1994 Fiscal Year Annual Research Report

ヒト下垂体細胞の機能分化におけるPit-1蛋白発現の意義に関する免疫組織化学的研究

Research Project

Project/Area Number	04454183
Research Institution	Tokai University
Principal Investigator	長村義之東海大学, 医学部病理学, 教授 (10100992)
Co-Investigator(Kenkyū-buntansha)	竹腰進東海大学, 医学部病理学, 助手 (70216878) 佐藤慎吉東海大学, 医学部病理学, 講師 (80119677) 堤寛東海大学, 医学部病理学, 助教授 (80138643)
Keywords	Pit-1 / 下垂体 / 下垂体腺腫 / transcriptional factor / mRNA / 免疫組織化学
Research Abstract	Pit-1蛋白はラット下垂体細胞の核抽出物中のGHの転写を活性化する物質として同定された,下垂体特異的蛋白転写活性因子である。ラットおよび正常ヒト下垂体においてPit-1蛋白はGH・PRL・TSH産生細胞の蛋白転写を活性化し、これらの細胞の機能分化に関与するとされている.これまでに我々は抗ヒトPit-1ポリクロナール抗体を作製し,ヒト正常下垂体パラフィン切片上において,免疫組織化学的に検討し,Pit-1蛋白が多数の下垂体細胞の核に局在し,これらがGH/PRL細胞であるという知見を得た.平成6年度はこの抗体を用いて,種々の下垂体腺腫におけるPit-1蛋白の発現を検討した結果,GH産生腺腫,TSH産生腺腫において,全例Pit-1蛋白陽性であり,GH-PRL産生腺腫,PRL産生腺腫においても高率に陽性を示し,一方非機能性腺腫・ACTH産生腺腫では陽性率は低かった.二重染色・連続切片法にてPit-1陽性はGH,PRL,TSHβsubunit陽性細胞であることが確認された.以上により下垂体腺腫の機能分化/増殖にPit-1蛋白が関与することが示唆される知見を得た.さらに,in situ hybridization法による Pit-1 mRNAの検出も検討を行いつつある.また最近Pit-1と核結合蛋白,レセプターが共働するとの知見が培養細胞を用いた実験で注目されているが,現在われわれはヒトPit-1発現と,Estrogen receptor,T3 receptor,GRFreceptorとの関係を検索中である.

Research Products

(10 results)

All Other

All Publications (10 results)

[Publications] Sanno,N.,Inada,K.,Osamura,R.Y.,et al: "Expression of Pit-1 product in human pituitaries" Med Sci Res. 22. 685-687 (1994)
[Publications] Sanno,N.,Teramoto,A.,Osamura,R.Y.,et al: "Clinical and immunohistochemical studies on THS-secreting pituitary adenomas" Modern Pathol. 7. 893-899 (1994)
[Publications] Matsuno,A.,Teramoto,A.,Osamura,R.Y.,et al: "Application of biotinylated oligo-nucleotide probes to the detection of pituitary hormone mRNA using Northen blot anaylisis" Histochem J. 26. 771-777 (1994)
[Publications] Matsuno,A.,Ohsugi,Y.,Osamura,R.Y.,et al: "Ultrastructural distribution of growth hormone and prolactin mRNA in normal rat pituitary cells" Histochemistry. 102. 265-270 (1994)
[Publications] Matsuno,A.,Teramoto,A.,Osamura,R.Y.,et al: "Gigantism in sibling unrelated to multiple endocrine neoplasia" Neurosurg. 35. 952-956 (1994)
[Publications] Umemura,S.,Oda,K.,Osamura,R.Y.,et al: "Immunohistochemical characterization of “hyperplasia-adenoma sequence"in the pituitary glands of transgenic mice expressing" Tokai J Exp Clin Med. 20.
[Publications] Lloyd,R.V.,Osamura,R.Y.: "Transcription factors in normal and neoplastic pituitary tissu" MRT. In press.
[Publications] Matsuno,A.,Teramoto,A,Osamura,R.Y.et al: "Expression of plurihormonal mRNAs in somatotroph adenomas detected using non-isotopic in situ hybridization" Human Pathol. In press.
[Publications] Osamura,R.Y.: "Hormone production of the anterior pituitary cells and adenomas" Nihonijishinpo. 3627. 112-115 (1994)
[Publications] Osamura,R.Y.,Sanno,N.: "New clinico-cytofunctional classification of pituiatry adenomas" Byouritorinsyou. 12. 1338-1341 (1994)

1994 Fiscal Year Annual Research Report

ヒト下垂体細胞の機能分化におけるPit-1蛋白発現の意義に関する免疫組織化学的研究

Principal Investigator

長村 義之 東海大学, 医学部病理学, 教授 (10100992)

Research Products

[Publications] Sanno,N.,Inada,K.,Osamura,R.Y.,et al: "Expression of Pit-1 product in human pituitaries" Med Sci Res. 22. 685-687 (1994)

[Publications] Sanno,N.,Teramoto,A.,Osamura,R.Y.,et al: "Clinical and immunohistochemical studies on THS-secreting pituitary adenomas" Modern Pathol. 7. 893-899 (1994)

[Publications] Matsuno,A.,Teramoto,A.,Osamura,R.Y.,et al: "Application of biotinylated oligo-nucleotide probes to the detection of pituitary hormone mRNA using Northen blot anaylisis" Histochem J. 26. 771-777 (1994)

[Publications] Matsuno,A.,Ohsugi,Y.,Osamura,R.Y.,et al: "Ultrastructural distribution of growth hormone and prolactin mRNA in normal rat pituitary cells" Histochemistry. 102. 265-270 (1994)

[Publications] Matsuno,A.,Teramoto,A.,Osamura,R.Y.,et al: "Gigantism in sibling unrelated to multiple endocrine neoplasia" Neurosurg. 35. 952-956 (1994)

[Publications] Umemura,S.,Oda,K.,Osamura,R.Y.,et al: "Immunohistochemical characterization of “hyperplasia-adenoma sequence"in the pituitary glands of transgenic mice expressing" Tokai J Exp Clin Med. 20.

[Publications] Lloyd,R.V.,Osamura,R.Y.: "Transcription factors in normal and neoplastic pituitary tissu" MRT. In press.

[Publications] Matsuno,A.,Teramoto,A,Osamura,R.Y.et al: "Expression of plurihormonal mRNAs in somatotroph adenomas detected using non-isotopic in situ hybridization" Human Pathol. In press.

[Publications] Osamura,R.Y.: "Hormone production of the anterior pituitary cells and adenomas" Nihonijishinpo. 3627. 112-115 (1994)

[Publications] Osamura,R.Y.,Sanno,N.: "New clinico-cytofunctional classification of pituiatry adenomas" Byouritorinsyou. 12. 1338-1341 (1994)

長村義之東海大学, 医学部病理学, 教授 (10100992)