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1994 Fiscal Year Final Research Report Summary

Analysis of Intercellular Interaction in a Process of Neurite Growth

Research Project

Project/Area Number 04454617
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 生物物性学
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

MATSUOKA Hideaki  Tokyo Univ.Agric.Technol., Faculty of Technology, Prof., 工学部, 教授 (10143653)

Project Period (FY) 1992 – 1994
KeywordsPC12h-R / Lone cell culture / Flow culture system / Conditioned medium / Electrical effect / Nerve growth factor / db-cAMP / Neurite growth
Research Abstract

It is known that a neural cell elongates neurites and make a synaptic combination with a target cell. In a process of this neurite growth, some intercellular interaction might be worked between neurons. In order to analyze this intercellular interaction, the present research project was done from 1992 to 1994.
In 1992, using the cultured nerve cells PC12h-R,we developed lone cell culture system which excluded the intercellular interaction absolutely because only one cell was cultivated in a culture well. The usefulness of this system for further research work was shown.
In 1993, a neurite growth initiating activity of various factors (neurotrophic factors, neurotransmitter, hormone, second messenger) was evaluated by using lone cell system. Moreover, a flow culture system which could change the kind and concentration of working factors continuously and satisfied the lone cell culture condition was developed.
In 1994, we analyzed the chemical and physical factors of intercellular interaction by using the developed culture systems. As a chemical factor, some protein, whose molecular weight was 130-170kDa and which induced the neurite growth, was found in the supernatant of the conditioned medium of PC12h-R.It was shown that the stimulation by an alternating current (intensity : >2 muA) also induced the neurite growth. Moreover, in PC12h-R cells, the response of neurites to the repeated actions of NGF or db-cAMP whose action mechanisms were different to neural cells was analyzed.
These results were new knowledge and could be obtained by using our developed culture system. It was concluded that both chemical and physical factors were complicatedly related to the intercellular interaction in the process of the neurite growth.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Yasushi Kazuno: "Use of Lone Cell of PC12h-R as a Sensing Material for Neurite Growth Initiating Activity" J.Intel.Master.Syst.Struct.5. 117-121 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jong-Chul Park: "Adaptation of Aspergillus niger to Several Antifungal Agents." Microbiology. 140. 2409-2414 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hideaki Matsuoka: "Adaptation of Aspergillus niger to Multiple Agents Whose Action Mechanisms are Different." Jpn.J.Med.Mycol. 36. 19 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hyeong-Cheol Yang: "Application of a Microscopic Fluorescence Indicator to the Determination of Antimycotic Activity." J.Antiboct.Antifung.Agents. 23. 3-7 (1995)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hideaki Matsuoka: "Use of Congo Red as a Microscopic Fluorescence Indicator of Hypha Growth." Appl.Microbiol.Biotechnol.41(in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hyeong-Cheol Yang: "Rapid Viability Assessment of Spores of Several Fungi by Ionic Intensified FDA Method." Current Microbiol. 30(in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yasushi Kazuno: "Use of Lone Cell of PC12h-R as a Sensing Material for Neurite Growth Activity." J.Intel.Mater.Syst.Struct.5. 117-121 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jong-Chul Park: "Adaptation of Aspergillus niger to Several Antifungal Agents" Microbiology. 140. 2409-2414 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hideaki Matsuoka: "Adaptation of Aspergillus niger to Multiple Agents Whose Action Mechanisms are Different." Jpn.J.Med.Mycol.36. 19-24 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hyeong-Cheol Yang: "Application of a Microscopic Fluorescence Indicator to the Determination of Antimycotic Activity." J.Antibact.Antifung.Agents. 23. 3-7 (1995)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hideaki Matsuoka: "Use of Congo Red as a Microscopic Fluorescence Indicator of Hypha Growth" Appl.Microbiol.Biotechnol.41 (in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hyeong-Cheol Yang: "Rapid Viability Assessment of Spores of Several Fungi by Ionic Intensified FDA Method." Current Microbiol.30 (in press).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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