1993 Fiscal Year Final Research Report Summary
Elastic structure in giant myofibrillar sarcomeres
| Project/Area Number |
04454023
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
動物発生・生理学
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| Research Institution | Chiba University |
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Principal Investigator |
MARUYAMA Kohsaku Dept.Biology, Fac.Sci., Chiba Univ., Professor, 理学部, 教授 (60012267)
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| Project Period (FY) |
1992 – 1993
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| Keywords | Elastic protein / Connectin / Titin / Giant sarcomeres / Crayfish / Claw muscle |
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| Research Abstract |
In arthropod muscles, it has been known that projectin, 1200 kDa elastic protein (260 nm long), links the myosin filamentto the Z line in a sarcomere. In vertebrate skeletal muscle, connectin (titin), 3000 kDa elastic protein, does so instead.
How is the elastic structure in the giant sarcomeres of arthropod claw muscle? The sarcomere length of crayfish claw muscle is as long as 10 mum at rest. Projectin can not cover several mum distance.
In the present work it was observed that 3000 kDa connectin like protein links the Z line to the myosin filamentin crayfish closer and opener muscles. Antibodies to the 3000 kDa protein bound to the very thin filaments linking the Z line to the edge of the myosin filament after actin filaments were removed by the gelsolin treatment. Projectin was located to the surface of the myosin filament.
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Research Products
(6 results)
