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1994 Fiscal Year Final Research Report Summary

Study on implantable artificial liver

Research Project

Project/Area Number 04454332
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Digestive surgery
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

IKEDA Yoshito  Kyoto Univ., Res.Cent.Biomed.Eng., Professor, 生体医療工学研究センター, 教授 (00025909)

Co-Investigator(Kenkyū-buntansha) TABATA Yasuhiko  Kyoto Univ., Res.Cent.Biomed.Eng., Ass.Professor, 生体医療工学研究センター, 助手 (50211371)
HAYASHI Toshio  Univ.Osaka Profect., Res.Inst.Adv.Sci.Tech.Professor, 附属研究所, 教授 (90026089)
MACTANI Shunzo  Kyoto Univ., Res.Cent.Biomed.Eng., Professor, 生体医療工学研究センター, 教授 (10115933)
Project Period (FY) 1992 – 1994
KeywordsArtificial liver / Sodium alginate / poly (allyl amine) / Polyion complex / Hepatocytes / Cell encapsulation / Cell viability / Hydrogel
Research Abstract

The hybrid-typed artificial liver composed of hepatocytes and polymer matrices has been increasingly notad as a new therapeutic trial of acute liver failure. The encapsulation technology of hepatocytes by polymer hydrogels will prevent the cells from allogeneically or xenogeneically immune attack, allowing their metabolic functions to perform in the recipients. However, little has been reported on the physicochemical prooerties of polymer materials themselves although they may provide an informative suggestion for cell encapsulation. The present research was undertaken to obtain basic knowledge about the hydrogel materials applicable for artificial liver. The polyion complex membrane composed of sodium alginate and poly (allyl amine) (PAA) was selected since the cell encapsulation can be achieved under mild conditions, e.g.at 37゚C in aqueous solution. The water content and mechanical strength of the membrane or its permeability of proeteins, albumin and immunoglobulim, were similar to those of aliginate-poly (L-lysine) polyion complex membrane which has been widely used for hepatocye encapsulation. However, a big difference in the viability of rat hepatocytes was observed before and after their encapsulation process. It was found that the viability decrease was responsible for the contact of cells with calcium ions which is a necessary process for cell encapsulation. This indicates that it is important to make the contact time as short as possible for cell encapsulation.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 筏義人: "高分子と細胞との反応" 高分子. 41. 702-705 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Ito: "Implantation of Cell-Seeded Biodegradable Polymers for Tissue Reconstruction" Mat.Res.Soc.Symp.Proc.252. 359-365 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Aung: "Insulin Release from a Bioartificical Pancreas Using a Mesh Reinforced Polyvinyl Alcohol Hydrogel Tube -An In Vitro Study-" ASAIO. 39. 93-96 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Kinoshita: "Study on the efficacy of Biodegradable Poly(L-lactide)Mesh for Supporting Transplanted Particulate Cancellous Bone and Marrow: Experiment Involving Subcutaneous Implantation in Dogs" Biomaterials. 14. 729-736 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Tomihata: "Cross-Linking and Biodegradation of Native and Denatured Collagen" ACS Symposium Series 540,Polymers of Biological and Biomedical Significance,S.W.Shalaby,Et al.275-286 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Burczak: "Protein Permeation throuth Poly(vinyl Alcohol)Hydrogel Membranes" Biomaterials. 15. 231-238 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Ikeda: "Reactions Between Polymers and Cells" Kobunshi. 41 (10). 702-705 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Ito: "Implantation of Cell-Seeded Biodegradable Polymers for Tissue Reconstruction" Mat.Res.Symp.Proc.252. 359-365 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Aung: "Insulin Release from a Bioartificial Pancreas Using a Mesh Reinforced Polyvinyl Alcohol Hydrogel Tube-An In Vitro Study-" ASAIO. 39, No.2. 93-96 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y.Kinoshita: "Study on the efficacy of Biodegradable Poly (L-lactide) Mesh for Supporting Transplanted Particulate Cancellous Bone and Marrow : Experiment Involving Subcutaneous Implantation in Dogs" Biomaterials. 14, No.10. 729-736 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Tomihata: "Cross-Linking and Biodegradation of Native and Denatured Collagen" ACS Symposium Series 540, Polymers of Biological and Biomedical Significance, S.W.Shalaby, Et al.275-286 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Burczak: "Protein Permeation Through Poly (Vinyl Alcohol) hydrogel Membranes" Biomaterials. 15, No.3. 231-238 (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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