1993 Fiscal Year Final Research Report Summary
Development of X-ray-and Chemi-luminescence Video-Microscopy and its Application to the Analysis of Biodynamics in Micro Region
| Project/Area Number |
04454525
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Physical pharmacy
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| Research Institution | HIROSHIMA UNIVERSITY |
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Principal Investigator |
MASUJIMA Tsutomu Hiroshima University School of Medicine Professor, 医学部, 教授 (10136054)
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| Co-Investigator(Kenkyū-buntansha) |
OZAWA Kouichiro Hiroshima University School of Medicine Research Associates, 医学部, 助手 (10211822)
SUGIYAMA Masanori Hiroshima University School of Medicine Professor, 医学部, 教授 (30106801)
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| Project Period (FY) |
1992 – 1993
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| Keywords | Chemiluminescence / X-ray luminescence / Videomicroscopy / Neutrophil / Superoxide / Cell Dynamics |
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| Research Abstract |
1)Development of Chemiluminescence Video Microscopy
By assembly of a photon-counting video-camera and optical microscopy, photosensitive video-microscopy was developed which can be applied easily to the observation of dynamic aspects of biological systems in micro region.
2)Application of this Chemiluminescence Video Microscopy
The developed system was applied to the observation of phagocytosis of Neutrophils in which superoxide is generated to kill the foreign particles. This superoxide was detected as the chemiluminescence with reaction of luminor. The time course of this generation process and the amount of superoxide were successfully analyzed and showed future fruitful potential of this method.
3)X-ray Luminescent Phenomena
Irradiating the strong X-ray from synchrotron, the intensity of luminescence was measured and the intensity depends on the hydrophobisity of solvent, and was weak in aqueous solvent. This phenomena can be applied to the monitoring of hydrophobisity of probe surrounding.
4)Application of Video Analyzing System to Other Dynamic Aspects
This system was applied to the analysis of separation process of Liquid Chromatography. The band separation process in the column was directly observed by this system and the real separation process was cleared that has been only under imagination of all chromatographer.
It was shown that the developed video-analyzing system has fruitful potentials for the analysis of dynamic aspects in not only micro-region but also in macro-region.
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