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1994 Fiscal Year Final Research Report Summary

DEVELOPMENT OF DNA PROBES FOR THE DIAGNOSIS OF HEMOPARASITE INFECTIONS.

Research Project

Project/Area Number 04556043
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Applied veterinary science
Research InstitutionHOKKAIDO UNIVERSITY

Principal Investigator

SUGIMOTO Chihiro  HOKKAIDO UNIVERSITY,FACULTY OF VETERINARY MEDICINE,ASSOCIATE PROFESSOR, 獣医学部, 助教授 (90231373)

Co-Investigator(Kenkyū-buntansha) OKABE Tatsuji  KYOTO BIKEN CO., SENIOR RESEARCHER, 主任研究員
TAKAHASHI Kiyoshi  COLLEGE OF DAIRY SCIENCE,FACULTY OF DAIRY SCIENCE,PROFESSOR, 酪農学部, 教授 (90048108)
ONUMA Misao  HOKKAIDO UNIVERSITY,FACULTY OF VETERINARY MEDICINE,ASSOCIATE PROFESSOR, 獣医学部, 教授 (70109510)
Project Period (FY) 1992 – 1994
KeywordsDNA probe / PCR / Theileria / Protozoa / Piroplasma
Research Abstract

In order to establish a method of diagnosis for hemoparasite infections, we have developed DNA probes and polymerase chain reaction (PCR) for bovine theileriosis. Two genes of Theileria sergenti encoding p32, a major piroplasm surface protein, and p70, a family of heat shock protein, were cloned and sequenced. The cDNA clone of p32, as well as cloned genomic DNA fragment, was used as a DNA probe. We also developed PCR for bovine theileriosis. These techniques provide sensitive, specific and early diagnosis methods for bovine theileriosis. Genetic diversity of the parasite was revealed by using these DNA probes. Allele-specific PCR was developed to detect and differentiate three allelic forms of p32. By this method, it was revealed that most of fields isolates consisted of a mixture of two to three parasite populations bearing different p32 allele and that population changes might occur during persistent infection and infection cycle between mammalian hosts and vector ticks. The PCR detection system developed in this study will be commercialized.

  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] TANAKA,M.: "Detection of Theileria sergenti infection in cattle by using polymerase chain reaction amplification of parasite specific DNA." Journal of Clinical Microbiology. 31. 2565-2569 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] ALI,S.: "Protein characterization of Babesia equi piroplasms isolated from infected horse erythrocytes." Parasitology Research. 79. 639-643 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] MATSUBA,T.: "Changes in the hybridization patterns of populations of Theileria sergenti during infection." Veterinary Parasitololgy. 47. 215-223 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] MATUBA,T.: "Analysis of mixed parasite populations of Theileria sergenti using cDNA probes encoding a major piroplasm surface protein." Parasitology,. 107. 369-377 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] ZHONG,W.Z.: "Characterization of epitopes on a 32 kDa merozoite surface protein of Theileria sergenti." Parasite Immunology. 15. 113-119 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] KUBOTA,S.: "Molecular cloning and sequencing of hsp 70 cDNA of Theileria sergenti." Molecular and Biochemical Parasitology. 62. 317-318 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] ZHUANG,W.Z.: "Analyses of antigenic diversities of Theileria sergenti piroplasm surface proteins" Journal of Veterinary Medical Science. 56. 469-473 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] WATARAI,S.: "Alteration of ganglioside composition in erythrocytes associated with Theileria sergenti infection." Journal of Veterinary Medical Science. 56. 841-847 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] SUGIMOTO,C.: "Characterization of membrane proteins of Babesia equi piroplasm." Equine Infectious Diseases VII. (発表予定).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] ONOUE,S.: "Prenatal infection with Theileria sergenti in calves." Journal of Protozoology Reserch. (発表予定).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] WATARAI,S.: "Ganglioside as a possible receptor on the erythrocyte for Theileria sergenti." Journal of Veterinary Medical Science. (発表予定).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] ZHUANG,W.Z.: "Antigenic alteration in major piroplasm surface proteins of Theileria sergenti during infection." Veterinary Parasitology.(発表予定).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] TANAKA,M.: "Detection of Theileria sergenti infection in cattle by using polymerase chain reaction amplification of parasite specific DNA." Journal of Clinical Microbiology. 31. 2565-2569 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] ALI,S.: "Protein characterization of Babesia equi piroplasms isolated from infected horse erythrocytes." Parasitology Research. 79. 639-643 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] MATSUBA,T.: "Changes in the hybridization patterns of populations of Theileria sergenti during infection." Veterinary Parasitololgy. 47. 215-223 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] MATSUBA,T.: "Analysis of mixed parasite populations of Theileria sergenti using cDNA probes encoding a major piroplasm surface protein." Parasitology. 107. 369-377 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] ZHUANG,W.Z.: "Characterization of epitopes on a 32 kDa merozoite surface protein of Theileria sergenti." Parasite Immunology. 15. 113-119 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] KUBOTA,S.: "Molecular cloning and sequencing of hsp70 cDNA of Theileria sergenti." Molecular and Biochemical Parasitology. 62. 317-318 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] ZHUANG,W.Z.: "Analyzes of antigenic diversities of Theileria sergenti piroplasm surface proteins." Journal of Veterinary Medical Science. 56. 469-473 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] WATARAI,S.: "Alteration of ganglioside composition in erythrocytes associated with Theileria sergenti infection." Journal of Veterinary Medical Science. 56. 841-847 (1994)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] SUGIMOTO,C.: "Characterization of membyane proteins of Babesia equi piroplasm." Equine Infectious Diseases VII. (In press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] ONOUE,S.: "Prenatal infection with Theileria sergenti in calves." Journal Protozoology Research. in press.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] WATARAI,S.: "Ganglioside as a possible receptor on the erythrocyte for Theileria sergenti." Journal of Veterinary Medical Science. in press.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] ZHUANG,W.Z.: "Antigenic alteration in major piroplasm surface proteins of Theileria sergenti during infection." Veterinary Parasitology. in press.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.KUBOTA: "A genetic analysis of mixed population in Theileria sergenti stocks and isolates using allele-specific polymerase chain reaction." Journal of Veterinary Medical Science. in press.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1996-04-15  

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