1993 Fiscal Year Final Research Report Summary
Regulation of transcription factors by protein-protein interaction
| Project/Area Number |
04454161
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
General medical chemistry
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| Research Institution | The Institute of Physical & Chemical Research (RIKEN) |
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Principal Investigator |
ISHII Shunsuke RIKEN, Lab.of Molecular Genetics, Lab.Head, 分子遺伝学研究室, 主任研究員 (00124785)
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| Project Period (FY) |
1992 – 1993
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| Keywords | transcription factors / leucine zipper / inhibitor |
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| Research Abstract |
Activity of many transcription factors are regulated by protein-protein interaction. We have investigated the regulation of transcription factors by protein-protein interaction. Especially, the following transcription factors that are involved in cellular growth control were focused.
1. CRE(cAMP response element)-binding protein CRE-BP1.
We previously indentified the CRE-binding protein CRE-BP1 by cDNA cloning. CRE-BP1 binds to CRE as a homodimer or heterodimer with c-Jun. We have indentified the CRE-BP1-related protein CRE-BPa by cDNA cloning. CRE-BPa forms a homodimer or a heterodimer with c-Jun or CRE-BP1, and binds to CRE.Interestingly, the trans-activating capacity of CRE-BPa was stimulated by TPA treatment.
2. myb proto-oncogene product (Myb)
We have found that the activity of Myb is negatively regulated by the leucine zipper motif located in the middle of Myb molecule. This suggests that an inhibitor binds to Myb through this leucien zipper and blocks its activity. We have identified two proteins, 90- and 130kDa proteins, that can bind to the Myb leucien zipper. We have also found that Myb can form a dimer through the leucine zipper and a Myb dimer cannot bind to DNA.This indicates that Myb itself also can function as an inhibitor.
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