TRANSCRIPTIONAL REGULATION DURING MAMMALIAN NEUROGENESIS

1994 Fiscal Year Final Research Report Summary

• Project/Area Number: 04454002
• Research Category: Grant-in-Aid for General Scientific Research (B)
• Allocation Type: Single-year Grants
• Research Field: 遺伝学
• Research Institution: NARA INSTITUTE OF SCIENCE AND TECHNOLOGY (1994); Osaka University (1993); Nagoya University (1992)
• Principal Investigator: TAKAHASHI Naoki NARA INSTITUTE OF SCIENCE AND TECHNOLOGY,GRADUATE SCHOOL OF BIOSCIENCE,PROFESSOR, バイオサイエンス研究科, 教授 (30179501)
• Project Period (FY): 1992 – 1994
• Keywords: GENE / NERVOUS SYSTEM / DEVELOPMENT / TRANSCRIPTIONAL REGULATION / PCR / MOLECULAR BIOLOGY

Research Abstract

The homeobox is a 183-base-pare DNA sequence originally found in Drosophila segmentation and homeotic genes. InDrosophila, homeotic genes are clustered in the Antennapedia and Bithorax complexes, collectively called the homeotic gene coplex (HOM-C). In the mouse genome, about 40 homeobox genes (Hox) are clustered in four chromosomal regions (Hox A to D). The Hox genes are arranged in the same order and have the same antero-posterior patten of expression as thier structural homologue in the HOM-C,suggesting that they control mouse pattern formation in the same way that HOM-C members do in Drosophila. Homeobox gene products are believed to be transcription factors that regulate expression of target genes. A few candidate target genes have been identified in Drosophila by various approaches but the Hox gene targets are poorly understood, mostly because of limitations in the available approaches. In this research project we identify several candidate Hox gene targets, including a mouse homologue if the Drosophila tumor-suppressor gene l (2) gl, by immunopurification of DNA sequences bound to a Hox protein in native chromatin.

Research Products

• [Publications] Kuwabara,K.et.al: "mgl-1,a mouse homologue of the Drosophila tumor-suppressor gene e(2)gl,maps to chromosome 11." Genomics. 20. 337-338 (1994)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Suemori,H.et.al.: "Disruption of the HoxC-9 gene results in anterior trausformation in the vertebrae and malformation in the sternum and ribs" Mech.Dev.(in press). (1995)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] 高橋直樹: "Hox遺伝子群とその顆粒標的遺伝子の神経系における発現と機能" 細胞工学. 14. 154-159 (1995)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kondo, T., Takahashi, N., and Muramatsu, M.: "The regulation of the murine Hox-2.5 gene expression during cell differentiation" Mucl.Acids Res. 20. 5729-5735 (1992)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Goto, J., Miyabayashi, T., Wakamatsu, Y., Takahashi, N.and Muramatsu, M.: "Organization and expression of mouse Hox 3 cluster genes." Mol.Gen.Genet.239. 41-48 (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Avraham, K., Cho, , B.C., Cilbert, D., Fujii, K., Pkamoto, K., Shimazaki, T., Ito, T., Shoji, H., Wakamatsu, Y., Kondoh, H., Takahashi, N., Muramatsu, M., Hamada, N.G.and Jenkins, N.A.: "Murine chromosomal location of four class III POU transcription factors" Genomics. 18. 131-133 (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Tomotsune, D., Shoji, H., Wakamatsu, Y., Kondoh, H.and Takahashi, N.: "A mouse homologue of the Drosophila tumor-suppressor gene l (2) gl controlled by Hox-C8 in vivo.Nature" 365. 69-72 (1993)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kuwabara, K., Takahashi, Y., Tomotsune, D., Takahashi, N.and Kominami, R.: "mgl-l, a mouse homologue of the Drosophila tumor-suppressor gene l (2) gl, maps to chromosome ll.Genomics" 20. 337-338 (1994)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Suemori, H., Takahashi, N.and Noguchi, S.: "Disruption of the Hox c-9 gene results in anterior transformation in the vertebrae and malformation in the sternum and riBs.Mech.Dev." (in press.). (1995)
  • Description: 「研究成果報告書概要(欧文)」より