1993 Fiscal Year Final Research Report Summary
Development of a safety method to utilize a high porous fabric vascular prosthesis and neointimal formation on the prosthesis wall with this method
| Project/Area Number |
04454348
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Thoracic surgery
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| Research Institution | Yokohama City University |
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Principal Investigator |
NOISHIKI Yasuharu Yokohama City University, School of Medicine, Lecture, 医学部, 講師 (60033263)
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| Co-Investigator(Kenkyū-buntansha) |
YAMANE Yoshihisa Tottori Animal Clinical Research Center, Director, 所長
TOBE Michio Yokohama City University, School of Medicine, Lecture, 医学部, 講師 (70244431)
MATSUMOTO Akihiko Yokohama City University, School of Medicine, Professor, 医学部, 教授 (20045975)
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| Project Period (FY) |
1992 – 1993
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| Keywords | Vascular prosthesis, / Preclotting, / Autolofoue tissue transplantation. / Vascular surgery, / Endothelial cell, / Fibrinolysis phenomenon, / Subcutaneous adipose tissue, / Animal experiment. |
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| Research Abstract |
A sealing method for fabric vascular prosthesis was developed with chopped tissue fragments. A highly porous fabric vascular prosthesis was invaginated and a suspension of chopped autologous adipose connective tissue fragments was injected repeatedly till the pores were enmeshed with the fragments. Small tissue fragments oozed out from and firmly anchored within the interstices of the fabric which made the outer surface smooth. The graft was then invaginated to bring the smooth surface into the luminal side.
No blood leakage was observed in an extracorporeal in vivo shunt load test with heparin (Total 1,400 IU/kg, ACT>1,500), while a continuous bleeding was seen in a preclotted control graft (400IU/kg, ACT 657+341).
The sealed grafts were implanted in the descending thoracic aorta of 40 dogs and equal number of control grafts were used. The grafts were removed at selected time interval up to 738 days. No bleeding was observed with the sealed grafts and a thin layr of neointima was observed at 30 post operative days. Eight (20%) of the control dogs bled to death within 24 hours of implantation. The neointima in the control animals extended from the anastomotic sites slowly with complete healing observed at 217 post operative days.
These results demonstrated that the autologous adipose tissue fragments could reliably seal the highly porous fabric prosthesis while allowing rapid and complete neointima healing in dogs.
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[Publications] Y.Noishiki,Y.Yamane,Y.Tomizawa,T.Okoshi,S.Satoh,M.Mo,M.Ishii,Y.Ichikawa,K.Yamamoto,T.Kosuge: "Natural antithrombogenic surface created in vivo for an artificial heart." The 4th International Symposium on Artificial Heart and Assist Devices Springer-Verlag,Tokyo,New York, 8 (1992)
Description
「研究成果報告書概要(和文)」より
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[Publications] Y.Noishiki, Y.Yamane, Y.Tomizawa, T.Okoshi, S.Satoh, M.Mo, M.Ishii, Y.Ichikawa, K.Yamamoto, T.Kosuge: Natural antithombogenic surface created in vivo for an artificial heart. in Heart Replacement, Artificial Heart 4. eds T.Akutsu and H.Koyanagi.Springer-Verlag, Tokyo, New York, 61-68 (1992)
Description
「研究成果報告書概要(欧文)」より
