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1994 Fiscal Year Final Research Report Summary

Study on treatment for prevention of metastasis in renal cell carcinoma

Research Project

Project/Area Number 04454406
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Urology
Research InstitutionSapporo Medical University School of Medicine

Principal Investigator

TSUKAMOTO Taiji  Sapporo Medical University School of Medicine, Associate Professor, 医学部, 助教授 (50112454)

Co-Investigator(Kenkyū-buntansha) NISHIMURA Masahiro  Sapporo Medical University School of Medicine Instructor, 医学部, 助手 (10208225)
MIYAO Noriomi  Sapporo Medical University School of Medicine Instructor, 医学部, 助手 (40200125)
ITOH Naoki  Sapporo Medical University School of Medicine Assistant Professor, 医学部, 講師 (60193504)
Project Period (FY) 1992 – 1994
Keywordsrenal cell carcinoma / metastasis prevention / tetracyline / type IV collagenolysis / adhesion molecules / cytokines
Research Abstract

In this study, we have revealed several important aspects of metastasis in renal cell carcinoma, described below. 1) Minocycline, a tetracycline derivative, inhibited experimental pulmonary metastasis of the highly metastatic subline of murine renal adenocarcinoma. In vitro study indicated that this inhibitory action of minocycline was derived from its suppressive effect on type IV collagenolysis of murine renal adenocaricnoma cells. The result suggests that the drug having such effect would be clinically applicable in the treatment for metastasis prevention in renal cell carcinoma. We are now trying to accumulate the clinical results. 2) INF-g and IL-2 were found to have suppressive effect on experimental metastasis of murine adenocarcinoma. Although both cytokines suppressed growth of the subcutaneously inoculated adenocarcinoma, IFN-g also suppressed type IV collagenolysis of the adenocarcinoma cells, suggesting that this suppressive action participated in reducing number of metastatic nodules of the lung. 3) Using an in vitro invasion assay modified by cultured human umbilical venule endothelial cells and an extracellular matrix membrane system, we found that inflammatory cytokines (IL-6, IL-1 and TNF) enhanced the in vitro invasiveness of human renal cell carcinoma cells. Augmented expression by VCAM-1 on the endothelium by these cytokines, wich resulted in enhancement of adhesion between endothelium by these cytokines, and renal cell carcinoma cells expressing VLA-4 (a ligand of VCAM-1), participated in enhancement of the invasiveness of the carcinoma cells.

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Tsukamoto,T.,et al.: "Interleukin-6 in renal cell carcinoma" Journal of Urology. 148. 1778-1782 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 塚本 泰司: "腎細胞癌の発癌および進展" 日本泌尿器科学会雑誌. 84. 21-23 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Otani,N.,Tsukamoto,T.,et al.: "In vitro invasive potential of type IV collagenolytic activity of human renal cell carcinoma cells derived from primary and metastatic lesions." Journal of Urology. 149. 1182-1185 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Otani,N.,Tsukamoto,T.,et al.: "Influence of growth factors on in vitro invasiveness and type IV collagenolysis of human cell carcinoma cells" Journal of Urology. 151. 223-226 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masumori,N.,Tsukamoto,T.,et al.: "Inhibitory effect of minocycline on in vitro invasion and experimental metastasis of mouse renal adenocarcinoma" Journal of Urology. 151. 1400-1404 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yanase,M.,Tsukamoto,T.,et al.: "Cytokines modulate in vitro invasiveness of renal cell carcinoma cells through their action on the process of cell attachment to entodhtelial cell" Journal of Urology. 153. 844-848 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsukamoto,T.,Kumamoto,Y.,et al.: "Proceedings of the 3rd International Symposium on Recent Advances in Urological Cancer Diagnosis and Treatment" Scientific Communication International Ltd., 312-314 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsukamoto, T., et al.: "Interleukin-6 in renal cell carcinoma" Jorunal of Urology. 148. 1778-1982 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Otani, N., Tsukamoto, T., et al.: "In vitro invasive potential of type IV collagenolytic activity of humanrenal cell carcinoma cells derived from primary and metastatic lesions." Jorunal of Urology. 149. 1182-1185 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Otani, N., Tsukamoto, T., et al.: "Influence of growth factors on ion vitro invasiveness and type IV collagenolysis of human cell carcinoma cells." Jorunal of Urology. 151. 223-226 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masumori, N., Tsukamoto, ., et al.: "Inhibitory effect of minocycline on in vitro invasion and experimental metastasis of mouse renal adenocarcinoma." Journal of Urology. 151. 1400-1404 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yanase, M., Tsukamoto, T., et al.: "Cytokines modulate in vitro invasiveness of renal cell carcinoma cells through their action on the process of cell attachment to endothelial cell." Journal of Urology. 153. 844-848 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsukamoto, T., Kumamoto, Y., et al.: "Prognostic factors for recurrence of renal cell carcinoma. In Proceedings of 3rd International Symposium on Recent Advance in Urological Cancer Diagnosis and Treatment. Ed.by Murphy, G., et al." Scientific Communication International Ltd.312-314 (1993)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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