• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1993 Fiscal Year Final Research Report Summary

Analysis of Allosteric mechanism in bacterial L-lactate dehydrogenases

Research Project

Project/Area Number 04454069
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 応用生物化学・栄養化学
Research InstitutionThe University of Tokyo

Principal Investigator

OHTA Takahisa  The Univ.of Tokyo, Faculty of Agric., Professor, 農学部, 教授 (30011844)

Project Period (FY) 1992 – 1993
KeywordsL-Lactate dehydrogenase / Allosteric / X-Ray crystallography / Protein engineering / Substrate inhibition
Research Abstract

1. X-ray crystal analysis of mutant enzyme : Mutant enzymes in which arginine-173 or histidine-188 in FBP-binding site were replaced to glutamine or tyrosine were obtained. Preliminary analysis of the enzymes showed that the histidine residue is necessary for the binding of FBP.
2. Analysis of substrate inhibition : Replace experiments showed that serine-193 and serine-318 play an important role for determining Michaelis and inhibition constants.
3. X-ray analysis of crystal which comprises both T and R forms of the LDH : Crystal analysis revealed that there are enzyme molecules in both T and R states in a single crystal. Both molecular structure were analyzed in 2.5 angstrom resolution.
4. Molecular mechanism of allosteric phenomena : Binding of FBP in T state overcomes electrostatic repulsion between subunits in P-axis. This induces rotation among four subunits and makes the active site active by interaction between subunit in Q-axis.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Koide,Shohei: "Conformational Equilibrium of an Enzymatic Catalytic Site in the Allosteric Transition." Biochemistry. 31. 5362-5368 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohta,Takahisa: "Mechanism of Allosteric Transition of Bacterial L-lactate Dehydrogenase." Faraday Discussion. 93. 153-161 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iwata,So: "Molecular Basis of Allosteric Activation of Bacterial L-Lactate Dehydrogenase." Computer Aided Innovation of New Materials. II. 1295-1298 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 岩田 想: "X線結晶構造解析によるL-乳酸脱水素酵素のアロステリック特性の解明" 日本結晶学会誌. 35. 14-20 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iwata,So: "Molecular Basis of Allosteric Activation of Bacterial L-Lactate Dehydrogenase" J.Mol.Biol.230. 21-27 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iwata,So: "Two states in crystals of bacterial L-Lactate dehydrogenase reveal the molecular mechanism for allosteric control." Nature Structural Biology. (印刷中). (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Koide, Shohei: "Conformational Equilibrium of an Enzymatic Catalytic Site in the Allosteric Transition" Biochemistry. 31. 5362-5368 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohta, Takahisa: "Mechanism of Allosteric Transition of Bacterial L-lactate Dehydrogenase" Faraday Discussion. 93. 153-161 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwata, So: "Molecular Basis of Allosteric Activation of Bacterial L-Lactate Dehydrogenase" Computer Aided Innovation of New Materials. II. 1295-1298 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwata, So: "A Study on the Allosteric Transition of L-lactate Dehydrogenase by Protein Crystallography" Nihon Kesshogaku kaisi. 35. 14-20 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwata, So: "Molecular Basis of Allosteric Activation of Bacterial L-Lactate Dehydrogenase" J.Mol.Biol.230. 21-27 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iwata, So: "Two states in crystals of bacterial L-lactate dehydrogenase reveal the molecular mechanism for allosteric control" Nature Structural Biology. (in press). (1994)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1995-03-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi