1994 Fiscal Year Final Research Report Summary
STUDIES ON HYBRID ARTIFICIAL LIVERS IN POINTS OF THE TECHNOLOGY FOR ORGAN RECONSTRUCTION
| Project/Area Number |
04454307
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Agro-economics
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| Research Institution | ASAHIKAWA MEDICAL COLLEGE |
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Principal Investigator |
KASAI Shinichi Asahikawa Medical College, Associate Professor, 医学部, 助教授 (40091566)
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| Co-Investigator(Kenkyū-buntansha) |
SAWA Masayuki Asahikawa Medical College, Assistant, 医学部, 助手 (70226059)
KAKISAKA Akitoshi Asahikawa Medical College, Assistant, 医学部, 助手 (60194677)
YAMAMOTO Tetsu Asahikawa Medical College, Assistant Professor, 医学部, 講師 (50125415)
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| Project Period (FY) |
1992 – 1994
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| Keywords | hybrid artificial liver / metabolic support / isolated hepatocytes / microcarrier / culture substrata |
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| Research Abstract |
Artificial liver and liver transplantation are the only way to replace the deteriorated functions of the damaged livers. However, complete replacement of the original liver functions is not yet possible for artificial livers today, and liver transplantation faces severe scarcity of donor livers.
Recently, artificial livers such as hemodialysis, hemoperfusion, and plasmapheresis have been used clinically to treat patients with liver failure, but the survival rate has not so improved. One of the reasons for this poor survival rate appears to be a lack of metabolic support capacity for the damaged livers. In this study, the development of a hybrid artificial liver was studied in points of the technology for organ reconstruction. Combination of hepatocytes and artificial materials has been attempted to reconstruct the metabolic functions of liver.
1) Natural substrata such as biomatrix of the liver and collagen, and synthetic substrata such as lactose-carrying polystirene were examined in primary culture of isolated hepatocytes, and these were useful for coating artificial materials.
2) Artificial materials such as dextran microcarriers and cellulose multiporous microcarriers were investigated of their availability for immobilizing hepatocytes in a hybrid artificial liver. Hepatocytes immobilized to these microcarriers showed better NH3 and fructose metabolism.
It was therefore suggested that these substrata and carriers were useful for the development of hybrid artificial livers.
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