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1994 Fiscal Year Final Research Report Summary

Regulation mechanisms of transcription of sex steroidogenic enzymes during follicular growth in the chicken.

Research Project

Project/Area Number 04454106
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 畜産学(含草地学)
Research InstitutionNagoya University

Principal Investigator

SHIMADA Kiyoshi  Nagoya University, Dept.Animal Sci.Professor, 農学部, 教授 (40065579)

Co-Investigator(Kenkyū-buntansha) SAITO Noboru  Nagoya University, Dept.Animal Sci.Assist.Professor, 農学部, 助手 (40211924)
Project Period (FY) 1992 – 1994
Keywordschicken / testosterone / theca interna / theca externa / 17alphahydroxylase mRNA / aromatase mRNA / estradiol / follicular growth
Research Abstract

Concentrations of estradiol (E), testosterone (T) and progesterone (P), and levels of the 1.9-kb and 4.0-kb mRNA encoding P450_<17alpha> hydroxylase(P450_<17alpha>) and P450_<aromatase> (P450_<arom>), respectively, were measured in the isolated granulosa, theca interna and theca externa of the largest preovulatory (F1-F4) follicles of the chicken 20 h and 3 h before expected ovulation of F1. In the granulosa layr, E and T concentrations remained low throughout this period of follicular growth, whereas P concentrations were high 3 h before ovulation in F2 and F1 follicles. Estradiol levels were highest in the theca interna and externa of the amallest (F4) follicle, decreasing progressively with follicular growth. Testosterone content of theca interna and granulosa was greater in less mature F4-F2 follicles than that found in the most mature (F1) follicle, whereas theca externa contained little T.P was quantifiable in significant amounts only in granulosa tissue, peaking in F2 and F1 follicles at 3 h before ovulation, at which time plasma levels of steroids also rose significantly. P450_<17alpha> was expressed mainly in theca interna, whereas P450_<arom> mRNA levels found in theca externa refiected the pattern of E in this compartment. Expression of these two genes was very low in granulosa tissue, remaining unchanged during follicular growth. These results indicate that E and T synthesis in the chicken ovary is regulated, at least in part, at mRNA levels of P450_<17alpha> and P450_<arom> in the theca interna and theca externa layrs during follicular growth.

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] 島田 清司: "鳥類の産卵現象とその調節" 遺伝. 46. 47-52 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohkubo,et al.: "High-lcvel expression of biologically active chicken prolactin in E.coli." Comparative Biochemistry & Physiology. 105A. 123-128 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Uchiyama,M.et al.: "Pituitary and plasma arginine vasotocin levels in the lamprey,Lamperta japonica." Comparative Biochemistry & Physiology. 107A. 23-26 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sechman,A et al.: "Tissue-specific Expression of Calbindin-D_<28k> Gene During Ontogeny of the Chicken." The Journal of Expression Zoology. 269. 450-457 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kansaku,N.et al.: "Prolactin,Growth Hormone,and Luteinizing Hormone-β Subunit Gene Expression in the Cephalic and Caudal・・・" General and Comparative Endocrinology. 96. 197-205 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kato,M.et al.: "Expression of P450_<17>α-_<hydroxyrase> and P450_<aromatase> genes in isolated hranulosa,theca,interna,and theca・・・" Biology of Reproduction. 52. 405-410 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K,Shimada.,Ohkubo,T.,SaitoN,.Talbot,R.: "Avian Endocrinology" Journal of Endocrinology Ltd., 13 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] N,Saito.,Shimada,K.and Koike,T.I.: "Avian Endocrinology" Journal of Endocrinology Ltd., 8 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimada, K.: "Egg laying and its regulation (in Japanese)" The Heredity. 46. 47-52 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohkubo, T.et al.: "High-level expression of biologically active chicken prolactin in E.coli." Comparative Biochemistry & Physiology. 105A. 123-128 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Uchiyama, M.et al.: "Pituitary and plasma arginine vasotocin levels in the lamprey, Lamperta japonica." Comparative Biochemistry & Physiology. 107A. 23-26 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sechman, A.et al.: "Tissue-specific Expression of Calbindin-D_<28K> Gene During Ontogeny of the Chicken." The Journal of Expression Zoology. 269. 450-457 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kansaku, N.et al.: "Prolactin, Growth Hormone, and Luteinizing Hormone-beta Subunit Gene Expression in the Cephalic and Caudal・・・" General and Comparative Endocrinology. 96. 197-205 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kato, M.et al.: "Expression of P450_<17alpha-hydroxyrase> and P450_<aromatase> genes in isolated granulosa, theca interna, and theca・・・" Biology of Reproduction. 52. 405-410 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimada, K.et al.: Avian Endocrinology. Journal of Endocrinology Ltd., 13 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Saito, N.et al.: Avian Endocrinology. Journal of Endocrinology Ltd., 8 (1993)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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