• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1993 Fiscal Year Final Research Report Summary

Studies of tissue culture on some species of Laminariales (Phaeophyta)

Research Project

Project/Area Number 04454092
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General fisheries
Research InstitutionTokyo University of Fisheries

Principal Investigator

NOTOYA Masahiro  Tokyo University of Fisheries Professor, 水産学部, 教授 (80208371)

Co-Investigator(Kenkyū-buntansha) ARUGA Yusho  Tokyo University of Fishery Professor, 水産学部, 教授 (10017022)
Project Period (FY) 1992 – 1993
KeywordsTissue culture / Laminariales / Phaeophyta
Research Abstract

In this research, to observe the influence of lght intensity and temperaure on the callus development and differentiation from blade pieces (about 0.5 x0.5 mm) exciesed from young sporophytes (about 5 mm in blade length) ofseveral species of Laminariales.
Agarum cribrosum, Alaria crassiforia, costaria costata, eckolnia cava, E.kurome, E.stolonifera, Eckloniopsis radicosa, Eisenia bicyclis, Laminaria japonica, Streptophyllopsis kuroshioensis, Undaria pinnatifida and U.undarioides were used in the experiment. Callus development and differentiation was examined under various temperatures (10, 15, 20, 25゚C) and light intensities (10, 20, 40, 80 mu mol m^<-2>s^<-1>) with a photoperiod of 14L : 10D for 32 days.
Callus cells were produced on the cut edge of explants after four days. These primodial callus cells were hemispherical in eight species of A.cribrosum, C.costata, E.cava, E.kurome, E.stolonifera, Ec. radicosa, Ei. bicyclis, L.japonica, except for four species of A.crassiforia, S.kurosh … More ioensis, U.pinnatifida, and U.undarioides in which they were club-shaped. The callus development occurred in nine species except for A.crassiforia, S.kuroshioensis, and U.undarioides. Plastids in developed callus cells were smaller than those in original blade cells. The callus development was favorable under lower light intensities and lower temperatures ; 10゚Cat all light intensities examined in A.cribrosum, 15゚C at 10-20 mu mol m^<-2>s^<-1>, in C.costata, E.kurome and E.stolenifera, 20゚C at 10-20 mu mol m^<-2>s^<-1>, in E.cova, 15゚C at 20 mu mol m^<-2>s^<-1>, in Ec. radicosa, 20゚C at 10 mu mol m^<-2>s^<-1>, in Ei. bicyclis and 15゚C at 10 mu mol m^<-2>s^<-1>, in U.pinnatifida. The differentiation of callus cells into blade occurred in seven species except for A.cribrosum and E.kurome. The earliest differentiation of callus cells into blade ocurred at higher temperatures and higher light intensities ; 20゚C at 80 mu mol m^<-2>s^<-1>, inC.costata, L.japponica and U.pinnatifida, 25゚C at 80 mu mol m^<-2>s^<-1>, in E.cava, E.stolonifera, Ec. radicosa and Ei. bicyclis. Less

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] M.Notoya: "Influence of light intensity and temperature on callus development in young sporophytes of four species of Laminariales(Phaeophyta)" Korean J.Phycol.7. 101-107 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Notoya: "Tissue culture of partheneogenetic and normal young sporophytes of Ecklonia cava Kjellman(Laminariales,Phaeophyta)" Jpn.J.Phycol.40. 53-55 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Notoya: "Formation of aposporous gametophytes from dedifferentiated young sporophyte cells of Laminaria japonica Areschoug(Laminariales,Phaeophyta)" La mar. 30. 89-91 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 能登谷正浩: "海藻の組織培養" Cell Science. 8. 110-119 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Notoya: "Tissue culture and the developmental condition of callus from young sporophytes of Eisenea bicyclis(Kjellman)Setchell(Laminariales,Phaeophyta)" Jpn.J.Phycol.40. 349-352 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Notoya: "Tissue culture of Undaria pinnatifida(Harvey)Suringar(Laminariales,Phaeophyta)" Jpn.J.Phycol.40. 393-395 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Notoya: "Influence of light intensity and temperature on callus development in young sporophytes of four species of Laminariales (Phaeophyta)" Korean J.Phycol.7(1). 101-107 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Notoya: "Tissue culture of partheneogenetic and normal young sporophytes of Ecklonia cava Kjellman (Laminariales, Pheophyta)" Jpn. J.Phycol.40. 53-55 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Notoya: "Formation of aposporous gametophytes from dedifferentiated young sporophyte cells of Laminaria japonica Areschoun (Laminariales, Phaeophyta)" La mar. 30. 89-91 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Notoya: "Tissue culture of seaweed" Cell Science. 8. 110-119 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Notoya: "Tissue culture and the developmental condition of callus from young sporophytes of Eisenia bicyclis (Kjellman) Setchell (Laminariales, Phaeophyta)" Jpn. J.Phycol. 40. 349-352 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Notoya: "Tissue culture of Undaria pinnatifida (Harvey)" Jpn. J.Phycol.40. 393-395 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Notoya: "Biotechnology of macro algae" Kaiyo Monthly. 25. 680-683 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Notoya: "Tissue culture of Laminariales species (Phaeophyta)" Kaiyo Monthly. 25. 704-710 (1993)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1995-03-27  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi