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1994 Fiscal Year Final Research Report Summary

Search for novel hormones and neuropeptides

Research Project

Project/Area Number 04404084
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field 内分泌・代謝学
Research InstitutionGunma University

Principal Investigator

TATEMOTO Kazuhiko  Department of Molecular Physiology, Institute for Molecular and Cellular Regulation, Gunma University, Professor, 生体調節研究所, 教授 (60240694)

Co-Investigator(Kenkyū-buntansha) KOJIMA Itaru  Department of Cell Biology, Institute for Molecular and Cellular Regulations, Gu, 生体調節研究所, 教授 (60143492)
TAKEUCHI Toshiyuki  Gunma University, Department of Molecular Medicine, Institute for Molecular and, 生体調節研究所, 教授 (00109977)
ITOH Zen  Department of Molecular Physiology, Institute for Molecular and Cellular Regulat, 生体調節研究所, 教授 (00008294)
Project Period (FY) 1992 – 1994
Keywordsbiologically active peptide / hormone / neuropeptide / growth factor / differentiation-inducing factor / peptide isolation / peptide structure
Research Abstract

The principal aim of this proposal is to isolate novel hormones and neuropeptides using a variety of assay methods and to characterize physiological and biochemical properties of the peptides. Toward this goal, we first set up a new laboratory which is capable of carrying out peptide isolation, structural determination, chemical synthesis and various physiological and pharmacological studies, then, we carried out isolation studies and various physiological studies. In this study, we isolated and characterized that : 1)novel antimicrobial peptides, rugosins, A,B,and C from the skin of the frog, rana rugosa ; 2)a novel CCK-releasing peptide from porcine intestine ; 3)insulin-like growth factor (IGF)-II and IGF binding protein-2 from brain meningeal cells ; 4)novel motilin-related peptides from porcine intestine. We also found that : 1)meninges play a neurotrophic role in the regeneration of vasopressin nerves after hypophysectomy ; 2)biotinyl C-terminal-extended motilin can be used as a biologically active receptor probe ; 3)differentiation-inducing factor (DIF-1) raises intracellular calcium concentration and induces apoptosis in pancreatic AR42J cells ; 4)pancreastatin inhibits secretagogues-induced rise in intracellular free calcium in single rat pancreatic islet cells ; 5)GLP-1 depolarizes rat pancreatic B-cells in a Na+dependent manner ; 6)bradykinin rises the intracellular calcium concentration of pancreatic acinar AR42J cells.

  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] 立元一彦: "新しい膵外分泌調節ペプチド-バンクレアスタチン" 肝胆膵. 27(6). 977-984 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sakai,T.,Satoh,M.,Hayashi,H.,Fujikura,K.,Sano,I.,er al: "Biotinyl C-terminal-extended motilin as a biologically active recepor probe." Peptides. 15. 257-262 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 立元一彦: "消化管ホルモン-消化管ホルモンの化学と臨床応用" G.I.Research. 2(4). 418-425 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 立元一彦: "消化管ホルモン研究の新展開-化学構造と分類" 胆と膵. 15(9). 815-821 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] kubohara,Y.,Saito,Y.,Tatemoto,K.: "Differentiation-inducing factor of D.discoidem raises intracellular calcium concentration and suppresses cell growth in rat pancreatic AR42J cells." FEBS Letters. 359. 119-122 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishikawa,K.,Kabeya,K.,Shinoda,M.,Kakakai,K.,Mori,M.,Tatemoto,K.: "Meninges play a neurotrophic role in the regeneration of vasopressin nerves after hypophysectomy." Brain Research. 677. 20-28 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Suzuki,S.,Ohe,Y.,Okubo,T.,Kakegawa,T.,Tatemoto,K.: "Isolation and characterization of novel antimicrobial peptides,rugosins,A,B,and C from the skin of the frog,rana rugosa." Biochem.Biophys.Res.Commun.212. 249-254 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishikawa,K.,Ohe,Y.,Tatemoto,K.: "Synthesis and secretion of insulin-like growth factor(IGF)-II and IGF binding protein-2 by culticated brain meningeal cells." Brain Research. 697. 122-129 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kubohara,Y.,Kimura,C.,Tatemoto,K.: "Putative morphogen,DIF,of dictyostelium discoideum induces apoptosis in rat pancreatic AR42J cells." Develop.Growth Differ.359. 119-122 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 立元一彦、馬 洪涛、加藤昌克: "パンクレアスタチン:膵内外分泌を調節する局所ホルモン" 臨床消化器内科. 10(7). 929-936 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 立元一彦: "消化管ホルモン研究の新展開" Prog.Med. 15. 1793-1804 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ma,H-T.,Kato,M.,Tatemoto,K.: "Effects of pancreatatin and somatostatin on secretagogues-induced rise in intracellular free calcium in single rat pancreatic islet cells." Regulatory Peptides. (in press.).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Herzig,K-H.,Schon,I.,Tatemoto,K.,Ohe,Y.,Li,Y.,Fosch,U.R.,Owyang,C.: "Diazepam binding inhibitor is a potent cholecystokinin releasing peptide in the intestine." Proc.Natl.Acad.Sci.USA. (in press.).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kato,M.,Ma,H-T.,Tatemoto,K.: "GLP-1 depolarizes the rat pancreatic b-cells in a Na+ dependent manner." Regulatory Peptides. (in press.).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Saito,Y.,Kato,M.,Kobayashi,I.,Tatemoto,K.: "Effects of bradykinin on the intracellular calcium concentration of pancreatic acinar AR42J cells." Life Sciencces. (in press.).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ishimaru,H.,Ishikawa,K.,Ohe,Y.,Takahashi,A.,Tatemoto,K.,Maruyama,Y.: "Activation of ion handling system within the gerbil hippocampus after cerebral ischemia." Brain Research. (in press.).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Saito,Y.,Kato,M.,Kubohara,Y.,Kobayashi,I,Tatemoto,K.: "Bradykinin increases intracellular free Ca+ concentration and promotes insulin secretion in the clonal B-cell line,HIT-15 cells." Biochem.Biophys.Res.Commun.(in press.).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tatemoto, K., Mizumoto, A., Tanimoto, M., Ohe, Y.and Itoh, Z.: "Isolation and characterization of motilin-related peptides from porcine intestine." Chinese Medical Science Journal (Beijing, China). 8. 46 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sakai, T., Satoh, M., Hayashi, H., Fujikura, K., Sano, I., Koyama, H., Tartemoto, K., Itoh, Z.: "Biotinyl C-terminal-extended motilin as a biologically active recepor probe." Peptides. 15. 257-262 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kubohara, Y., Saito, Y., Tatemoto, K.: "Differentiation-inducing factor of D.discoidem raises intracellular calcium concentration and suppresses cell growth in rat pancreatic AR42J cells." FEBS Letters. 359. 119-122 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishikawa, K., Kabeya, K., Shinoda, M., Kakakai, K., Mori, M., Tatemoto, K.: "Meninges play a neurotrophic role in the regeneration of vasopressin nerves after hypophysectomy." Brain Research. 677. 20-28 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Suzuki, S., Ohe, Y., Okubo, T., Kakegawa, T., Tatemoto, K.: "Isolation and characterization of novel antimicrobial peptides, rugosins, A,B,and C from the skin of the frog, rana rugosa." Biochem.Biophys.Res.Commun.212. 249-254 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishikawa, K., Ohe, Y., Tatemoto, K.: "Synthesis and secretion of insulin-like growth factor (IGF)-II and IGF binding protein-2 by cultivated brain meningeal cells." Brain Research. 697. 122-129 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kubohara, Y., Kimura, C., Tatemoto, K.: "Putative morphogen, DIF,of dictyostelium discoideum induces apoptosis in rat pancreatic AR42J cells." Develop.Growth Differ.359. 119-122 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ma, H-T., Kato, M., and K.Tatemoto: "Effects of pancreastatin and somatostatin on secretagogues-induced rise in intracellular free calcium in single rat pancreatic islet cells." Regulatory Peptides. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Herzig, K-H., Schon, I., Tatemoto, K., Ohe, Y., Li, Y., Fosch, U.R., Owyang, C.: "Diazepam binding inhibitor is a potent cholecystokinin releasing peptide in the intestine." Proc.Natl.Acad.Sci.USA. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kato, M., Ma, H-T., and Tatemoto, K.: "GLP-1 depolarizes the rat pancreatic b-cells in a Na+ dependent manner." Regulatory Peptides. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Saito, Y., Kato, M., Kobayashi, I., and Tatemoto, K.: "Effects of bradykinin on the intracellular calcium concentration of pancreatic acinar AR42J cells." Life Sciences. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ishimaru, H., Ishikawa, K., Ohe, Y., Takahashi, A., Tatemoto, K., and Maruyama, Y.: "Activation of ion handling system within the gerbil hippocampus after cerebral ischemia." Brain Research. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Saito, Y., Kato, M., Kubohara, Y., Kobayashi, I., and Tatemoto, K.: "Bradykinin increases intracellular free Ca^+ concentration and promotes insulin secretion in the clonal B-cell line, HIT-15 cells." Biochem.Biophys.Res.Commun.(in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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