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1993 Fiscal Year Final Research Report Summary

Molecular biological study of viruses in type 1 disabetes mellitus

Research Project

Project/Area Number 04454562
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 内分泌・代謝学
Research InstitutionOsaka University

Principal Investigator

HANAFUSA Toshiaki  Osaka Univ Med Sch, 2nd Dept Med, Assistant Prof, 医学部, 助手 (60164886)

Co-Investigator(Kenkyū-buntansha) NAMBA Mitsuyoshi  Osaka Univ Med Sch, 2nd Dept Med, Assistant Prof, 医学部, 助手 (00183533)
Project Period (FY) 1992 – 1993
Keywordstype 1 diabetes mellitus / genetics / autoimmunity / virus / NOD mouse / insulitis / retrovirus / cloning
Research Abstract

Type 1(insulin-dependent) diabetes mellitus is thought to develop through the mechanisms involving genetics, autoimmunity and viruses. Following our previous reports on the presence of virus-like particles in the NOD mouse (an animal model for type 1 diabetes mellitus), we intended to clone the virus genome and study its expression. NOD mice of 4,6 and 12 weeks of age were examined. Control mice included NON, ICR and B10.G.D.mice. RT-PCR method was used to clone retrovirus cDNA from RNA of the NOD mouse pancreas. PCR cloning was then performed to obtain the retrovirus genome. As a result, we succeeded in cloning the NOD mouse-specific retrovirus (NODV), the sequence of which has not been reported yet. Detailed analysis of NODV revealed following ; NODV is an intrinsic virus incorporated in the NOD mouse genome, its mRNA is expressed only in the pancreas of the NOD mouse, the highest expression of the mRNA is found at 4 weeks of age, i.e.the time when insulitis starts, and LTR of NODV has transcriptional activity in NIT-1 cells derived from NOD mouse beta cells. These results demonstrated the structure and expression of the virus which is thought to be involved in the development of insulitis and diabetes in the NOD mouse. Further analysis of the function of the NODV would disclose the role of viruses in the pathogenesis of type 1 diabetes mellitus.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] M.Namba et al.: "Clinical evaluation of biosynthetic glucagon treatment for recovery from hypoglycemia developed in diabetic patients." Diabetes Res Clin Pract. 19. 133-138 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Asakawa et al.: "Effects of tumor necrosis factor-a on the thyroid peroxidase content,DR antigen expression,and number of microvilli in cultured human thyrocytes." Biomed Res. 14. 5-11 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] N.Itoh et al: "Mononuclear cell infiltration and its relation to the expression of MHC antigens and adhesion molecules in pancreas biopsy specimens from newly diagnosed insulin-dependent diabetes mellitus patients." J Clin Invest. 92. 2313-2322 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Yamagata et al: "Overexpression of HLA class I antigen reduces insulin secretion in pancreatic b cells(RINm5F):evidence for non-immune mechanism." Res Commun Chem Pathol Pharmacol. 81. 299-308 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Oda et al.: "The inhibitory effect of noradrenaline on thyrotrophin-stimulated 3,5,3'-tri-iodothyronine and thyroxine release is mediated through a Ca^<2+>-dependent process in the thyroid gland of the mouse." J Endocrinol. 138. 73-80 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Waguri et al.: "Occurrence of IDDM during interferon therapy for chronic viral hepatitis." Diabetes Res Clin Prac. (in press). (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Hanafusa et al.: "Diabetes in Japan" Elsevier Science Publishers B.V. (in press), (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] N.Itoh, et.al.: "Transthyretin (prealbumin) in the pancreas and sera of newly diagnosed type 1 (insulin-dependent) diabetic patients." J Clin Endocrinol Metab. 74. 1372-1377 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] C.Nakagawa, et.al.: "Retrovirus gag protein p30 in the islets of non-obese diaetic mice : relevance for pathogenesis of diabetes mellitus." Diabetological. 35. 614-618 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M.Namba, et.al.: "Clinical evaluation of biosynthetic glucagon treatment for recovery from hypoglycemia developed in diabetic patients." Diabetes Res Clin Pract. 19. 133-138 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H.Asakawa, et.al.: "Effects of tumor necrosis factor-a on the thyroid peroxidade content, DR antigen expression, and number of microvilli in cultured human thyrocytes." Biomrf Res. 14. 5-11 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] N.Itoh, et.al.: "Mononuclear cell infiltration and its relation to the expression of MHC antigens and adhesion molecules in pancreas biopsy specimens from newly diagnosed IDDM." J Clin Invest. 92. 2313-2322 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Yamagata, et.al.: "Overexpression of HLA class I antigen reduces insulin secretion in pancreatic b cells (RINm5F) : evidence for non-immune mechanism." Res Commun Chem Pathol Pharmacol. 81. 299-308 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T.Hanafusa, et.al.: "The NOD mouse" Diabetes in Japan Elsevier Science Publishers. (in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1995-03-27  

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