1993 Fiscal Year Final Research Report Summary
Molecular and immunological studies on mechanisms of protective immunity to schistosomiasis japonica
| Project/Area Number |
04454191
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
寄生虫学(含医用動物学）
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KOJIMA Somei Inst.of Med.Sci., The University of Tokyo, Professor, 医科学研究所, 教授 (00009622)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUMOTO Naoki Inst.of Med.Sci., The Univ.of Tokyo, Research Associate, 医科学研究所, 助手 (40239108)
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| Project Period (FY) |
1992 – 1993
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| Keywords | schistosomiasis japonica / protective immunity / monoclonal IgE / paramyosin / vaccine / squirrel monkey |
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| Research Abstract |
We have demonstrated that a mouse monoclonal IgE antibody, SJ18epsilon.1, recognizes a 97 kDa surface molecule (Sj97) of Schistosoma japonicum larvae and that the antibody induces partial but significant protection against the skin to lung-stage of S.japonicum infection. In the present study, we isolated the putative full-length cDNA of Sj97 by screening a lambdagt11 cDNA library from S.japonicum adult worms with SJ18epsilon.1. The predicted amino acid sequence of the cDNA showed highly significant homology to that of S.mansoni paramyosin, a potential vaccine candidate for schistosomiasis. The deletion mutants of S.japonicum paramyosin were expressed in E.coli and the translation product of 443 amino acid residues of paramyosin was found to be recognized by the antibody. We also tried to establish a system in which the extent of expression of cytokine mRNA could be determined by PCR.In addition, it was revealed that the squirrel monkey might provide a useful model for induction of protective immunity to schistosomiasis japonica.
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