1994 Fiscal Year Final Research Report Summary
STUDIES ON HUMAN CYTOKINE PECEPTORS WITH SPECIAL REFERENCES TO GM-CSF AND G-CSF
| Project/Area Number |
04454572
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Hematology
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
NIHO Yoshiyuki KYUSHU UNIVERSITY,THE FIRST DEPARTMENT OF INTERNAL MEDICINE,FACULTY OF MEDICINE,PROFESSOR, 医学部, 教授 (60091287)
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| Co-Investigator(Kenkyū-buntansha) |
IWASAKI Hiromi KYUSHU UNIVERSITY,THE FIRST DEPARTMENT OF INTERNAL MEDICINE,FACULTY OF MEDICINE,, 医学部, 医員
KUBOTA Akira KYUSHU UNIVERSITY,THE FIRST DEPARTMENT OF INTERNAL MEDICINE,FACULTY OF MEDICINE,, 医学部, 医員
池松 渉 九州大学, 医学部, 医員
SHIMODA Kozuya KYUSHU UNIVERSITY,THE FIRST DEPARTMENT OF INTERNAL MEDICINE,FACULTY OF MEDICINE,, 医学部, 医員
OKAMURA Seiichi KYUSHU UNIVERSITY,THE FIRST DEPARTMENT OF INTERNAL MEDICINE,FACULTY OF MEDICINE,, 医学部, 講師 (20136435)
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| Project Period (FY) |
1992 – 1994
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| Keywords | G-CSF receptor / platelet / flowcytometry / c-kit / HEL / Jak 1 / Jak 2 / Syk |
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| Research Abstract |
We developed flowcytometric assay system for cytokine receptors (granulocyte colony-stimulating factor receptor : G-CSFR,crythropoietin receptor (Epo-R) using fluorescent dye conjugated ligands. Monoclonal antibodies against GM-CSFR alpha chain and polyclonal antibodies against G-CSFR and GM-CSFR a chain. Various cytokine receptors including c-kit which is receptor for stem cell factor were examined among fresh human samples and leukemia cell lines.
Functional G-CSFRs were found on platelets and mRNA encoding G-CSFR was also detected in platelets and megakaryocytes.
Soluble G-CSFR was also detected with our developed antibodies and ligand binding assay.
Reversible transition of c-kit expression among human erythroleukemia cell line, HEL was firstly found. Some c-kit negative cell changed to positive cells and this implies that c-kit is not merely cell differentiation marker.
Signal transducers after G-CSFR were identified. Using antibodies and anti-sense oligomers against G-CSF dependent leukemic cell lines and the cell line which are induce differentiation with G-CSF,Jak1 and Jak2 tyrosine kinase are related to G-CSF induced cell proliferation and Syk tyrosine kinase is related to G-CSF induced cell differentiation.
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