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1994 Fiscal Year Final Research Report Summary

Studies of diversification of plasmid replication regions by genetic recombination

Research Project

Project/Area Number 04454001
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 遺伝学
Research InstitutionUniversity of Electro-Communications

Principal Investigator

MIZOBUCHI Kiyoshi  University of Electro-Communications, Faculty of Electro-Communications, Professor, 電気通信学部, 教授 (00092346)

Project Period (FY) 1992 – 1994
KeywordsIncIalpha and IncFII plasmids / replication region / diversification / reciprocal recombination / RNA structures / Replication control / RNAの高次構造
Research Abstract

Living things are information systems that are highly organized by a variety of genes. In this project, we have studied how replication systems of some bacterial plasmids diverged during the course of evolution. The basic replicons of bacterial plasmids consist of two sets of genetic systems, the replication-structural system and the replication control system. Comparison of nucleotide sequences suggested that plasmids P307 (IncFI) and pMU2200 (IncZ) were generated by reciprocal recombination between ancestors of R100 (IncFII) and ColIb-P9 (IncIalpha) , or vice versa. The plasmids of each pair, P307/pMU2200 and R100/ColIb-P9, are structurally unrelated to each other. Based on this information, we constructed in vitro and analyzed P307-like chimeric replicons from ColIb-P9 and R100. When the replication-structural region of ColIb-P9 was combined with the whole replication control region of R100, the resultant replicon replicated stably as R100 did. These results revealed that the basic replicons of the plasmids diverged by exchanging their replication control systems. Thus, we propose that the replication control systems of plasmids, in some cases, evolved independently of their structural systems, although these two systems work together for maintaining the replication functions. We also studied that the reciprocal recombination was specified by the unique secondary structures of RNA involved in the control of expression of the genes encoding the replication initiator proteins.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 加藤 明彦: "Evolution of the replication regions of IncIα and IncFII plasmids by exchanging their replication control systems" DNA Research. 1. 201-212 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中山 周一: "Cloning,sequencing,and expression of bacteriophage BF23 late genes 24 and 25 encoding tail proteins" J.Bacteriol.176. 7280-7290 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 飯田 章博: "Identification and characterization of the tktB gene encoding a second transketolase in Escherichia coli" J.Bacteriol.175. 5375-5383 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 由良 隆: "Systematic sequencing of the Escherichia coli geneome: analysis of the 0-2.4 min region" Nucleic Acids Res.20. 3303-3308 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 森 淳: "A transcription terminator signal necessary for plasmid ColIb-P9 replication" Mol.Microbiol.12(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 浅野 桂: "The RNA pseudoknot structure required for translation of the replication initiation protein gene,repZ,in plasmid ColIb-P9" J.Biol.Chem.(submitted). (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akihiko Kato: "Evolution of the replication regions of IncIalpha and IncFII plasmids by exchanging their replication control systems" DNA Research. 1. 201-212 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shu-ichi Nakayama: "Cloning, sequencing, and expression of bacteriophage BF23 late genes 24 and 25 encoding tail proteins" J.Bacteriol.176. 7280-7290 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Akihiro Iida: "Identification and characterization of the tktB gene encoding a second transketolase in Escherichia coli" J.Bacteriol.175. 5375-5383 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takashi Yura: "Systematic sequencing of the Escherichia coli genome : analysis of the 0-2.4 min region" Nucleic Acids Res. 20. 3303-3308 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Atsushi Mori: "A transcriptional terminator signal necessary for plasmid ColIb-P9 replication" Mol.Microbiol.12, (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katsura Asano: "The RNA pseudoknot structure required for translation of the replication initiation protein gene, repZ,in plasmid ColIb-P9" J.Biol.Chem. (submitted). (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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