1993 Fiscal Year Annual Research Report

ヒトのサイトカインのレセプターの研究:GM-CSFおよびG-CSFを中心として

Research Project

Project/Area Number	04454572
Research Institution	Kyushu University
Principal Investigator	仁保喜之九州大学, 医学部, 教授 (60091287)
Co-Investigator(Kenkyū-buntansha)	久保田晃九州大学, 医学部, 医員原田直樹九州大学, 医学部, 医員池松渉九州大学, 医学部, 医員下田和哉九州大学, 医学部, 医員岡村精一九州大学, 医学部, 助手 (20136435)
Keywords	GM-CSF / G-CSF / レセプター / フローサイトメトリー / モノクローナル抗体 / 刺激伝達 / JAK2 / 燐酸化
Research Abstract	ヒトのサイトカインのレセプターに関してGM-CSFレセプターとG-CSFレセプターを中心として以下の研究を行った. レセプターに対するモノクローナル抗体の作製:GM-CSFレセプターalpha鎖の遺伝子構造から抗原性が強いと考えられる部分のペプチドを合成し、それに対するモノクローナル抗体を作成した.この高力値のモノクローナル抗体はIgM型で,造血細胞でのGM-CSFレセプター分布をフローサイトメトリーで検出可能とした.フローサイトメトリーで使用可能なG-CSFレセプターを認識する抗体も得ている. サイトカイン(CSF)の刺激伝達について:G-CSFは幼若造血前駆細胞を増殖させる働きと,成熟した好中球に働きその機能を変化させるという大きく分けて2つの作用がある.各々の作用の刺激伝達には別のkinaseが関与していると仮説を立てて検討した.すると,分裂能を持つ幼若造血細胞にG-CSFを作用させると,成熟した好中球には認められない分子量約130kdの蛋白が新にtyrosine燐酸化されることを見い出した.さらに,この蛋白はtyrosine燐酸化酵素でもあるJAK2であることを同定した. 膜結合型G-CSFレセプターと可溶性G-CSFレセプターの分布および成熟にともなう変化をRT-PCR法で検出した. さらに,stem cell factorのレセプターであるc-kitやerythropoietinレセプターのフローサイトメトリーによる解析も併せて行った.

Research Products
(12 results)

All Other

All Publications (12 results)

[Publications] Shimoda K.: "Identification of a functional recepter for granulocyte colony-stimulating factor on platelets." Journal of Clinical Investigation. 91. 1310-1313 (1993)
[Publications] Shimoda K.: "Granulocyte colony-stimulating factor and platelet agregation." Lancet. 341. 633- (1993)
[Publications] Shimoda K.: "Human macrophage colony-stimulating factor levels in cerebrospinal fluid." Cytokine. 5. 250-254 (1993)
[Publications] Ikematsu W.: "Phenotypic and immunoglobulin heavy chain gene analysis of B cell chronic lymphocytic leukemia(B-CLL):Expression of myelomonocytic markers in CD5 negative B-CLL." Blood. (印刷中). (1994)
[Publications] Kubota A.: "Augmented production of interleukin 6 by co-culture of human bone marrow adherent cells and human leukemic cells." Biomedicine and Pharamacotherapy. 47. 379-384 (1993)
[Publications] Kubota A.: "The c-kit molecule and the surface immunophenotype of human acute leukemia." Lymphoma Leukemia. (印刷中). (1994)
[Publications] Tajiri J.: "Granulocyte colony-stimulating factor treatment of antithyroid drug-induced granulocytopenia." Archives of Internal Medicine. 153. 509-514 (1993)
[Publications] Takamatsu Y.: "Cytokine production by peripheral blood monocytes and T cells during haematopoietic recovery after intensive chemotherapy." British Journal of Haematology. 83. 21-27 (1993)
[Publications] Tanaka T.: "Differentiation of rat myelomonocytic leukemia cell line(c-WRT-7)cells by rat preadipocyte cellline,REC A16 in vitro." Journal of Clinical and Experimental Oncology. 119. 335-341 (1993)
[Publications] Ohhara N.: "Levels of serum granulocyte colony-stimulating factor in patients with cerebrovascular diseases." Life Science. 53. 939-943 (1993)
[Publications] Hayashi S.: "Sequential expression of lymphokine genes during phytohemagglutinin-stimulated mitogenesis of normal human peripheral mononuclear cells." Biomedicine and Pharmacotherapy. 47. 155-160 (1993)
[Publications] Fukuda Y.: "Predominance of myeloid antigen in CD34 positive peripheral blood stem cells over those in bone marrow,after the administration of granuloyte colony-stimulating factor." European Journal of Haematology. (印刷中). (1994)

1993 Fiscal Year Annual Research Report

ヒトのサイトカインのレセプターの研究:GM-CSFおよびG-CSFを中心として

Principal Investigator

仁保 喜之 九州大学, 医学部, 教授 (60091287)

Research Products

[Publications] Shimoda K.: "Identification of a functional recepter for granulocyte colony-stimulating factor on platelets." Journal of Clinical Investigation. 91. 1310-1313 (1993)

[Publications] Shimoda K.: "Granulocyte colony-stimulating factor and platelet agregation." Lancet. 341. 633- (1993)

[Publications] Shimoda K.: "Human macrophage colony-stimulating factor levels in cerebrospinal fluid." Cytokine. 5. 250-254 (1993)

[Publications] Ikematsu W.: "Phenotypic and immunoglobulin heavy chain gene analysis of B cell chronic lymphocytic leukemia(B-CLL):Expression of myelomonocytic markers in CD5 negative B-CLL." Blood. (印刷中). (1994)

[Publications] Kubota A.: "Augmented production of interleukin 6 by co-culture of human bone marrow adherent cells and human leukemic cells." Biomedicine and Pharamacotherapy. 47. 379-384 (1993)

[Publications] Kubota A.: "The c-kit molecule and the surface immunophenotype of human acute leukemia." Lymphoma Leukemia. (印刷中). (1994)

[Publications] Tajiri J.: "Granulocyte colony-stimulating factor treatment of antithyroid drug-induced granulocytopenia." Archives of Internal Medicine. 153. 509-514 (1993)

[Publications] Takamatsu Y.: "Cytokine production by peripheral blood monocytes and T cells during haematopoietic recovery after intensive chemotherapy." British Journal of Haematology. 83. 21-27 (1993)

[Publications] Tanaka T.: "Differentiation of rat myelomonocytic leukemia cell line(c-WRT-7)cells by rat preadipocyte cellline,REC A16 in vitro." Journal of Clinical and Experimental Oncology. 119. 335-341 (1993)

[Publications] Ohhara N.: "Levels of serum granulocyte colony-stimulating factor in patients with cerebrovascular diseases." Life Science. 53. 939-943 (1993)

[Publications] Hayashi S.: "Sequential expression of lymphokine genes during phytohemagglutinin-stimulated mitogenesis of normal human peripheral mononuclear cells." Biomedicine and Pharmacotherapy. 47. 155-160 (1993)

[Publications] Fukuda Y.: "Predominance of myeloid antigen in CD34 positive peripheral blood stem cells over those in bone marrow,after the administration of granuloyte colony-stimulating factor." European Journal of Haematology. (印刷中). (1994)

仁保喜之九州大学, 医学部, 教授 (60091287)