1993 Fiscal Year Final Research Report Summary
Immunohistological study on immnological activity of P.gingivalis LPS -Effects on immne cells in vitro and in vivo-
| Project/Area Number |
04454474
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Conservative dentistry
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| Research Institution | Nagasaki University |
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Principal Investigator |
KATO Ihachi Nagasaki University, School of Dentistry Professor, 歯学部, 教授 (30005087)
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| Co-Investigator(Kenkyū-buntansha) |
ABE Yoshiriro Nagasaki University, School of Dentistry Research Associate, 歯学部, 助手 (70202687)
TANI Yoshiko Nagasaki University, School of Dentistry Research Associate, 歯学部, 助手 (90196436)
TANI Masahiko Nagasaki University, School of Dentistry Research Associate, 歯学部, 助手 (70188374)
ICHIMARU Eiji Nagasaki University, School of Dentistry Research Associate, 歯学部, 助手 (60223111)
HARA Yoshitaka Nagasaki University, School of Dentistry Associate Professor, 歯学部, 助教授 (60159100)
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| Project Period (FY) |
1992 – 1993
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| Keywords | P.gingivalis / Periodontal disease associated bacteria / Endotoxin / Alveolar bone resorption / TNF-alpha |
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| Research Abstract |
Two experiments were planned to clarify the immunogical activity of Porphyromonas gingivalis (P.g.) LPS.One was for the effects on alveolar bone resorption in vivo and the other was for the production of TNF-alpha by macrophages in vitro. Escherichia coli (E.coli) LPS was used as control. Each LPS was injected into murine gingiva and alveolar bone was observed histopathologically. In E.coli LPS-injected group alveolar bone resorption was found after four-time injection. On the other hand, bone resorption was delayd in P.g. LPS-injected group. Further the production of TNF-alpha by murine peritoneal macrophages stimulated with each LPS was lower in the P.g. LPS-injected group than in the E.coli LPS-injected group. These results suggested that the bone resorption activity of P.g. LPS in vivo is lower than E.coli LPS.The result of the experiment in vitro, that the production of TNF-alpha induced with P.g. LPS is lower than those with E.coli LPS, might supports this speculation.
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