1993 Fiscal Year Final Research Report Summary
Human model system for angiogenesis and molecular mechanisms on function of growth factors involving in the angiogenesis
| Project/Area Number |
04454175
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Pathological medical chemistry
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| Research Institution | Kyushu University (1993)
大分医科大学 (1992) |
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Principal Investigator |
KUWANO Michihiko Kyushu University, Faculty of Medicine, Professor, 医学部, 教授 (80037431)
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| Co-Investigator(Kenkyū-buntansha) |
SATO Yasufumi Oita Medical School, Assistant, 医学部, 助手 (50178779)
KOHNO Kimitoshi Oita Medical School, Associate Professor, 医学部, 助教授 (00153479)
ONO Mayumi Kyushu University, Faculty of Medicine, Assistant Professor, 医学部, 講師 (80128347)
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| Project Period (FY) |
1992 – 1993
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| Keywords | Angiogenesis / Endothelial cells / Human omentum microvascular endothelial cells / Epidermal growth factor / Tumor necrosis factor / Cell migration / Tubulogenesis / Collagen gel |
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| Research Abstract |
We have isolated microvascular endothelial cells from human omentum and successfully cultured in vitro.
(1) This human omentum microvascular (HOME) cells were found to be most effectively migrated in response to EGF/TGF-alpha when confluent cultured HOME cells were wounded with razor. Tubulogenesis in collagen gel as well as expression of tissue-type plasminogen activator (t-PA) was also effectively enhanced when EGF/TGF-alpha was present.
(2) Treatment of HOME cells with TNF-alpha inhibited both cell migration and tubulogenesis of HOME cells. Furthermore TNF-alpha could further induce expression of IL-6, bFGF and collagenase genes in HOME cells.
(3) We could also establish a new co-culture system to determine whether co-cultured cells could induce tubulogenesis of endothelial cells in collagen gels. In this system, human keratinocytes and esophageal cancer cells could induce tubulogenesis of HOME cells possibly through paracrine control by secretory TGF-alpha. In our laboratory, we are nocoisolating genes involving in angiogenesis of HOME cells in culture system.
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[Publications] Ono, M., Okamura, K., Nakayama, Y., Tomita, M., Sato, Y., Komatsu, Y., and Kuwano, M.: "Induction of human microvascular endothelial tubular morphogenesis : Involvement of transforming growth factor-alpha" Biochem. Biophys. Res. Commun.189. 601-609 (1992)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Sato, Y., Okamura, K., Morimoto, A., Hamanaka, T., Shimada, T., Ono, M., Kohno, K., Sakata, T., and Kuwano, M.: "Indispensable of tissue-type plasminogen activator in growth facotrs-dependent tube-formation of human microvascular endothelial cells in vitro." Expt. Cell Res.264. 223-229 (1993)
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「研究成果報告書概要(欧文)」より
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[Publications] Abe, T., Okamura, K., Ono, M., Kohno, K., Mori, T., Hori, S., and Kuwano, M.: "Induction of vascular endothelial tubular morphogenesis by human glioma cells : A model systemu for tumor angiogenesis." J.Clinic. Invest.92. 54-61 (1993)
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「研究成果報告書概要(欧文)」より
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[Publications] Sato, Y., Morimoto, A., Kiue, A., Okamura, k., Hamanaka, R., Kohno, K., Kuwano, M., and Sakata, T.: "Irsogladine is a potent inhibitor of angiogenesis." FEBS Lett.322. 155-158 (1993)
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「研究成果報告書概要(欧文)」より
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[Publications] Kohno, K., Hamanaka, R., Abe, T., Nomura, Y., Morimoto, A., Izumi, H., Itoh, K., Ono, M., Nishihara, T., and Kuwano, M.: "Morphological change and destabilization of beta-actin mRNA by tumor necrosis factor in human microvascular endothelial cells." Expt. Cell. Res.208. 498-503 (1993)
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「研究成果報告書概要(欧文)」より
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[Publications] Sato, Y., Okada, F., Abe, M., Seguchi, T., Kuwano, M., Sato, S., Furuya, A., Hanai, N., and Tamaoki, T.: "The mechanisms for the activation of latent TGF-beta during co-culture of endothelial cells and smooth muscle cells : Cell specific targeting of latent TGF-beta to smooth muscle cells." J.Cell Biol.123. 1249-1254 (1993)
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