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1993 Fiscal Year Final Research Report Summary

Inositol 1,4,5-trisphophate receptor : localization and regulation

Research Project

Project/Area Number 04454125
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General anatomy (including Histology/Embryology)
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

FUJIMOTO Toyoshi  Kyoto Univ., Fac.of Med.Associate Professor, 医学部, 助教授 (50115929)

Project Period (FY) 1992 – 1993
KeywordsInositol trisphosphate receptor / Caveola / Plasma membrane / Calcium / Calcium pump / Signal transduction / Endothelium / Smooth muscle
Research Abstract

1. Localization of inositol 1,4,5-trisphosphate receptor (IP3R) was examined by using monoclonal antibodies raised to mouse cerebellar P400 protein. One of the antibodies, 4C11, reacted with a 240-kD protein in endothelial cells, smooth muscle cells, and epidermal keratinocytes of the mouse. Immunogold labeling for the protein was observed almost exclusively in caveolae, small uncoated inpocketings of the plasma membrane.
2. Cultured bovine aortic endothelial cells were treated with a membrane-impermeable biotinylating reagent, and membrane proteins extracted with non-ionic detergent were mixed with streptavidin-agarose. The 240-kD protein was recovered by the streptavidin resin, thus proved to be exposed to the cell surface.
3. The 240-kD protein was observed along actin filaments in mouse transformed keratinocytes (PAM212) and bovine aortic endothelial cells.
By immunogold electron microscopy using rabbit polyclonal antibody to plasmalemmal Ca^<2+>-pump ATPase, the protein was localized to caveolae of various mouse cells including endothelial cells and smooth muscle cells.
5. Functions of the 240-kD protein are not known at present, but considering its similarity to IP3R, the protein may be related to Ca^<2+> influx through the plasma membrane. The results obtained in the present experiments implicate that caveolae are engaged in in and out of Ca^<2+> through the plasma membrane.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Toyoshi Fujimoto et al.: "Localization of inositol 1,4,5-trisphosphate receptor-like protein in plasmalemmal caveolae." The Journal of Cell Biology. 119. 1507-1513 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toyoshi Fujimoto: "Calcium pump of the plasma membrane is localized in caveolae" The Journal of Cell Biology. 120. 1147-1157 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 藤本 豊士: "細胞膜カベオラ" 細胞. 25. 434-438 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 藤本 豊士: "細胞膜カベオラ:細胞化学的解析" Proceedings of Clinical Electron Microscopy. 15(印刷中).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toyoshi Fujimoto et al.: "Localization of inositol 1,4,5-trisphosphate receptor-like protein in plasmalemmal caveolae." The Journal of Cell Biology. 119. 1507-1512 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toyoshi Fujimoto: "Calcium pump of the plasma membrane is localized in caveolae." The Journal of Cell Biology. 120. 1147-1157 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toyoshi Fujimoto: "Plasmalemmal caveolae." Saibo (in Japanese). 25. 434-438 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toyoshi Fujimoto: "Plasmalemmal caveola : immunocytochemical analysis." Proceedings of Clinical Electron Microscopy. 15, (in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1995-03-27  

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