1993 Fiscal Year Final Research Report Summary
Purification of precementum protein
| Project/Area Number |
04454478
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Conservative dentistry
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| Research Institution | Osaka Dental University |
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Principal Investigator |
NISHIMURA Kazuaki Osaka Dental University Dep.of Periodontology, Assistant Professor, 歯学部, 講師 (40098017)
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| Co-Investigator(Kenkyū-buntansha) |
TAKADA Kohei Osaka Dental University Dep.of Periodontology, Research Associate, 歯学部, 助手 (20103103)
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| Project Period (FY) |
1992 – 1993
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| Keywords | Precementum protein / Periodontal tissue regeneration / Chemoattractive activity |
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| Research Abstract |
Recent studies has clarified that cementum proteins are involved in periodontal tissue regeneration, especially new attachment to the exposed root surface. We have purified cell growth factor derived from a human tooth cementum (PCDGF) by gel permeation on the like molecular fractionation. PCDGF has following properties :
(1) Molecular weight (by gel permeation)
75,000 (]SY.+-。[) 5,000
(2) Isoelectric point
6.5 (]SY.+-。[) 1.0
(3) Thermal stability
PCDGF does not in activity by heat treatment at 60ﾟC for 30minutes.
(4) Physiological activity
PCDGF has chemoattractive potency to gingival fibroblasts.
(5) The chemoattractive activity was not destroyed by antibodies to PCDGF,FN,TGF-beta, GF,VN.
(6) SDS-polyacrylamide gel electrophoresis revealed that HPLC fraction eluted by 0.5M NaCl contained a protein with molecular weight 75kDa.
These data indicated that PCDGF is novel proteins which play an important role in periodontal tissue regeneration.
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