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1994 Fiscal Year Final Research Report Summary

Autonomous ditributed control system in the autonomic nervous system : Physiological and bio-engineering approaches

Research Project

Project/Area Number 04454035
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 計測・制御工学
Research InstitutionTokyo Medical and Dental University

Principal Investigator

KATAYAMA Yoshifumi  Tokyo Medical and Dental University Medical Research Institute Professor, 難治疾患研究所, 教授 (20014144)

Co-Investigator(Kenkyū-buntansha) MIHARA Satoshi  Kurume University School of Medicine Lecturer, 医学部, 講師 (40166103)
FURUKAWA Tetsushi  Tokyo Medical and Dental University Medical Research Institute Instructor, 難治疾患研究所, 助手 (80251552)
TATSUMI Hitoshi  Tokyo Medical and Dental University Medical Research Institute Instructor, 難治疾患研究所, 助手 (20171720)
HIRAI Kenji  Tokyo Medical and Dental University Medical Research Institute Associate Profess, 難治疾患研究所, 助教授 (70156628)
Project Period (FY) 1992 – 1994
KeywordsDistributed control system / Enteric nervous system / Autonomic nervous system / Synaptic transmission / Neurons / Neural network / Transmembrane signal transduction / Membrane pump
Research Abstract

1.The small intestine isolated from adult guinea-pigs provoked perisitaltic movement. The ability of provoking peristalsis was preserved in the intestine from which both mucosa and submucosa were mechainically removed but which contained the myenteric plexus and two smooth muscle layrs (circular and longuitudinal) . It is speculated that the peristalsis is regulated by mechanisms organized within the enteric nervous system independent from the central nervous system. Thus, the intestine is provided with an intrinsic autonomic control system with regards to the intestinal movement.
2.Actions of neuropeptides, neuropeptide Y,substance P and neurokinin A,in the enteric nervous system were analyzed using various portions of the intestine, demonstrating the evidence of regional differences in the function of the enteric neurons. ATP and L-DOPA modulated transmitter release in the enteric nervous system.
3.A large amount of Ca influx activated Ca-binding of Ca-bindeing proteins, Ca extrusion to extracellular space, Ca uptake into Ca storage sites, and Ca pump. All of these are involved in maintaining the cellular Ca homeostasis. Na-K pump alpha3 isoform was found to localize both in nerve cells and in the heart excitation conducting system, suggesting the alpha3 isoform pump function is essential to the autonomous excitation of excitable cells.
4.Growth cones of cultured neurones isolated from the diagonal band of Broca of new born rats were observed using the video-enhanced differential interference contrast microscopy. Filopodia rapidly elongated at about 1mum/s from the growth cone in response to high potassium stimulation and retracted upon the end of stimulation. The tip of folopodia moved apparently in a Brownian manner. Before making a contact with other neighboring neurons, a transmitter substance, acetylcholin, was stored and even released in and from the growth cone. These processes are supposed to play an important role in organizing neural networks.

  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Morita,K.and Katayama,Y: "Substance Pinhibits activation of calcium-dependent potassium conductances in guinea-pig myenteric neurones." Journal of Physiology(London). 447. 293-308 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 平井恵二: "神経伝達修飾物質としてのL-DOPAの作用と細胞内機序-Foa-2による細胞内Ca^<2+>動態の光学的観察" 医学のあゆみ. 162. 831-834 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mihara,S.: "Intracellular recordings from neurones of the submucous plexus." Progress in Neurobiology. 40. 529-572 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tatsumi,H.and Katayama,Y.: "Regulation of the intracellular free calcium concentration in the acutely dissociated neurones from rat nucleus basaits." Journal of Physiology(London). 464. 165-181 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tatsumi,H.,Sasaki,H.and Katayama,Y.: "Elongation of growth cone filopodia observed with video-enhaced differential interfacence contrast microscopy." Japanese Journal of Physiology. 43・suppl. S221-223 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 片山芳文: "自律神経系の機能構築と細胞生理" 病態生理. 12. 569-577 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tatsumi,H and Katayama,Y.: "Brief increases in intracellular CA^<2+> activate K^+ current and non-selevtice cation current in rat nucleus basalis neurons." Neuroscience. 58. 553-561 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kamiji,T.,Morita,K.and Katayama,Y.: "ATPregulates synaptic transmission by pre-and postsynaptic mechanisms in guinea-pig myenteric neurons." Neuroscience. 59. 165-174 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mihara,S.and Nishi,S.: "Neurokinin A mimics the slow excitatory postsynaptic current in submucous newrons of the guinea-pig caecum.21GC09:Neuroscience" 62. 1245-1255 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tatsumi,H.and Katayama,Y.: "Calcium homeostasis in the presence of fura-2 in newons dissociated from rat nucleus basaits:theoretical and experimental analysis of chelating action of fura-2." Journal of Neuroscience Methods. 53. 209-215 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Cunnigham,S.M.C.,Mihara,S.and Lees,G.M.: "Y_2-receptor-mediated selevtive inhibition of slow,inhibitory postsynaptic potential in submucous newrones of guinea-pig caecum." Britishi Journal of Pharmacology. 113. 883-888 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shen,Y-L.,Hirai,K.and Katayama,K.: "Effects of an inden-derivative,TN-871,on synaptic transmission in a sympathetic ganglion:presynaptic actions on newotransmitter release." Bull.Tokyo Mecdicaland Dental University. 42. 19-29 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Morita, K.and Katayama, Y.: "Substance P inhibits activation of calcium-dependent potassium conductances in guinea-pig myenteric neurones" Journal of Physiology (London). 447. 293-308 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirai, K.: "Neuromoculatory actions of L-DOPA and the intracellular mechanism--optical observation of intracellular Ca^<2+>-kinetics using fura-2 (in Japanese)" IGAKU no AYUMI. 162. 831-834 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mihara, S.: "Intracellular recordings from neurones of the submucous plexus" Progress in Neurobiology. 40. 529-572 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tatsummi, H.and Katayama, Y.: "Regulation of the intracellular free calcium concentration in the acutely dissociated neurones from rat nucleus basalis" Journal of Physiology (London). 464. 165-181 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tatsummi, H., Sasaki, H.and Katayama, Y.: "Elongation of growth cone filopodia observed with video-enhanced differential interference contrast microscopy" Japanese Journal of Physiology. 43, Suppl.S221-223 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Katayama, Y.: "Functional and cellular organization of the autonomic nervous system (in Japanese)" Medizina Philosophia. 12. 569-577 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tatsumi, H.and Katayama, Y.: "Brief increased in intracellular Ca^<2+> activate K^+ current and non-selective cation current in rat nucleus basalis neurons" Neuroscience. 58. 553-561 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kamiji, T., Morita, K.and Katayama, Y.: "ATP regulates synaptic transmission by pre-and postsynaptic mechanisms in guinea-pig myenteric neurons" Neuroscience. 59. 165-174 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Cunningham, S.M., Mihara, S.and Lees, G.M.: "Presynaptic inhibition by neuropeptide Y of slow inhibitory synaptic transmission in submucous neuronse of guinea-pig caecum" Experimental Physiology. 79. 261-264 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mihara, S.and Nishi, S.: "Neurokinin A mimics the slow excitatory postsynaptic current in submucous neurons of the guinea-pig caecum" Neuroscience. 62. 1245-1255 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tatsumi, H.and Katayama, Y.: "Calcium homeostasis in the presence of fura-2 in neurons dissociated from rat nucleus basalis : theoretical and experimental analysis of chelating action of fura-2" Journal of Neuroscience Methods. 53. 209-215 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Cunningham, S.M.C., Mihara, S.and Lees, G.M.: "Y_2-receptor-mediated selective inhibition of slow, inhibitory posysynaptic potential in submucous neurones of guinea-pig caecum" British Journal of Pharmacology. 113. 883-888 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shen, Y-L., Hirai, K.and Katayama, Y.: "Effects of an indene-derivative, TN-871, on synaptic transmission in a sympathetic ganglion : presynaptic action neurotransmitter release" Bull.Tokyo Medical and Dental University. 42. 19-29 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tatsumi, H.and Katayama, Y.: "Analysis of Ca^<2+> homestasis in neurons dissociated from rat nucleus basalis" Neuroscience Research. (in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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