1993 Fiscal Year Final Research Report Summary
GENE EXPRESSION OF HEPATOCYTE GROWTH FACTOR (HGF) IN RENAL CELL CARCINOMA
| Project/Area Number |
04454400
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Urology
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| Research Institution | THE UNIVERSITY OF TOKYO |
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Principal Investigator |
HIGASHIHARA Eiji The University of Tokyo, FACULTY OF MEDICINE,ASSOCIATE PROFESSOR, 医学部(病), 助教授 (00092312)
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| Co-Investigator(Kenkyū-buntansha) |
HORIE Shigeo The University of Tokyo, FACULTY OF MEDICINE,INSTRUCTOR, 医学部(病), 助手 (40190243)
TAKEUCHI Takumi The University of Tokyo, FACULTY OF MEDICINE,INSTRUCTOR, 医学部(病), 助手 (90167487)
MORIYAMA Nobuo The University of Tokyo, FACULTY OF MEDICINE,ASSISTANT PROFESSOR, 医学部(病), 講師 (80143501)
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| Project Period (FY) |
1992 – 1993
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| Keywords | HEPATOCYTE GROWTH FACTOR / c-met / RENAL CELL CARCINOMA / quantitative RT-PCR / autocrine factor |
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| Research Abstract |
Hepatocyte growth factor (HGF) is found expressed not only in liver, but also in kidney. HGF has a mitogenic effect on renal tubular cells as a paracrine factor. In malignant cells, abnormal gene expression of growthh factors or derangement of intracellular signaling system are frequently noticed. We investigated the gene expression of HGF and its receptor, c-met in renal cell carcinoma. (1) Expression of HGF molecule in renal cell carcinoma was confirmed by immunohistological study and also with western blots. (2) HGF had a mitogenic effect on cultured human renal cell carcinoma cell line, ACHN.This effect was not inhibited by interferon a. (3) Gene expression of HGF and c-met was evaluated by quantitative RT-PCR.HGF mRNA increased according to the grade and stage of individual tumor. HGF mRNA in surrounding normal tissue was noticed proportionally increased with the mRNA level in the tumor. The extent of c-met gene expression was correlated with that of HGF mRNA in the tumor. (4) As a conclusion, HGF/c-met constitute a autocrine loop of growth factor in renal cell carcinoma.
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