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1994 Fiscal Year Final Research Report Summary

Analysis of T cell self-antigen epitope in auto-immune model B/WF1 mice.

Research Project

Project/Area Number 04454209
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Immunology
Research InstitutionSaga Medical School

Principal Investigator

KIMOTO Masao  Professor, Department of Immunology, Saga Medical School, 医学部, 教授 (40153225)

Project Period (FY) 1992 – 1994
KeywordsT cell self-antigen epitope / B / WF1 mice / H-2 complex / Mixed haplotype / Class II molecule / Peptide motif / Auto-reactive T cell
Research Abstract

(NZB x NZW) F1 (B/WF1) mice have been studied as an animal model of SLE,a human systemic auto-immune disease. The onset and/or progression of B/WF1 auto-immune symptoms is reported to involve auto-reactive T cells and to be critically related to the heterozygosity of H-2 complex, the murine major histocompatibility complex. We analyzed auto-reactive T cell clones derived from B/WF1 mice. Specificity analysis revealed the presence of mixed haplotype Abetaz/Aalphad class II molecule-restricted auto-reactive T cell clones in B/WF1 mice. Immunoprecipitation and SDS/PAGE analysis usign anti-class II monoclonal antibody revealed that the amount of Abetaz/Aalphad class II molecules is very low compared to conventional class II molecules. Some of the auto-reactive T cell clones induced lgG anti-DNA antibody production upon transfer to young pre-autoimmune B/WF1 mice. DNA sequence analysis of mutant lines derived from transfectant APC that express mutated Abetaz/Aalphad class II molecules revealed that the amino acid at position Aalphad69 is important for the antigen recognition of these auto-reactive T cells. Analysis of bound peptides obtained by HPLC fraction of eluted peptides from affinity purified Abetaz/Aalphad class II molecules revealed a major motif as (I,F)XXXX(M,F,Y).

  • Research Products

    (41 results)

All Other

All Publications (41 results)

  • [Publications] Takashima,H.: "Characterization of T cell tolerance to hepatitis B virus(HBV)antigen in transgenic mice." Immunolgy. 75. 398-405 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Muraguchi,A.: "Expression of the CD2 molecule on human B lymphoid progenitors." Int.Immunol.4. 841-850 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyake,K.: "Requirement for VLA-4 and VLA-5 integrins in Lymphoma cells binding to and migration beneath stromal cells in culture." J.Cell Biol.119. 653-662 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ichigi,Y.: "Generation of cells with morphological and antigenic properties of microglia from cloned EBV-transformed lympoid progenitor cells derived from human fetal liver." Cell.Immunol.149. 193-207 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Gotoh,Y.: "Mixed haplotype Aβz/Aαd class II molecule in (NZBxNZW)F1 mice detectetd by T cell clones." J.Immunol.150. 4777-4787 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsuo,T.: "Cross-lniking of B cell receptor-related MB-1 molecule induces protein tyrosine phosphorylation in early B lineage cells." J.Immunol.150. 3766-3775 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kimoto,M.: "MHC heterozygosity and autoimmunity." Immuol.Today.14. 569-570 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kuwahara,K.: "Induction of tyrosine phosphorylation in human B lineage cells by crosslinking MB-1 molecule of B cell receptor-related heterodimer complex." Biochem.Biophys,Res.Commun.197. 1563-1569 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kuwahara,K.: "Identification of a 52-KD amolecule(p52) coprecipitated with the Ig receptor-related MB-1 protein that is inducibly phosphorylated by the stimulation with phorbol myristate acetate." J.Immunol.152. 2742-2752 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyake,K.: "A calcium-or manganese-dependent epitope on the integrin β1 chain recognized by a unique mAb." Int.Immunol.6. 1221-1226 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyake,K.: "Murine B cell proliferation and protection from apoptosis with an antibody against a 105 kDa molecule:Unresponsiveness of X-linked immunodeficient B cells." J.Exp.Med.180. 1217-1224 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tokushima,M.: "In vivo induction of IgG anti-DNA antibody by autoreactive mixed haplotype Aβz/Aαd MHC class II molecule-specific CD4^+T cell clones." Immunology. 83. 221-226 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Naitoh,K.: "Signal transmission through MHC class II molecules in a human B lymphoid progenitor cell line:Different signaling pathways depending on the maturational stages of B cells." Microbiol.Immunol.38. 967-976 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Koarada,S.: "Efficient isolation of mutant antigen-presenting cell lines by functional selection using T cell clones." J.Immunol. Methods. (in press.).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamashita,Y.: "A monoclonal antibody against murine CD38 homologue delivers a signal to B cells for prolongation of survival, and protection against apoptosis in vivo: Unresponsiveness of X-linked immunodeficient B cells." Immunology.(in press.).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Miyake,K.: "RP105,a novel B cell surface molecule implicated in B cell activation is a member of the leucine-rich repeat protein family." J.Immunol.(in press.).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 内藤恵子: "クラスIMHC分子への抗原ペプチドの会合" 臨床免疫. 24. 1468-1475 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 市木康久: "神経系と免疫系に共通する遺伝子機構" 臨床免疫. 25. 764-769 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 後藤祐大: "F1特異MHCクラスII分子" Annual Review 免疫1994. 202-209 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 木本雅夫: "シンポジウム 現代免疫学の動向と発展" 日本臨床麻酔学会誌. 14. 359-362 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 木本雅夫: "クローン化T細胞.山村雄一、多田富雄 編:現代免疫学." 医学書院, 8 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 後藤祐大: "Tリンパ球の抗原刺激による株化,黒木登志夫、谷口 克、押村光男 編:細胞工学ハンドブック." 羊土社, 6 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 木本雅夫: "大沢利昭,小山次郎,奥田研爾,矢田純一 編:免疫学辞典." 東京化学同人, (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 高島洋: "リンパ球のin vitro培養とクローニングT細胞クローンの樹立法IL-2を用いる方法.矢田純一,藤原道夫 編:リンパ球機能検索法." 中外医学社, 6 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 高島洋: "リンパ球のin vitro培養とクローニングリンパ球の凍結保存と回復法.矢田純一,藤原道夫 編:リンパ球機能検索法." 中外医学社, 4 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takasyima, H.: "Characterization of T cell tolerance to hepatitis B virus (HBV) antigen in transgenic mice." Immunolgy.75. 398-405 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Muraguchi, A.: "Expression of the CD2 molecule on human B lymphoid progenitors." Int.Immunol.4. 841-850 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyake, K.: "Requirement for VLA-4 and VLA-5 integrins in lymphoma cells binding to and migration beneath stromal cells in culture." J.Cell Biol.119. 653-662 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ichigi, Y.: "Generation of cells with morphological and antigenic properties of microglia from cloned EBV-transformed lympoid progenitor cells derived from human fetal liver." Cell.Immunol.149. 193-207 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Gotoh, Y.: "Mixed haplotype Abetaz/Aalphad class II molecule in (NZB x NZW) F1 mice detected by T cell clones." J.Immunol.150. 4777-4787 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuo, T.: "Cross-linking of B cell receptor-related MB-1 molecule induces protein pyrosine phosphorylation in early B lineage cells." J.Immunol.150. 3766-3775 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kimoto, M.: "(Letters), 1993. MHC heterozygosity and autoimmunity." Immuol.Today.14. 569-570

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kuwahara, K.: "Induction of tyrosine phosphorylation in human B lineage cells by crosslinking MB-1 molecule of B cell receptor-related heterodimer complex." Biochem.Biophys.Res.197. 1563-1569 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kuwahara, K.: "Identification of a 52-kDa molecule (p52) coprecipitated with the Ig receptor-related MB-1 protein that is inducibly phosphorylated by the stimulation with phorbol myristate acetate." J.Immunol.152. 2742-2752 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyake, K.: "A calcium-or manganese-dependent epitope on the integrin beta1 chain recognized by a unique mAb." Int.Immunol.6. 1221-1226 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyake, K.: "Murine B cell proliferation and protection from apoptosis with an antibody against a 105 kDa molecule : Unresponsiveness of X-linked mmunodeficient B cells." J.Exp.Med.180. 1217-1224 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tokushima, M.: "In vivo induction of IgG anti-DNA antibody autoreactive mixed haplotype Abetaz/Aalphad MHC class II molecule-specific CD4^+ T cell clones." Immunology. 83. 221-226 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Naitoh K.: "Signal transmission through MHC class II molecules in a human B lymphoid progenitor cell line : Different signaling pathways depending on the maturational stages of B cells." Microbiol.Immunol.38. 967-976 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Koarada, S.: "Efficient isolation of mutant antigen-presenting cell lines by functional selection using T cell clones." J.Immunol.Methods. (in press.).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamashita, Y.: "A monoclonal antibody against murine CD38 homologue delivers a signal to B cells for prolongation of survival, and protection against apoptosis in vivo : Unresponsiveness of X-linked immunodeficient B cells." Immunology.(in press.).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Miyake, K.: "RP105, a novel B cell surface molecule implicated in B cell activation is a member of the leucine-rich repeat protein family." J.Immunol.(in press.).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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