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1993 Fiscal Year Final Research Report Summary

Oncogenes and tumor markers in oral squamous cell carcinomas with special reference to clinicopathological features

Research Project

Project/Area Number 04454509
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 外科・放射線系歯学
Research InstitutionSapporo Medical University

Principal Investigator

ODAJIMA Tetsuyo  Sapporo Medical University Department of Oral Surgery Associate professor, 医学部, 講師 (00177239)

Co-Investigator(Kenkyū-buntansha) YOKOI Tosikazu  Sapporo Medical University Department of Oral Surgery Assistant Professor, 医学部, 助手 (30230634)
HIRATSUKA Hiroyosi  Sapporo Medical University Department of Oral Surgery Associate professor, 医学部, 講師 (50165180)
Project Period (FY) 1992 – 1993
Keywordsoral cancer / p53 / nm23 / mutation / E-cadherin / biological behavior / oncogene / tumor marker
Research Abstract

In view of their reported roles on biological behavior of cancer, the expressions of p53 tumor suppressor gene and nm23 tumor metastasis suppressor gene proteins were studied in 65 cases of oral squamous cell carcinoma by immunohistochemical technique. High level of expression of p53 protein was detected in 57% of these carcinomas and found to correlate with only tumor size and lymphnode metastasis, while overexpression of nm23 protein was seen in 39% with no relationship to any clinicopathological features and survival rate. However, combined protein expressions of p53 and nm23 were significantly more associated with both clinicopathological features and prognosis than p53 or nm23 a lone. Carcinomas with p53 overexpression and nm23 low expression showed more progressive states of disease and grade of histology, and poorer prognosis than those of p53 negative expression and nm23 high expression. The assessment of the combined expression of p53 with nm23 may serve to analyze biological … More behavior of oral squamous cell carcinoma. The frequencies of mutation of p53 were investigated in 36 oral swuamous cell carcinomas sing non-RI single-strand conformation polymorphism (SSCP) analysis of DNA fragments which were purified from formalin-fixed and paraffin embedded specimens and amplified by the polymerase chain reaction (PCR). p53 gene mutations were detected in 30% tumor specimens. They showed all positive overexpression for p53 in protein level. However, overexpression of p53 protein was not always related to the p53 gene mutation. Further technical improvements are needed to detect the p53 gene mutation in the routined clinical materials using non-RI PCR SSCP method.
Expression of level of E-cadherin, a cell-cell adhesion molecule, was retrospectively studied in 34 cases with oral squamous cell cacinoma by immunohistochemistry. The reduced E-cadherin expression correlated with the clinical and histological aggresiveness, resulting in cancer metastasis and invasion. It is suggested that E-cadherin is a significant marker for predicting the biological behavior of oral sqamous cell carcinoma. Less

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Hirota,S.,Odajima,T. et al.: "Significance of GST-π as a tumor marker in oral cancer." Cancer. 70. 2381-2387 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小田島哲世、小浜源郁: "舌癌の術前化学療法効果判定のためのMRらびにUS画像解析" 歯科放射線診断研究会誌. 19. 22-23 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 京極順二、小浜源郁、他: "舌扁平上皮癌の術前化学療法効果-臨床効果と組織効果-" 癌と化学療法. 19. 2381-2387 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 有馬征志、横井敏一、他: "口腔扁平上皮癌細胞株のGST-π産出について" 口腔組織培養研究会誌. 1. 31-32 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 平田章二、小田島哲世、他: "口腔癌の化学療法効果判定のための腫瘍マーカーGlutathione-S-transferase(GST)-π" 頭頸腫瘍. 19. 221-225 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Odajima T.: "Identification of apoptosis-positive cells in oral squamous cell carcinoma" Tumor Res.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Odajima T.: "Heat shock protein, HSP27, as a differentiation marker in squamous cell carcinoma" Tumor Res.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirata S., Odajima T.et al: "Significance of glutathion-S-transferase-pi as a tumor marker in patients with oral cance" Cancer. 70. 2381-23897 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Odajima t.and Kohama G.: "Effect of preoperative chemotherapy analyzed by MR and US image in tongue carcinoma" J.Jap.Dent.Radiol.Diag.19 (in Japanese). 22-23 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirata S., Odajima T., et al.: "Glutathion-S-transferase (GST) -pi as a tumor marker in assessing hte effects of chemotherapy in oral cancer" Head Neck Cancer. 19 (in Japanese). 221-225 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kyogoku J., Kohama G., et al.: "Effect of preoperative chemotherapy in squamous cell carcinoma in the tongue - Evaluation of clinical and histologic response-" Jap.J.Cancer Chemother.19 (in Japanese). 2381-2387 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Arima M., Yokoi T.et al.: "Production of GST-pi in oral squamous cell carcinoma cell lines" Tissue Culure Dent.1 (in Japanese). 31-32 (1992)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1999-03-09  

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