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1993 Fiscal Year Final Research Report Summary

Analysis of rat MHC Class II genes

Research Project

Project/Area Number 04454205
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Immunology
Research Institution(Institute of Immunological Science) Hokkaido University

Principal Investigator

KAKINUMA Mitsuaki  Hokkaido University Institute of Immunological Science, Professor, 免疫科学研究所, 教授 (00001735)

Co-Investigator(Kenkyū-buntansha) KODA Toshiaki  University Institute of Immunological Science, Instructor, 免疫科学研究所, 助手 (20170186)
Project Period (FY) 1992 – 1993
KeywordsRat / MHC / RT1 / Class II genes
Research Abstract

The present investigations deal with the identification and sturctural analysis of rat major histocompatibility complex, RT1, class II genes which remain to be identified. As results, we established the complete structures of three class II genes, RT1.Ha, RT1.Hb and RT1.DOa.
1.The RT1.Ha which is homologous to human HLA-DPA has intact DNA sequence. The expression is extremey low which was undetectable conventional Northern analysis. However, by polymerase chain reaction on rat splenic total RNA as a template, correctly spliced RT1.Ha mRNA was detected.
2.The TR1.Hb which is homologous to human HLA-DPB has a 7base pair deletion in the third exon generating a premature stop codon 13 codons thereafter, thus this gene is a pseudogene.
3.The RT1.DOa is the rat homologue of HLA-DNA and H-20a whose name was adopted at the nomenclatur committee according to our investigation. Messenger RNAs of both RT1.DOa and RT1.DOb were detectable in the spleen by Northern analysis.
4.RT1 probes are useful for detection of previously undetected MHC genes of rodents with human probes. One such example was the detection of putative H-2Pa sequence in the mouse which has been belived missing from the geneome of the mouse.

  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Arimura,Y.,Tang,W.R.Koda,T.,Kakinuma,M.: "Analysis of a portion of the RTI region containing Hb,Ha and DOa class II genes" Transplant.Proc.27. 1505-1506 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Arimura,Y.,Tang,W.R.Koda,T.,Kakinuma,M.: "Structure 0f novel rat major histocmpatibility complex class II genes,RTI.Ha and rti.hb" Immunogenetics.(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Arimura,Y.,Tang,W.R.Koda,T.,Kakinuma,M.: "Cloning and analysis of a new rat major histocompatibility complex II gene,RTI DOa." Immunogenetics.(in press). (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Arimura, Y., Tang, W.R., Koda, T., and Kakinuma, M.: "Analysis of a portion of the RT1 region containing Hb, Ha and DOa class II genes." Transplant.Proc.27. 1505-1506 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Arimura, Y., Tang, W.R., Koda, T., and Kakinuma, M.: "Structure of novel rat major histocompatibility complex class II genes, RT1.Ha and RT1.Hb" Immunogenetics. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Arimura, Y., Tang, W.R., Koda, T., and Kakinuma, M.: "Cloning and analysis of a novel rat major histocompatibility complex II gene, RT1.DOa." Immunogenetics. (in press). (1995)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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