1993 Fiscal Year Final Research Report Summary
Developmental and molecular genetical analyzes on calpain-calpastatin system at individual and cellular levels
| Project/Area Number |
04454590
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
物質生物化学
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| Research Institution | THE UNIVERSITY OF TOKYO |
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Principal Investigator |
EMORI Yasufumi The University of Tokyo, Graduate School of Science Associate Professor, 大学院・理学系研究科, 助教授 (60160389)
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| Project Period (FY) |
1992 – 1993
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| Keywords | Calpain / Calpastatin / Protease / Calcium / Signal transduction / Drosophila / Cytoskeleton / Melecula biology |
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| Research Abstract |
Calpain is an intracellular cysteine protease, possibly involved in Ca^<2+>-mediated signalling and activated by enhancement of intracellular Ca^<2+> concentration.
First, I have identified two Drosophila genes encoding calpains. Their mRNA expression was studied by Northern blotting analysis and in situ hybridization experiment, which revealed that the two Drosophila calpains belong to conventional calpain family showing ubiquitous distribution.
Next, I prepared a specific antibody against one type of the calpains. Using this antibody, cellular and subcellular localization of the calpain molecule was investigated in the early embryo. The results showed that calpain is localized with actin-related cytoskeletal structure in the very early embryo before cellularization, and in the precleavage furrows just before cellularization. These observations indicate that the Drosophila calpain is involved in univeral cellular functions, especially in the reorganization of actin-related cytoskeleton.
I also studied mode of interaction between calpain and calpastatin, an endogenous inhibitor specific for calpain, by biochemical methods, which provided useful informations for further molecular studies on in vivo functions of the Drosophila calpain-calpastatin system.
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Research Products
(6 results)
