1993 Fiscal Year Annual Research Report

新しい肺保存液の開発とADF/hThioredoxineのラジカルスカベンジャー効果

Research Project

Project/Area Number	04404055
Research Institution	Kyoto University
Principal Investigator	和田洋巳京都大学, 胸部疾患研究所, 助教授 (90167205)
Co-Investigator(Kenkyū-buntansha)	横見瀬裕保京都大学, 胸部疾患研究所, 助手 (80231728)
Keywords	トレハロース / ET-Kyoto液 / ADF / Thioredoxin / 肺移植 / 虚血再灌流障害
Research Abstract	1.ユーロコリンズ液のグルコースにかわりトレハロースが加えられた液で12時間保存犬肺が良好な肺機能を示し、トレハロースの有効性が証明された(Hirata T et al.Surgery,1994)。そこで、ユーロコリンズ液イオン組成で7%或は10%トレハロースが加えられた液を用いて20時間犬肺保存後、左肺移植を行なったところ、7%トレハロースが加えられた液は10%より良好であっり、最適トレハロースの濃度は7%以下と考えられたが、それでさえ充分な肺保存効果を示さず、ユーロコリンズ液イオン組成液の限界が明らかとなった。そこで、クロライドシフト(塩素による水分子の細胞内移動)を来さない陰イオンとしてグルコネートを、膠質浸透圧源にHESを、糖質としてトレハロースを含む細胞外液型ET-K液と細胞内液型IT-K液の2種類の臓器保存液を作成し、20時間の犬肺保存実験を行った。この結果、細胞外液型ET-K液は細胞内液型IT-K液やユーロコリンズ液と比較し有意に良好なPaO2値を示し、良好な肺保存効果を認めた。 2.ADFによる肺再灌流障害の防止:大腸菌レコンビナントで作成した人サイオレドキシン(ADF:30mg/kg)を用いて、ラット肺動脈75分間クランプ実験による再灌流障害の予防もしくは減弱化を検討したところ、ADF投与群で有意にPaO2値が良好であった(H.Yokomise et al.Respiration,1994,accepted)。同様の実験を、兎、犬で行っている。 3.FK506大量投与による犬移植肺の免疫寛容導入:トロント大学と共同で行った。通常使用量の10倍を投与すると、30日間の免疫寛容があったが、それ以後は肺は拒絶された(T.Hirai et al:Ann Thoraic Surgery;1993/09 accepted)。

Research Products
(39 results)

All Other

All Publications (39 results)

[Publications] Inui.K,Schaefers.HY,Aoki.M,Wada.H,Becker.V,et al: "Effect of Methylpredonisolone and Prostacyclin on Bronchial Perfusion in Lung Transplantation" Ann Thorac Surg. 55. 464-469 (1993)
[Publications] Hasegawa.S,Yokomise.H,Wada.H,et al: "Combination use with suboptimal dosage of FK506 and Cyclosporine A in Canine lung transplantation" J Thorac Cardiovasc Surg. 104. 1340-1348 (1992)
[Publications] Hirai.T,Wada.H,Hasegawa.S,Hitomi.S,et al: "The immunosuppressive effect of FK506 on canine lung transplantation" J Thorac Cardiovasc Surg. 103. 1127-1135 (1992)
[Publications] Hirata.T,Ymazaki.F,Fukuse.T,Wada.H,et al: "Omentopexy for Revascularization of Free Tracheal Graft in Rat" Thoracic Cardiovascular Surgeon. 40. 178-181 (1992)
[Publications] Takahashi.Y,Ymazaki.F,Wada.H,et al: "Neovascularization Effect with He-Ne Laser in the Rat Trachea" Thorachic Cardiovascular Surgeon. 40. 288-291 (1992)
[Publications] Wada.H,Hirata.T,Inui.K,Hitomi.S et al: "Laser Doppler Velocimetry(LDV)Measurement on Lung Transplantation with Bronchial Arterial Reconstruction in Canine System" Thoracic Cardiovascular Surgeon. (1992)
[Publications] Hirai.T,Waddell.TK,Puska.JD,Patterson GA,et al: "Prolonged lung allograft survival with a short course of FK506" J Thoracic Cardiovascular Surgery. 105. 1-8 (1993)
[Publications] Inui.K,Y.Takahashi,S.Hasegawa,Wada.H,et al: "Effect of preoperative irradiation on wound healing after bronchial anastomosis in mongrel dogs" J Thoracic Cardiovascular Surgery. 106. 1059-1064 (1993)
[Publications] Hirata.T,Yokomise.H,Fukuse.T,Wada.H,et al: "Effects of Trehalose in Preservation of Canine Lung for Transplants" J Thoracic Cardiovascular Surgeon. 41. 59-63 (1993)
[Publications] Hirai.T,Waddell.TK,Puska.JD,Patterson.GA,et al: "Prolonged lung allograft survival with a short course of FK506" J Thoracic Cardiovascular Surgery. 105. 1-8 (1993)
[Publications] Hirata.T,Fukuse.T,Liu.C,Wada.H,et al: "Effects of trehalose in canine lung preservation" Surgery. 115. 102-107 (1994)
[Publications] Hirata.T,Yokomise.H,Fukuse.T,Wada.H,et al: "Successful 12-hours lung preservation with trehalose" Transplantation Proceedings. 25. 1597-1599 (1993)
[Publications] Fukuse.T,Hiratai.T,Yokomise.H,Wada.H,at al: "Combined Therapy with FK-506 and Cyclosporine-A for Canine Lung Allotransplantation;Immunosuppressive Effects and Blood Trough Levels" J Heart & Lung Transplnt. 12. 941-947 (1993)
[Publications] Liu.CL,Bando.T,Hirai.T,Hirata.T,Wada.H,et al: "ET-KYOTO SOLUTION;TREHALOSE;20-hour Canine Lung Preservation EFFECTS OF ET-KYOTO SOLUTION 20-HOUR CANINE LUNG PRESERVATION" Transplantation Proceeding. (in press). (1993)
[Publications] Bando.T,Liu.CJ,Kosaka.S,Yokomise,Wada.H,et al: "20-HOUR CANINE LUNG PRESERVATION USING NEWLY DEVELOPED SOLUTIONS CONTAINING TREHALOSE" Transplantation Proceeding. (in press). (1993)
[Publications] Yagi.K,Wada.H,Fukuse.T,Hitomi.S,et al: "INHIBITION OF REPERFUSION INJURY BY rADF/HUMAN THIOREDOXIN IN CANINE LUNG PRESERVATION" Transplantation Proceeding. (in press). (1993)
[Publications] Yokomise.H,Takahashi.Y,Inui.K,Hitomi.S,et al: "Omentoplasty for postpneumonectomy bronchopleural fistulas" European Journal of Cardiothoracic Surgery. (in press). (1993)
[Publications] Yokomise.H,Inui.K,Wada.H,Goh.T,Takahashi.M,et al: "High-Dose Irradiation Prevents Rejection of Canine Tracheal Allografts" J Thoracic Cardiovascular Surgery. (in press). (1993)
[Publications] Ohno.N,Yokomise.H,Fukuse.T,Wada.H,et al: "Preparation of In-Vivo Rat Lung Model for Ischemia-Reperfusion Injury" Thoracic Cardiovascular Surgeon. 41. 304-307 (1993)
[Publications] Hirai.T,Waddele.TK,DeCampos.KN,Slutsky.AS,et al: "A Short Course of FK506 can Induce Limited Donor-Specific Graft Acceptance" Ann Thoraic Surgery. (in press). (1993)
[Publications] Hirata.T,Yokomise.H,Fukuse.T,Wada.H,et al: "Effects of trehalose in Preservation of canine lung for transplants" Thorac Cardiovasc Surgeon. 59-63 (1993)
[Publications] Bando.T,Kosaka.S,Liu.CJ,Hirai.T,Hitomi.S,Wada.H,et al: "Effect of newly developed solutions containing trehalose on 20-hour canine lung preservation" J.Thorac Cardiovasc Surgery. (in press). (1993)
[Publications] Yokomise.H,Fukuse.T,Hirata.T,Yodoi.J,Wada.H,et al: "Effect of recombinant Human Adult T-Cell Leukemic-derived Factor(rhADF)on rat lung eperfusion injury" (in press). (1994)
[Publications] 和田洋巳、人見滋樹: "肺移植" Current insight in allergy. 7. 6-8 (1992)
[Publications] 和田洋巳、横見瀬裕保: "肺・心肺保存" 臨床胸部外科. 11. 18-21 (1992)
[Publications] 和田洋巳(集中治療吉矢生人島田康弘編): "重症疾患の病態と治療の原則-臓器移植" 集中治療の手びき. (1993)
[Publications] 人見滋樹、和田洋巳、青木稔、乾健二、横見瀬裕保: "移植治療はいま-肺移植" Ope Nursing. 6. 34-38 (1991)
[Publications] 人見滋樹、和田洋巳、青木稔、山崎文郎、横見瀬裕保、他: "肺移植の世界の現況と問題点" 日本呼吸器外科学会雑誌. 5. 606-618 (1991)
[Publications] 和田洋巳、横見瀬裕保、人見滋樹: "肺移植はどれほど呼吸器能を改善するか" 医学のあゆみ. 160. 493-495 (1992)
[Publications] 人見滋樹和田洋巳: "特集臨床医学の展望-呼吸器外科-肺移植" 日本医事新報. 3541. 7 (1992)
[Publications] 乾健二、和田洋巳: "臓器移植の現状:心移植、肺移植の現況" 免疫、Immunology Frontier. 2. 314-319 (1992)
[Publications] 和田洋巳、平井隆、長谷川誠紀、乾健二、横見瀬裕保、他: "イヌ同種肺移植におけるFK506の免疫抑制効果" Transplantation Now、今日の移植. 5. 387-391 (1992)
[Publications] 高島裕一郎、中村肇、和田洋巳、淀井淳司: "ヒトチオレドキシン(ADF)" バイオサイエンスとインダストリー. 50. 19-24 (1992)
[Publications] 乾健二、和田洋巳、人見滋樹: "肺移植" エキスパートナース臨時増刊号. 11. 80-83 (1992)
[Publications] 和田洋巳、福瀬達郎、室恒太郎、青木稔、人見滋樹、他: "臓器移植に関連した諸問題:3.肺移植問題解決の実験的検討" 日本胸部外科学会雑誌. 40. 764-765 (1992)
[Publications] 藤村重文、北村諭、清水信義、十字猛夫、和田洋巳: "座談会肺移植をめぐって" 呼吸. 12. 1096-1100 (1993)
[Publications] 大久保憲一、福瀬達郎、八木一之、横見瀬祐保、人見滋樹、和田洋巳: "臓器障害と酸素ストレス:肺移植におけるラデイカルスカベンジャーとしてのADF" Medical Immunology. 26. 517-523 (1993)
[Publications] 平井隆、Waddell.TK、松崎泰憲、人見滋樹、Patterson.GA、他: "FK506の短期大量投与による移植肺の生着効果" 今日の移植、Japanese Journal of Medicine;Transplantation Now. 6. 543-549 (1993)
[Publications] 和田洋巳、横見瀬裕保、乾健二、八木一之、青木稔、人見滋樹: "世界における肺移植の現況-外科医の立場から" 日本胸部疾患雑誌. (印刷中). (1994)

1993 Fiscal Year Annual Research Report

新しい肺保存液の開発とADF/hThioredoxineのラジカルスカベンジャー効果

Principal Investigator

和田 洋巳 京都大学, 胸部疾患研究所, 助教授 (90167205)

Research Products

[Publications] Inui.K,Schaefers.HY,Aoki.M,Wada.H,Becker.V,et al: "Effect of Methylpredonisolone and Prostacyclin on Bronchial Perfusion in Lung Transplantation" Ann Thorac Surg. 55. 464-469 (1993)

[Publications] Hasegawa.S,Yokomise.H,Wada.H,et al: "Combination use with suboptimal dosage of FK506 and Cyclosporine A in Canine lung transplantation" J Thorac Cardiovasc Surg. 104. 1340-1348 (1992)

[Publications] Hirai.T,Wada.H,Hasegawa.S,Hitomi.S,et al: "The immunosuppressive effect of FK506 on canine lung transplantation" J Thorac Cardiovasc Surg. 103. 1127-1135 (1992)

[Publications] Hirata.T,Ymazaki.F,Fukuse.T,Wada.H,et al: "Omentopexy for Revascularization of Free Tracheal Graft in Rat" Thoracic Cardiovascular Surgeon. 40. 178-181 (1992)

[Publications] Takahashi.Y,Ymazaki.F,Wada.H,et al: "Neovascularization Effect with He-Ne Laser in the Rat Trachea" Thorachic Cardiovascular Surgeon. 40. 288-291 (1992)

[Publications] Wada.H,Hirata.T,Inui.K,Hitomi.S et al: "Laser Doppler Velocimetry(LDV)Measurement on Lung Transplantation with Bronchial Arterial Reconstruction in Canine System" Thoracic Cardiovascular Surgeon. (1992)

[Publications] Hirai.T,Waddell.TK,Puska.JD,Patterson GA,et al: "Prolonged lung allograft survival with a short course of FK506" J Thoracic Cardiovascular Surgery. 105. 1-8 (1993)

[Publications] Inui.K,Y.Takahashi,S.Hasegawa,Wada.H,et al: "Effect of preoperative irradiation on wound healing after bronchial anastomosis in mongrel dogs" J Thoracic Cardiovascular Surgery. 106. 1059-1064 (1993)

[Publications] Hirata.T,Yokomise.H,Fukuse.T,Wada.H,et al: "Effects of Trehalose in Preservation of Canine Lung for Transplants" J Thoracic Cardiovascular Surgeon. 41. 59-63 (1993)

[Publications] Hirai.T,Waddell.TK,Puska.JD,Patterson.GA,et al: "Prolonged lung allograft survival with a short course of FK506" J Thoracic Cardiovascular Surgery. 105. 1-8 (1993)

[Publications] Hirata.T,Fukuse.T,Liu.C,Wada.H,et al: "Effects of trehalose in canine lung preservation" Surgery. 115. 102-107 (1994)

[Publications] Hirata.T,Yokomise.H,Fukuse.T,Wada.H,et al: "Successful 12-hours lung preservation with trehalose" Transplantation Proceedings. 25. 1597-1599 (1993)

[Publications] Fukuse.T,Hiratai.T,Yokomise.H,Wada.H,at al: "Combined Therapy with FK-506 and Cyclosporine-A for Canine Lung Allotransplantation;Immunosuppressive Effects and Blood Trough Levels" J Heart & Lung Transplnt. 12. 941-947 (1993)

[Publications] Liu.CL,Bando.T,Hirai.T,Hirata.T,Wada.H,et al: "ET-KYOTO SOLUTION;TREHALOSE;20-hour Canine Lung Preservation EFFECTS OF ET-KYOTO SOLUTION 20-HOUR CANINE LUNG PRESERVATION" Transplantation Proceeding. (in press). (1993)

[Publications] Bando.T,Liu.CJ,Kosaka.S,Yokomise,Wada.H,et al: "20-HOUR CANINE LUNG PRESERVATION USING NEWLY DEVELOPED SOLUTIONS CONTAINING TREHALOSE" Transplantation Proceeding. (in press). (1993)

[Publications] Yagi.K,Wada.H,Fukuse.T,Hitomi.S,et al: "INHIBITION OF REPERFUSION INJURY BY rADF/HUMAN THIOREDOXIN IN CANINE LUNG PRESERVATION" Transplantation Proceeding. (in press). (1993)

[Publications] Yokomise.H,Takahashi.Y,Inui.K,Hitomi.S,et al: "Omentoplasty for postpneumonectomy bronchopleural fistulas" European Journal of Cardiothoracic Surgery. (in press). (1993)

[Publications] Yokomise.H,Inui.K,Wada.H,Goh.T,Takahashi.M,et al: "High-Dose Irradiation Prevents Rejection of Canine Tracheal Allografts" J Thoracic Cardiovascular Surgery. (in press). (1993)

[Publications] Ohno.N,Yokomise.H,Fukuse.T,Wada.H,et al: "Preparation of In-Vivo Rat Lung Model for Ischemia-Reperfusion Injury" Thoracic Cardiovascular Surgeon. 41. 304-307 (1993)

[Publications] Hirai.T,Waddele.TK,DeCampos.KN,Slutsky.AS,et al: "A Short Course of FK506 can Induce Limited Donor-Specific Graft Acceptance" Ann Thoraic Surgery. (in press). (1993)

[Publications] Hirata.T,Yokomise.H,Fukuse.T,Wada.H,et al: "Effects of trehalose in Preservation of canine lung for transplants" Thorac Cardiovasc Surgeon. 59-63 (1993)

[Publications] Bando.T,Kosaka.S,Liu.CJ,Hirai.T,Hitomi.S,Wada.H,et al: "Effect of newly developed solutions containing trehalose on 20-hour canine lung preservation" J.Thorac Cardiovasc Surgery. (in press). (1993)

[Publications] Yokomise.H,Fukuse.T,Hirata.T,Yodoi.J,Wada.H,et al: "Effect of recombinant Human Adult T-Cell Leukemic-derived Factor(rhADF)on rat lung eperfusion injury" (in press). (1994)

[Publications] 和田洋巳、人見滋樹: "肺移植" Current insight in allergy. 7. 6-8 (1992)

[Publications] 和田洋巳、横見瀬裕保: "肺・心肺保存" 臨床胸部外科. 11. 18-21 (1992)

[Publications] 和田洋巳(集中治療 吉矢生人 島田康弘 編): "重症疾患の病態と治療の原則-臓器移植" 集中治療の手びき. (1993)

[Publications] 人見滋樹、和田洋巳、青木 稔、乾 健二、横見瀬裕保: "移植治療はいま-肺移植" Ope Nursing. 6. 34-38 (1991)

[Publications] 人見滋樹、和田洋巳、青木 稔、山崎文郎、横見瀬裕保、他: "肺移植の世界の現況と問題点" 日本呼吸器外科学会雑誌. 5. 606-618 (1991)

[Publications] 和田洋巳、横見瀬裕保、人見滋樹: "肺移植はどれほど呼吸器能を改善するか" 医学のあゆみ. 160. 493-495 (1992)

[Publications] 人見滋樹 和田洋巳: "特集 臨床医学の展望-呼吸器外科-肺移植" 日本医事新報. 3541. 7 (1992)

[Publications] 乾 健二、和田洋巳: "臓器移植の現状:心移植、肺移植の現況" 免疫、Immunology Frontier. 2. 314-319 (1992)

[Publications] 和田洋巳、平井隆、長谷川誠紀、乾 健二、横見瀬裕保、他: "イヌ同種肺移植におけるFK506の免疫抑制効果" Transplantation Now、今日の移植. 5. 387-391 (1992)

[Publications] 高島裕一郎、中村肇、和田洋巳、淀井淳司: "ヒトチオレドキシン(ADF)" バイオサイエンスとインダストリー. 50. 19-24 (1992)

[Publications] 乾 健二、和田洋巳、人見滋樹: "肺移植" エキスパートナース 臨時増刊号. 11. 80-83 (1992)

[Publications] 和田洋巳、福瀬達郎、室 恒太郎、青木 稔、人見滋樹、他: "臓器移植に関連した諸問題:3.肺移植問題解決の実験的検討" 日本胸部外科学会雑誌. 40. 764-765 (1992)

[Publications] 藤村重文、北村諭、清水信義、十字猛夫、和田洋巳: "座談会 肺移植をめぐって" 呼吸. 12. 1096-1100 (1993)

[Publications] 大久保憲一、福瀬達郎、八木一之、横見瀬祐保、人見滋樹、和田洋巳: "臓器障害と酸素ストレス:肺移植におけるラデイカルスカベンジャーとしてのADF" Medical Immunology. 26. 517-523 (1993)

[Publications] 平井 隆、Waddell.TK、松崎泰憲、人見滋樹、Patterson.GA、他: "FK506の短期大量投与による移植肺の生着効果" 今日の移植、Japanese Journal of Medicine;Transplantation Now. 6. 543-549 (1993)

[Publications] 和田洋巳、横見瀬裕保、乾 健二、八木一之、青木 稔、人見滋樹: "世界における肺移植の現況-外科医の立場から" 日本胸部疾患雑誌. (印刷中). (1994)

和田洋巳京都大学, 胸部疾患研究所, 助教授 (90167205)

[Publications] 和田洋巳(集中治療吉矢生人島田康弘編): "重症疾患の病態と治療の原則-臓器移植" 集中治療の手びき. (1993)

[Publications] 人見滋樹、和田洋巳、青木稔、乾健二、横見瀬裕保: "移植治療はいま-肺移植" Ope Nursing. 6. 34-38 (1991)

[Publications] 人見滋樹、和田洋巳、青木稔、山崎文郎、横見瀬裕保、他: "肺移植の世界の現況と問題点" 日本呼吸器外科学会雑誌. 5. 606-618 (1991)

[Publications] 人見滋樹和田洋巳: "特集臨床医学の展望-呼吸器外科-肺移植" 日本医事新報. 3541. 7 (1992)

[Publications] 乾健二、和田洋巳: "臓器移植の現状:心移植、肺移植の現況" 免疫、Immunology Frontier. 2. 314-319 (1992)

[Publications] 和田洋巳、平井隆、長谷川誠紀、乾健二、横見瀬裕保、他: "イヌ同種肺移植におけるFK506の免疫抑制効果" Transplantation Now、今日の移植. 5. 387-391 (1992)

[Publications] 乾健二、和田洋巳、人見滋樹: "肺移植" エキスパートナース臨時増刊号. 11. 80-83 (1992)

[Publications] 和田洋巳、福瀬達郎、室恒太郎、青木稔、人見滋樹、他: "臓器移植に関連した諸問題:3.肺移植問題解決の実験的検討" 日本胸部外科学会雑誌. 40. 764-765 (1992)

[Publications] 藤村重文、北村諭、清水信義、十字猛夫、和田洋巳: "座談会肺移植をめぐって" 呼吸. 12. 1096-1100 (1993)

[Publications] 平井隆、Waddell.TK、松崎泰憲、人見滋樹、Patterson.GA、他: "FK506の短期大量投与による移植肺の生着効果" 今日の移植、Japanese Journal of Medicine;Transplantation Now. 6. 543-549 (1993)

[Publications] 和田洋巳、横見瀬裕保、乾健二、八木一之、青木稔、人見滋樹: "世界における肺移植の現況-外科医の立場から" 日本胸部疾患雑誌. (印刷中). (1994)