1993 Fiscal Year Final Research Report Summary
Studies of protein phophatases on immune response in normal and autoimmune disease mice.
| Project/Area Number |
04454164
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
病態医化学
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| Research Institution | HOKKAIDO UNIVERSITY |
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Principal Investigator |
KIKUCHI Kunimi HOKKAIDO UNIV., INST.IMMUNOL.SCI., PROF., 免疫科学研究所, 教授 (20006117)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUZAWA Syu-ichi HOKKAIDO UNIV., INST.IMMUNOL.SCI., RES.ASSOC., 免疫科学研究所, 助手 (80239003)
MIZUNO Yusuke HOKKAIDO UNIV., INST.IMMUNOL.SCI., ASSOC.PROF., 免疫科学研究所, 助教授 (00002121)
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| Project Period (FY) |
1992 – 1993
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| Keywords | Protein phosphatase / Autoimmune disease / Immune respose / CTLL-2 / IL-2 / PP1 / Protein dephosphorylation / Signal transduction |
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| Research Abstract |
Activities of protein phophatases PP1 and PP2A were determined in T and B Iymphocytes of autoimmune-prone MRL/MpJ-1pr/1pr mice (MRL/1pr mice) and two control strains, MRL/MpJ-+/+ mice (MRL/+/+ mice) and C3H/HeJ mice. Potential PP1 activity, which was measured after treatment of lymphocytes with Co^<2+>-trypsin, was much higher in T lymphocytes than B lymphocytes. However, no difference in the activity was observed between MRL/1pr mice and the controls. Spontaneous PP2A activity showed similar levels in T and B lymphocytes from normal mice but potential PP2A activity which was measured after treatment with 2-mercaptoethanol, was significantly higher in T lymphocytes from MRL/1pr mice than those from controls. No differences were detected in PP1 or PP2A activities in B lymphocytes.
In the IL-2-dependent murine cytotoxic T cell line CTLL-2, IL-2 induced a rapid and transient decrease in Co^<2+>-trypsin-treated activity of protein phosphatase PP1. The PP1 activity declined to a minimum level, being 70% of control value within 45 min. The decrease of PP1 activity was dependent on IL-2 concentration, and occured specfically in cytosolic fraction. Similar alteration was observed in IL-2 sensitive murine T-lymphoblasts. Neither activity of protein phophatase PP2A nor PP2C showed alteration during the IL-2 stimulation. These results suggest that PP1 plays an important role in early events of the intracellular growth signaling through the IL-2 receptor.
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[Publications] Saadat, M., Kitamura, K., Mizuno, Y., Saeki, H., Kudo, T.and Kikuchi, K.: "Gene expression of protein phosphatases in rat ascites hepatoma cell lines." Cancer Detection and Prevention. 18(1) in press. (1994)
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[Publications] Kikuchi, K., Mizuno, Y., Kitamura, K., Kakinoki, Y., Nakamura, K., Matsuzawa, S., and Saadat, M.: "Expressions and activities of protein phophatases during carcinogenesis, regeneration, and cell cycle." Advances in Protein Phosphatases. 7. 221-236 (1993)
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[Publications] Nakamura, K., Koda, T., Kakunuma, M., Matsuzawa, S., Kitamura, K., Mizuno, Y., and Kikuchi, K.: "Cell cycle dependent gene expressions and activities of protein phosphatases PP1 and PP2A in mouse NIH3T3 fibroblasts." Biochemical and Biophysical Research Communications. 185(1). 291-297 (1992)
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[Publications] Kakinoki, Y., Kitamura, K., Matsuzawa, S., Mizuno, Y., Miyazaki, T., and Kikuchi, K.: "Gene expressions and activities of protein phophatases PP1 and PP2A in rat liver regeneration after partial hepatectomy." Biochemical and Biophysical Research Communications. 185(1). 291-297 (1992)
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[Publications] Matsuzawa, S., Tamura, T., Mizuno, Y., Kobayashi, S., Okuyama, H., Tsukitani, Y., Uemura, D., and Kikuchi, K.: "Increase in potential activities of protein phosphatases PP1 and PP2A in lymphiod tissues of autommune MRL/MpJ-1pr/1pr mice." Journal of Biochemistry. 111(4). 472-477 (1992)
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「研究成果報告書概要(欧文)」より
