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1993 Fiscal Year Final Research Report Summary

Molecular mechanisms of bacterial toxins on host cells

Research Project

Project/Area Number 04304032
Research Category

Grant-in-Aid for Co-operative Research (A)

Allocation TypeSingle-year Grants
Research Field 細菌学
Research InstitutionJichi Medical School

Principal Investigator

NAKANO Masayasu  Jichi Medical School, Dept.Microbiol., Professor, 医学部, 教授 (70048958)

Co-Investigator(Kenkyū-buntansha) HONDA Takeshi  Osaka Univ., Research Institute for Microbial Deseases, Professor, 微生物病研究所, 教授 (60029808)
TAKEDA Yoshifumi  Kyoto University Sch.Medicine, Professor, 医学部, 教授 (30029772)
YOKOCHI Takashi  Aichi Medical Univ., Dept.Microbiol., Professor, 医学部, 教授 (20126915)
MITUYAMA Masao  Niigata University Sch.Medicine, Professor, 医学部, 教授 (10117260)
NODA Masatoshi  Chiba University School of Medicine, Professor, 医学部, 教授 (60164703)
Project Period (FY) 1992 – 1993
KeywordsBacterial toxins / Endotoxin / Lipopolysaccharide / Exotoxin / Cytotoxic toxins
Research Abstract

1. Research for intracellular toxins (endotoxins and microcystins) :
(A) LPS (endotoxins) : LPS-induced TNF-alpha production by the murine peritoneal macrophages is dependent on protein kinase Cbeta, but the production of IL-1beta depends mainly on calmodulin.adrenocorticoides participate in LPS-induced murine thymocyte apoptosis. P.gingivalis LPS activates tyrosine kinases in LPS-nonresponsive C3H/HeJ B cells. (B) Damage of rat liver cells by microcystin is partialy dependent on the cytotoxic mediators produced by non-parenchymal liver cells.
2. Research for exotoxins :
No.167 (glutamic acid) and no.170 (arginine) amino acids from N-terminal of A subunit are essential for the cytotoxic activity of Vero toxin. Protease, furin, in target cell membrane is essential for activation of diphthelia toxin. Hemolytic toxin, TDH, of V.parahemolytics phosphorylates 25kDa protein in red blood cells after binding the receptors. The enhancer (ARFs) and suppressor (ARIs) of cholera toxin were purified from target cells. The mechnisms on penetration of ST1 toxin through inner membrane of E.coil was clarified. The receptor gene for ST toxin was transfected to COS7 cells and the products were analyzed. C.difficile produces a red blood cell agglutinin which is different from toxin A.The release of neurotransmitter by tetanus toxin depends on calmodulin. The listerolysin O-producing strains of L.monocytogenes only can induce inflamatory cytokine mRNAs in infected hosts and endow them the cellular immunity. Protease SH produced by A-group streptococci may be participated in septic shock. Super-antigens may cause scalet fever, symptomes of Yersinia and Kawasaki disease.

  • Research Products

    (17 results)

All Other

All Publications (17 results)

  • [Publications] Nakano,M.他: "Intracellular protein phosphorylation in murine peritoneal macrophages in response to bacterial lipopolysaccharide(Lps)." Immunobiology. 187. 272-282 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nishikawa,T.他: "Cholera toxin directly stimulates pregnenolone generation with increasing Ca^<2+> efflux in bovine adrenocortical mitochondria" J.Steroid Biochem.Molec.Biol. 46. 203-208 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshikawa,H.他: "Membrane damage and interleukin-1 production in murine macrophages exposed to listeriolysin O" Infection and Immunity. 61. 1334-1339 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Zhang,Y-H.他: "In viro indaction of apoptosis (programmed cell death)in mouse thymus by administration of Lps" Infection and Immunity. 61. 5044-5047 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takeda,Y.他: "Vero toxins(Shiga-like toxins)produced by enterohemorrhagic Escherichia coli(Verocytotoxin-producing E.Coli)" Microbiology and Immunology. 37. 591-599 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Honda,T 他: "The pathogenicity of Vibrio parahaemolyticus and the role of the thermostable direct haemolysin and related haemolysins" Reviews in Medical Microbiology. 4. 106-113 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nakano, M.et al.: "Intracellular protein phosphorylation in murine peritoneal macrophages in response to bacteral lipopolysaccharide (LPS)" Immunobiology. 187. 272-282 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shirai, M.et al.: "Toxicity of Microcystis, Cyanobacteria : A Review (in Japanese)" Japn.J.Bacteriol.48. 511-522 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uchiyama, T.et al.: "Superantigenic properties of a noval mitogenic substance produced by Yersinia psedotuberculosis isolated from patients manifesting acute and system symptomes." J.Immunol.151. 4407-4413 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takeda, Y.et al.: "Vero toxins (Shiga-like toxins) produced by enterohemorrhagic Esherichia coli (Vero-cytotoxin-producing E.coli)." Microbiol.Immunol.37. 591-599 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishikawa, T.et al.: "Cholera toxin directly stimulates pregnenolone generation with increasing Ca^<2+> efflux in bovine adrenocortical mitochondria." J.Steroid Molec.Biol.46. 203-208 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirayama, T.et al.: "Expression of a trucated guanylate cyclase (GC-C), a receptor for heat-stable enterotoxin of enterotoxigenic Escherichia coli, and its dime formation in COS-7 cells." Microb.Pathog.15. 293-291 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Xu, M.et al.: "Demonstration and characterization of simultaneous production of a thermostable direct hemolysin (TDH/I) and a TDH-related hemolysin (TRHx) by a clinically isolated Vibrio parahemolyticus strain TH3766." Infect.Immun.62. 166-171 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matuda, M.et al.: "Tetanus toxin and Clostridium perfringens enterotoxin as tools for the study of exocytosis." Ann.N.Sci.710. 94-106 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshikawa, H.et al.: "Membrane damage and interleukin-1 production murine macrophages exposed to listeriolysin 0." Infect.Immun.61. 1334-1339 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsuneoka, M.et al.: "Evidence for involvement of furin in cleavage and activation of diphtheria toxin." J.Biol.Chem.268. 26464-26465 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Zhang, Y-H.et al.: "In vivo induction of apoptosis (programd cell death) in mouse thymus by administration of LPS." Infect.Immun.61. 5044-5048 (1993)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1995-03-27  

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