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1994 Fiscal Year Final Research Report Summary

Study on pathogenic role of hemolysins produced by Vibrio spp.

Research Project

Project/Area Number 04454196
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 細菌学
Research InstitutionOsaka University

Principal Investigator

HONDA Takeshi  Research Institute for Microbial Diseases, Osaka University, Department of Bacterial Infections, Professor, 微生物病研究所, 教授 (60029808)

Co-Investigator(Kenkyū-buntansha) YOH Myonsun  Research Institute for Microbial Diseases, Osaka University, Department of Bacte, 微生物病研究所, 助手 (70093482)
YAMAMOTO Koichiro  Research Institute for Microbial Diseases, Osaka University, Department of Bacte, 微生物病研究所, 助教授 (30158274)
Project Period (FY) 1992 – 1994
KeywordsVibrio parahahemotyticus / Vibrio cholerae / Thermostable direct hemolysin / El tor hemolysin / Phosphorylation / Pore-formation
Research Abstract

We found that hemolysins produced by Vibrio spp.can be classified into two major groups : (1) Thermostable direct hemolysin (TDH) of Vibrio parahaemolyticus and its related hemolysin (TRH) and (2) El tor hemolysin of V.cholerae Ol. We studied on these two representative hemolysins and obtained following results.
1.TDH and TRH
(1) We discovered a new hemolysin in culture supernatant of V.parahaemolyticus and named TRH,and we also found that TRH-gene possessig bacteria always expressed urease.
(2) It was demonstrated that TDH and TRH were simultaneously produced in certain strains of V.parahaemolyticus.
TDH/TRH recognized a receptor which is present on membrane of not only sensitive but also resistant cell and then caused a phosphorylation of a 25kDa membrane protein through activation of protein kinase C,resulted in pore-formation which has 1-2nm inner diameter.
An isogenic mutant which lost TRH gene was developed and the result of the challenge with the mutant revealed that the isogenic mutant partially lose its enteropathogenicity, suggesting the possibility that TRH actually play a role in pathogenesis of V.parahaemolyticus and that factor (s) other than TRH may also involved in the pathogenicity.
2. El Tor hemolysin
(1) El Tor hemolysin is produced as a Pre-protoxin (80kDa) and passed through inner membrane with a signal peptide. In periplasmic space, the molecule becomes protoxin (79kDa) after cleavage the signal peptide. The Pro-region of the hemoysin acts as a chaperon and facilitates excretion of the protoxin through ontermembrane. The protoxin was finally modified to be an active form, mature hemolysin, by releasing Pro-region from protoxin-form. This event occurred by hemagglutinin/protease produced by Vibrio cholerae.

  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Xu,M.et al: "Demonstration and characterization of simultaneous production of a TDH and a TRH by clinical isolate of V.parahaemolvticus" Infection and Immunity. 62. 166-171 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tang,G.Q.et al.: "A mutant toxin of V.parahaemlyticus thermostable direct hemolysin which has lost hemolytic activity but retains ability to bind" Infection and Immunity. 62. 3299-3304 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Xu,M.et al.: "Construction and characterization of an isogenic mutant of V.parahaemolyticus having a deletion inthe TRH gene." Journal of Bacteriology. 176. 4754-4760 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Taniguchi,T.et al.: "Evidence that the N-terminal amino acid sequence of pilus colonization factor antigen III produced by human ETEC is similar to" Journal of Infectious Diseases. 170. 1049-1050 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ichinise,Y.et al.: "The effect on enterotoxicity of protease purified from Vibrio cholerae 01." FEMS Microbiol Letters. 115. 265-272 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyagi,K.et al.: "Successful application of enzyme-1 beled oligonucleotide probe for rapid and accurate cholera-diagnosis in a clinical diagnosis" Microbiology and Immunology. 38. 301-304 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nagayama,K.et al.: "Correlation between cell-associated mannose-sensitive hemagglutination by V.parahaemolyticus and adherence to a human colonic" FEMS Microbiology Letters. 120. 207-210 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shimaoka,M.et al.: "Development of enzyme-labeled oligonucleotide probe for mecA gene in MRSA." Journal of Clincal Microbiology. 32. 1866-1869 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Iijima,Y.et al.: "Simultaneous outbreak due to Vibrio cholerae and Shigella dysenteriae in Kenya." Lancet. 345. 69-70 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoh,M.et al.: "Evaluation of two assay kits for TDH as an indicator of TRH produced by V.parahaemolyticus." Microbiology and Immunology. 39. 157-159 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Honda,T.et al.: "A novel method to chemically immobilize antibody on nylon and its application to the rapid and diferential detection of two toxins" Clinical and Diagnostic Laboratory Immunology. 2(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 本田武司: "病気と細菌毒素" 化学同人, 159 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Xu, M.et al: "Demonstration and characterization of simultaneous production of a thermostable direct hemolysin (TDH) and a TDH-related hemolysin by a clinically isolated Vibrio parahaemolyticus strain, TH3766." Infect. Immun. 62. 166-171 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tang, G.Q.et al: "A mutant toxin of Vibrio parahaemolyticus thermostable direct hemolysin which has lost hemolytic activity but retains ability to bind to erythrocytes." Infect. Immun. 62. 3299-3304 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Xu, M.et al: "Construction and characterization of an isogenic mutant of Vibrio parahaemolyticus having a deletion in the thermostable direct hemolysin-related hemolysin gene (trh)." J.Bacteriol. 176. 4757-4760 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Taniguchi, T., et al: "Evidence that the N-terminal amino acid sequence of pilus colonization factor anti gen III produced by human enterotoxigenic Escherichia coli is similar to that of TcpA pilin of Vibrio cholerae." J.Infect. Dis. 170. 1049-1050 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ichinose, Y., et al: "The effect on enterotoxicity of protease purified from Vibrio cholerae Ol." FEMS Microbiol. Lett. 115. 265-272 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyagi, K., et al: "Successful application of enzymelabeled oligonucleotide probe for rapid and accurate cholera diagnosis in a clinical laboratory." Microbio. Immu nol. 38 (4). 301-304 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nagayama, K., et al: "Correlation between cell-asso-ciated mannose-sensitive hemagglutination by Vibrio parahaemolyticus and adherence to a human colonic cell line Caco-2." FEMS Microbiol. Lett. 120. 207-210 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shimaoka, M., et al: "Development of enzyme-labeled oligonucleotide probe for mecA gene in methicillin-resistant Staphylococcus aureus." J.Clin. Microbiol. 32. 1866-1869 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iijima, Y., et al: "Simultaneous outbreak due to Vibro. cholerae and Shigella dysenteriae in Kenya." Lancet. 345. 69-70 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoh, M., et al: "Evaluation of two assay kits for TDH as an indicator of TRH produced by V.Parahaemolytiucs." Microbiol. Immun. 39. 157-159 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Honda, T., et al: "A novel method to chemically immobilize antibody on nylon and its application to the rapid and differential detection of two toxins." Clin. Diagno. Lab.Immun. (in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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