1994 Fiscal Year Annual Research Report

光受容細胞における色情報伝達機構

Research Project

Project/Area Number	04454025
Research Institution	OSAKA SANGYO UNIVERSITY
Principal Investigator	吉澤透大阪産業大学, 工学部, 教授 (10028128)
Co-Investigator(Kenkyū-buntansha)	七田芳則京都大学, 理学部, 助教授 (60127090)
Keywords	アイオドプシン(Chicken Red) / Chicken Blue / Chicken Green / ロドプシン / 視物質 / トランスデューシン / 松果体 / Pinopsin
Research Abstract	1.ニワトリの網膜には、赤、緑、青、紫に特に感受性の高い4種の錐体視物質 Chicken Red,Green,Blue,Purpleがある。Chicken Blueを単離し、低温分光法を行った。他の視物質と同様、メタIとII中間体が存在した。またそれらの崩壊の時定数は、Chicken RedやGreenとほぼ同じで、ロドプシンに比べて早く崩壊することがわかった。2.赤色感受性錐体視物質であるアイオドプシン(Chicken Red)には、他の視物質と同様、メタI,II,III中間体の存在することがわかった。さらにメタI中間体は、メタII中間体へ崩壊すると共に、Chicken Redに暗再生することがわかった。3.ニワトリのロドプシンとChicken Greenとは、アミノ酸配列が互いに似ているので、特徴的に異なる3つのアミノ酸残基を選び、それらを置換したロドプシン変異体の遺伝子をサルの腎臓由来の株細胞(COS細胞)中で発現させることに成功した。現在、変異体及びその中間体の物理、化学的性質を調べている。4.ウシ網膜から精製したトランスデューシンのαサブユニット(Tα)のN末端グリシン残基には、4種類の脂肪酸がアミド結合している。これらの脂肪酸を識別する単クローン抗体を作成し、ウシの網膜切片と反応させたところ、1個の視細胞に4種のTαが存在していることを示唆する結果がえられた。5.ニワトリの松果体は概日リズムを司る時計と、これをリセットする光受容系の両者をもつ。松果体の光受容蛋白質をコードするcDNAを単離し、アミノ酸配列を決定したところ、他の視物質と約40%の相同性を示した。培養細胞系で発現させたところ、11-cis-retinalと結合し、光感受性を示した。(λmax:475nm)ので、“Pinopsin"と命名した。

Research Products

(19 results)

All Other

All Publications (19 results)

[Publications] Yoshitake Fukada et al.: "Effects of Carboxyl Methylation of Photoreceptor G Protein γ-Subunit in Visual Transduction." J.Biol.Chem.269. 5163-5170 (1994)
[Publications] Hiroko Masuda et al.: "Visual Pigments in the Pineal Complex of the Japanese Quail,Japanese Grass Lizard and Bullfrog:Immunocytochemistry and Hplc Analysis." Tissue and Cell.26. 101-113 (1994)
[Publications] Toru Yosizawa: "Molecular Basis for Color Vision." Biophysical Chemistry.50. 17-24 (1994)
[Publications] Steven W.Lin et al.: "What Makes Red Visual Pigments Red? A Resonance.Raman Microprobe Study of Retinal Chromophore Structure in Iodopsin." Biochemistry.33. 2151-2160 (1994)
[Publications] Jun Sasaki et al.: "Complete Identification of C=0 Stretching Vibrational Bands of Protonated Aspartic Acid Residues in the Difference Intrared Spectra of M and N Intermediates Versus Bacteriorhodopsin." Biochemistry.33. 3178-3184 (1994)
[Publications] Tetsuji Okada et al: "Circular Dichroism of Metaiodopsin II and its Binding to Transducin:A Comparative Study between Meta II Intermediates of Iodopsin and Rhodopsin." Biochemistry.33. 4940-4946 (1994)
[Publications] Toshiyuki Okano et al.: "Pinopsin in a Chicken Pineal Photoreceptive Molecule." Nature.485. 2575-2576 (1994)
[Publications] Junichi Hirayama et al.: "Shape of the Chromophore Binding Site in Pharaonis Phoborhodopsin from a Study Using Retinal Analogs." Photochem.Photobiol.60. 388-393 (1994)
[Publications] Yoshinori Shichida et al.: "Is Chicken Green-sensitive Cone Visual Pigment a Rhodopsin-like Pigment? A Comparative Study of the Molecular Properties Between Chicken Green and Rhodopsin." Biochemistry.33. 9040-9044 (1994)
[Publications] Yasushi Imamoto et al.: "Thermal Recovery of Idopsin from its Meta I-intermediate." FEBS Lett.354. 165-168 (1994)
[Publications] Satoshi Tamotsu et al.: "Localization of Iodopsin and Rod-opsin Immunoreactivity in the Retina and Pineal Complex of the River Lamprey,Lampetra japonica." Cell Tissue Res.278. 1-10 (1994)
[Publications] Takahiko Matsuda et al.: "Characterization of Interaction between Transducin α/βγ-subunits and Lipid Membranes." J.Biol.Chem.269. 30358-30363 (1994)
[Publications] 吉澤透ほか: "ロドプシンの光反応初期課程における発色団レチナ-ルの構造変化の測定:リングによってレチナ-ルの異性化が制限されているロドプシンアナログのピコ秒レーザー閃光分析" ビタミン. 67. 582-584 (1994)
[Publications] 吉澤透ほか: "紫外感受性視物質をめぐる最近の知見" ビタミン. 68. 182-185 (1994)
[Publications] 吉澤透ほか: "レチナ-ルタンパク質と霊長類の色覚" ビタミン. 68. 478-480 (1994)
[Publications] Daisuke Kojima et al.: "Purification and Low Temperature Spectroscopy of Gecko Visual Pigments Green and Blue." Biochemistry.(in press). (1995)
[Publications] Toru Yoshizawa et al.: "Structure and Bleaching Process of Chicken Iodopsin." Biophysical Chemistry.(in press). (1995)
[Publications] Toru Yoshizawa et al.: "CRC Handbook of Photochemistry and Photobiology.Pill-Soon Song(ed.)Vision:Photochemistry,26.1 Primary Process." CRC Press, 1477-1483. (1995)
[Publications] Hideki Kandori et al.: "Ultrafast Phenomena IX. D.Mourou et al.(ed.) Ultrafast Isomerization Dyhamics of 11-cis-locked 8-membered Rhodopsin." Springer-Verlag(in press), (1995)

1994 Fiscal Year Annual Research Report

光受容細胞における色情報伝達機構

Principal Investigator

吉澤 透 大阪産業大学, 工学部, 教授 (10028128)

Research Products

[Publications] Yoshitake Fukada et al.: "Effects of Carboxyl Methylation of Photoreceptor G Protein γ-Subunit in Visual Transduction." J.Biol.Chem.269. 5163-5170 (1994)

[Publications] Hiroko Masuda et al.: "Visual Pigments in the Pineal Complex of the Japanese Quail,Japanese Grass Lizard and Bullfrog:Immunocytochemistry and Hplc Analysis." Tissue and Cell.26. 101-113 (1994)

[Publications] Toru Yosizawa: "Molecular Basis for Color Vision." Biophysical Chemistry.50. 17-24 (1994)

[Publications] Steven W.Lin et al.: "What Makes Red Visual Pigments Red? A Resonance.Raman Microprobe Study of Retinal Chromophore Structure in Iodopsin." Biochemistry.33. 2151-2160 (1994)

[Publications] Jun Sasaki et al.: "Complete Identification of C=0 Stretching Vibrational Bands of Protonated Aspartic Acid Residues in the Difference Intrared Spectra of M and N Intermediates Versus Bacteriorhodopsin." Biochemistry.33. 3178-3184 (1994)

[Publications] Tetsuji Okada et al: "Circular Dichroism of Metaiodopsin II and its Binding to Transducin:A Comparative Study between Meta II Intermediates of Iodopsin and Rhodopsin." Biochemistry.33. 4940-4946 (1994)

[Publications] Toshiyuki Okano et al.: "Pinopsin in a Chicken Pineal Photoreceptive Molecule." Nature.485. 2575-2576 (1994)

[Publications] Junichi Hirayama et al.: "Shape of the Chromophore Binding Site in Pharaonis Phoborhodopsin from a Study Using Retinal Analogs." Photochem.Photobiol.60. 388-393 (1994)

[Publications] Yoshinori Shichida et al.: "Is Chicken Green-sensitive Cone Visual Pigment a Rhodopsin-like Pigment? A Comparative Study of the Molecular Properties Between Chicken Green and Rhodopsin." Biochemistry.33. 9040-9044 (1994)

[Publications] Yasushi Imamoto et al.: "Thermal Recovery of Idopsin from its Meta I-intermediate." FEBS Lett.354. 165-168 (1994)

[Publications] Satoshi Tamotsu et al.: "Localization of Iodopsin and Rod-opsin Immunoreactivity in the Retina and Pineal Complex of the River Lamprey,Lampetra japonica." Cell Tissue Res.278. 1-10 (1994)

[Publications] Takahiko Matsuda et al.: "Characterization of Interaction between Transducin α/βγ-subunits and Lipid Membranes." J.Biol.Chem.269. 30358-30363 (1994)

[Publications] 吉澤 透 ほか: "紫外感受性視物質をめぐる最近の知見" ビタミン. 68. 182-185 (1994)

[Publications] 吉澤 透 ほか: "レチナ-ルタンパク質と霊長類の色覚" ビタミン. 68. 478-480 (1994)

[Publications] Daisuke Kojima et al.: "Purification and Low Temperature Spectroscopy of Gecko Visual Pigments Green and Blue." Biochemistry.(in press). (1995)

[Publications] Toru Yoshizawa et al.: "Structure and Bleaching Process of Chicken Iodopsin." Biophysical Chemistry.(in press). (1995)

[Publications] Toru Yoshizawa et al.: "CRC Handbook of Photochemistry and Photobiology.Pill-Soon Song(ed.)Vision:Photochemistry,26.1 Primary Process." CRC Press, 1477-1483. (1995)

[Publications] Hideki Kandori et al.: "Ultrafast Phenomena IX. D.Mourou et al.(ed.) Ultrafast Isomerization Dyhamics of 11-cis-locked 8-membered Rhodopsin." Springer-Verlag(in press), (1995)

吉澤透大阪産業大学, 工学部, 教授 (10028128)

[Publications] 吉澤透ほか: "紫外感受性視物質をめぐる最近の知見" ビタミン. 68. 182-185 (1994)

[Publications] 吉澤透ほか: "レチナ-ルタンパク質と霊長類の色覚" ビタミン. 68. 478-480 (1994)