教授 (20000929)
TODA Noboru  Shiga University of Medical Science, Professor, 医学部, 教授 (50025590)
OHTSUKA Masanori  Tokyo Medical and Dental University, School of Medicine, Professor emeritus, 医学部, 名誉教授 (60013801)
KURIYAMA Kinya  Kyoto Prefectural University of Medicine, Professor, 医学部, 教授 (20079734)
ENDO Makoto  Saitama Medical School, Professor, 医学部, 教授 (50009990)
Project Period (FY) 1992 – 1994
Keywordssignal transduction / intracellular message system / molecular probe / 機能探索子
Research Abstract

One of the aims of this research project was to elucidate and control the mechanisms by which the intracellular second messenger systems transduce signals derived from extracellular stimuli, using specific molecular probes. The other was to create novel function-targeted probes which are useful to investigate the intracellular mechanisms. Such a pharmacological approach is undoubtedly powerful since various biological phenomena are controlled by a network of multiple intracellular messenger systems. Through three-year work under this grant, increasing number of specific molecular probes have became available, which enabled us to understand intracellular messenger systems. A good example is KN-62 and 93, specific inhibitors of Ca/calmodulin-dependent protein kinase II,which have been widely used to investigate the functions of the kinase. We also succeeded to purify the novel type of Ca/calmodulin-dependentkinase. V.We obtained evidence concerning signal transduction mechanisms by tachykinin, GABA and serotonin in neurons using pharmacological agents. Nitric oxide was demonstrated to be involved in the neural control of the brain vessel dilation.From an molecular biological approach, two types of Ca channels releasing Ca^<2+>from the intracellular store sites have been cloned. All these findings indicate that novel specific probes are desperately required for fundamental understanding of the intracellular messenger systems. Furthermore, these probes may provide a clue to create new compounds for a clinical use.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] H.Mochizuki: "Purification and characterization of Ca^<2+>/calmodulin-dependent protein kinase V from rat cerebrum." J.Biol.Chem.268. 9143-9147 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Oyamada: "Primary structure and distribution of ryanodine-binding protein isoforms of the bullfrog skeletal muscle." J.Biol.Chem.269. 17206-17214 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Nakayasu: "Immunoaffinity purification and characterization of γ-aminobutyric acid(GABA)_Breceptor from bovine cerebral cortex." J.Biol.Chem.268. 8658-8664 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] R.Hosoki: "Effects of RP67580,a tachykinin NK1 receptor antagonist,on a primary afferent-evoked response of ventral roots in the neonatal rat spinal cord." Br.J.Pharmacol.113. 1141-1146 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Ayajiki: "Nitric pxide mediates,and acetylcholine modulates,neurally induced relaxation of bovine cerebral arteries." Neuroscience. 54. 819-825 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Yoshioka: "Involvement of 5-hydroxytrytamin3 receptors in the initiation of pharyngeal reflex." Am.J.Physiol.266. R1652-R1658 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Mochizuki: "Purification and characterization of Ca^<2+>/calmodulin-dependentprotein kinase V from rat cerebrum." J.Biol.Chem.268. 9143-9147 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Oyamada: "Primary structure and distribution of ryanodinebinding protein isoforms of the bullfrog skeletal muscle." J.Biol.Chem.269. 17206-17214 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Nakayasu: "Immunoaffinity purification and characterization ofgamma-aminobutyric acid(GABA)_Breceptor from bovine cerebral cortex." J.Biol.Chem.268. 8658-8664 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] R.Hosoki Effects of RP67580: "a tachykinin NK1 receptor antagonist, on a primary afferent-evoked response of ventral roots in the neonatal rat spinal cord." Br.J.Pharmacol.113. 1141-1146 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Ayajiki: "Nitric pxide mediates, and acetylcholine modulates, neurally induced relaxation of bovine cerebral arteries." Neuroscience. 54. 819-825 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Yoshioka: "Involvement of 5-hydroxytrytamin3 receptrors in the initiation of pharyngeal reflex." Am.J.Physiol.266. R1652-R1658 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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