1994 Fiscal Year Annual Research Report

胎盤ペプチドホルモン産生細胞の分化に関わるホメオボックス遺伝子のクローニング

Research Project

Project/Area Number	04454559
Research Institution	Nagoya University
Principal Investigator	妹尾久雄名古屋大学, 環境医学研究所, 教授 (40135380)
Co-Investigator(Kenkyū-buntansha)	宮本法博名古屋大学, 助手 (10221615) 村田善晴名古屋大学, 助教授 (80174308)
Keywords	Pax-8 / 胎盤 / サイログロブリン / redox
Research Abstract	ホメオボックス蛋白を含め多数の組織特異的転写調節因子が同定され、これらの因子が組織の分化、機能維持に重要であることが示されきた。近年、下垂体における成長ホルモン(GH)、甲状腺刺激ホルモン遺伝子(TSH)の組織特異的発現がPit-1と称される転写調節因子により行われることが明らかにされた。胎盤で合成される胎盤性ラクト-ゲン(placental lactogen)、絨毛性ゴナドトロピン(chorionic gonadotropin)などのペプチドホルモンは、それぞれ下垂体で合成されるGH,TSHと同じ祖先遺伝子から進化したと考えられるため、胎盤でのこれらホルモン産生にもPit-1が関与しているか否かを検討した。RT-PCRを用いた検討により、胎盤では、Pit-1の発現は認められず、他の転写調節因子の関与が示唆された。そこで同じPOUファミリーに属する遺伝子発現を検討したところ、Oct-1の胎盤における発現を確認した。さらにpaired domain遺伝子群に属するPax-8のも胎盤で発現していることを確認し、これら転写調節因子が胎盤での組織特異的遺伝子発現調節に関わっていることが示唆された。一方、Pax-8の活性化に関してはこれまで報告がないためその活性化機構も検討した。その結果、Pax-8は、転写因子JUN、NF-kBと同様、分子内のcystein残基の還元により活性化されることが明にされた。今後、胎盤においてPax-8により調節を受ける遺伝子群を同定したい。

Research Products
(27 results)

All Other

All Publications (27 results)

[Publications] Tsukahara S.: "Cloning of the human Pit-1 POU-box." Environmental Medicine. 36. 55-58 (1992)
[Publications] Shibata H.: "Gonadotropin induces expression of c-fos and c-jun genes in rat ovaries." Endocrinologia Japonica. 39. 455-460 (1992)
[Publications] Ohwaki M.: "Source of prolactin in human follicular fluid." Endocrinologia Japonica. 39. 601-607 (1992)
[Publications] Kambe F.: "An additional carbohydrate chain in the variant thyroxine-binding globulin-Gary (TBG^<Asn-96>)inpairs its secretion." Molecular Endocrinoogy. 6. 443-449 (1992)
[Publications] Lee J.: "The effect of thyroid hormone on fibronectin messenger ribonucleic acid levels in primary cultured rat hepatocytes." Endocrinology. 130. 2733-2738 (1992)
[Publications] Miyamoto N.: "A3′,5′-cyclic adenosine monophosphate-dependent pathway is responsible for a tapid increase in c-fos messenger ribonucleic acid by adrenocorticotropin." Endocrinology. 130. 3231-3236 (1992)
[Publications] Ohno M.: "ACTH increases expression of c-fos,c-jun and β-actin genes in the dexamethasone treated rat adrenals." Endocrinologia Japonica. 39. 377-383 (1992)
[Publications] Kambe F.: "The POU-domain protein Oct-1 is widely expressed in adult rat organs." Biochimica et Biphysica Acta. 1171. 307-310 (1993)
[Publications] 神部福司: "RT-PCR法を用いた新しいPOU遺伝子のクローニングの試み." 名古屋大学環境医学研究所年報. 44. 154-157 (1993)
[Publications] Suganuma N.: "Poly-ADP)synthesis in the anterior potuitary of the female rat throughout the extrous cycle:Study of possible relation to cell proliferation and prolactin gene expression." Journal of Endocrinological Investigation. 16. 475-480 (1993)
[Publications] Hayashi Y.: "The relative expression of mutant and nomal thyroidhormone receptor genes in patients with generalizedresistance to thyroid hormone determinedby estimation of their specific mRNA products." Journal of Clinical Endocrinology and Metabolism. 76. 64-69 (1993)
[Publications] Fujieda M.: "Effect of thyroid hormone on epidermal.growth Factor gene expression in mouse submandibular gland." Endocrinology. 132. 121-125 (1993)
[Publications] Menjo M.: "Effects of thyroid and glucocorticoid hormones on the level of messenger ribonucleic acid for iodo-thyronine thpe I 5′-deiodinase in rat primary hepatocyte cultures grown as spheroids." Endocrinology. 133. 2984-2990 (1993)
[Publications] Miura Y.: "Sequence of a variant thyroxine-binding globulin(TBG)in a family with partial TBG deficiency in Japanese(TBG-PDJ)." Endocrine Journal. 40. 127-132 (1993)
[Publications] 妹尾久雄: "成長ホルモン遺伝子群の発現調節-組織特異的発現調節を中心に-" 医学のあゆみ. 165. 247-251 (1993)
[Publications] Hayashi Y.: "Human thyroxine-binding globulin gene:Complete sequence and transcriptional regulation." Molecular Endocrinology. 7. 1049-1060 (1993)
[Publications] 神部福司: "トランスフェクションを用いたTBG異常症発症機序の研究." Modern Physician. 13. 939-944 (1993)
[Publications] 神部福司: "Paired domain遺伝子に属するPax-8mRNAのラット胎盤における検出" 名古屋大学環境医学研究所年報. 45. 159-160 (1994)
[Publications] 神部福司: "ヒト血中甲状腺ホルモン輸送蛋白TBGのcDNAおよびゲノム遺伝子のクローニング." 日本臨床. 52. 875-879 (1994)
[Publications] Tsukahara S.: "Increase in Pit-1 mRNA is not required for the estrogen-induced expression of prolactin gene and lactotroph proliferation." Endocrine Journal. 41. 579-584 (1994)
[Publications] Tsukamoto N.: "Octreotide treatment results in the inhibition of GH gene expression in the adenoma of the patients with acrogmegaly." Endocrine Journal. 41. 437-444 (1994)
[Publications] Hayamizu S.: "Glucocorticoids potentiate the action of atrial natriuretic polypeptide in adrenalectomized rats." Endocrinology.135. 2459-2464 (1994)
[Publications] Miura Y.: "A truncated TBG due to a frameshift mutation is retained within the rough endoplasmic reticulum:A possible mechanism of complete TBG deficiency in Japanese." Journal of Clinical Endocrinology and Metabolism. 78. 283-287 (1994)
[Publications] Miura Y.: "Impaired intracellular transport contributes to partial thyroxine-binding globulin deficiency in a Japanese Family." Journal of Clinical Endocrinology and Metabolism. 79. 740-744 (1994)
[Publications] Matsuyama Morita T.: "Adrenocorticotropic hormone(ACTH)increases the expression of its own receptor gene." Endocrine Journal. (in press). (1995)
[Publications] 宮崎高志: "妊娠経過中のヒト胎盤遺伝子発現の変化." 名古屋大学環境医学研究所年報. 45(印刷中). (1995)
[Publications] 神部福司: "転写調節因子TTF-1,Pax-8はサイログロブリン遺伝子プロモーターに結合する." 名古屋大学環境医学研究所年報. 45(印刷中). (1995)

1994 Fiscal Year Annual Research Report

胎盤ペプチドホルモン産生細胞の分化に関わるホメオボックス遺伝子のクローニング

Principal Investigator

妹尾 久雄 名古屋大学, 環境医学研究所, 教授 (40135380)

Research Products

[Publications] Tsukahara S.: "Cloning of the human Pit-1 POU-box." Environmental Medicine. 36. 55-58 (1992)

[Publications] Shibata H.: "Gonadotropin induces expression of c-fos and c-jun genes in rat ovaries." Endocrinologia Japonica. 39. 455-460 (1992)

[Publications] Ohwaki M.: "Source of prolactin in human follicular fluid." Endocrinologia Japonica. 39. 601-607 (1992)

[Publications] Kambe F.: "An additional carbohydrate chain in the variant thyroxine-binding globulin-Gary (TBG^<Asn-96>)inpairs its secretion." Molecular Endocrinoogy. 6. 443-449 (1992)

[Publications] Lee J.: "The effect of thyroid hormone on fibronectin messenger ribonucleic acid levels in primary cultured rat hepatocytes." Endocrinology. 130. 2733-2738 (1992)

[Publications] Miyamoto N.: "A3′,5′-cyclic adenosine monophosphate-dependent pathway is responsible for a tapid increase in c-fos messenger ribonucleic acid by adrenocorticotropin." Endocrinology. 130. 3231-3236 (1992)

[Publications] Ohno M.: "ACTH increases expression of c-fos,c-jun and β-actin genes in the dexamethasone treated rat adrenals." Endocrinologia Japonica. 39. 377-383 (1992)

[Publications] Kambe F.: "The POU-domain protein Oct-1 is widely expressed in adult rat organs." Biochimica et Biphysica Acta. 1171. 307-310 (1993)

[Publications] 神部福司: "RT-PCR法を用いた新しいPOU遺伝子のクローニングの試み." 名古屋大学環境医学研究所年報. 44. 154-157 (1993)

[Publications] Suganuma N.: "Poly-ADP)synthesis in the anterior potuitary of the female rat throughout the extrous cycle:Study of possible relation to cell proliferation and prolactin gene expression." Journal of Endocrinological Investigation. 16. 475-480 (1993)

[Publications] Hayashi Y.: "The relative expression of mutant and nomal thyroidhormone receptor genes in patients with generalizedresistance to thyroid hormone determinedby estimation of their specific mRNA products." Journal of Clinical Endocrinology and Metabolism. 76. 64-69 (1993)

[Publications] Fujieda M.: "Effect of thyroid hormone on epidermal.growth Factor gene expression in mouse submandibular gland." Endocrinology. 132. 121-125 (1993)

[Publications] Menjo M.: "Effects of thyroid and glucocorticoid hormones on the level of messenger ribonucleic acid for iodo-thyronine thpe I 5′-deiodinase in rat primary hepatocyte cultures grown as spheroids." Endocrinology. 133. 2984-2990 (1993)

[Publications] Miura Y.: "Sequence of a variant thyroxine-binding globulin(TBG)in a family with partial TBG deficiency in Japanese(TBG-PDJ)." Endocrine Journal. 40. 127-132 (1993)

[Publications] 妹尾久雄: "成長ホルモン遺伝子群の発現調節-組織特異的発現調節を中心に-" 医学のあゆみ. 165. 247-251 (1993)

[Publications] Hayashi Y.: "Human thyroxine-binding globulin gene:Complete sequence and transcriptional regulation." Molecular Endocrinology. 7. 1049-1060 (1993)

[Publications] 神部福司: "トランスフェクションを用いたTBG異常症発症機序の研究." Modern Physician. 13. 939-944 (1993)

[Publications] 神部福司: "Paired domain遺伝子に属するPax-8mRNAのラット胎盤における検出" 名古屋大学環境医学研究所年報. 45. 159-160 (1994)

[Publications] 神部福司: "ヒト血中甲状腺ホルモン輸送蛋白TBGのcDNAおよびゲノム遺伝子のクローニング." 日本臨床. 52. 875-879 (1994)

[Publications] Tsukahara S.: "Increase in Pit-1 mRNA is not required for the estrogen-induced expression of prolactin gene and lactotroph proliferation." Endocrine Journal. 41. 579-584 (1994)

[Publications] Tsukamoto N.: "Octreotide treatment results in the inhibition of GH gene expression in the adenoma of the patients with acrogmegaly." Endocrine Journal. 41. 437-444 (1994)

[Publications] Hayamizu S.: "Glucocorticoids potentiate the action of atrial natriuretic polypeptide in adrenalectomized rats." Endocrinology.135. 2459-2464 (1994)

[Publications] Miura Y.: "A truncated TBG due to a frameshift mutation is retained within the rough endoplasmic reticulum:A possible mechanism of complete TBG deficiency in Japanese." Journal of Clinical Endocrinology and Metabolism. 78. 283-287 (1994)

[Publications] Miura Y.: "Impaired intracellular transport contributes to partial thyroxine-binding globulin deficiency in a Japanese Family." Journal of Clinical Endocrinology and Metabolism. 79. 740-744 (1994)

[Publications] Matsuyama Morita T.: "Adrenocorticotropic hormone(ACTH)increases the expression of its own receptor gene." Endocrine Journal. (in press). (1995)

[Publications] 宮崎高志: "妊娠経過中のヒト胎盤遺伝子発現の変化." 名古屋大学環境医学研究所年報. 45(印刷中). (1995)

[Publications] 神部福司: "転写調節因子TTF-1,Pax-8はサイログロブリン遺伝子プロモーターに結合する." 名古屋大学環境医学研究所年報. 45(印刷中). (1995)

妹尾久雄名古屋大学, 環境医学研究所, 教授 (40135380)