1994 Fiscal Year Final Research Report Summary
Visualization of DNA binding protein in situ by using double strand DNA fragment, and an attempt of inhibition of the transcription factors' DNA binding by using double strand DNA fragment.
| Project/Area Number |
04557002
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
神経解剖学
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| Research Institution | Osaka University |
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Principal Investigator |
TOHYAMA Masaya Osaka University, Professor, 医学部, 教授 (40028593)
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| Co-Investigator(Kenkyū-buntansha) |
SHIOSAKA Sadao Nara Institute of Science & Technology, Professor, バイオサイエンス科, 教授 (90127233)
WANAKA Akio Osaka University, Associate Prof., 医学部, 助教授 (90210989)
KIYAMA Hiroshi Osaka University, Associate Prof., 医学部, 助教授 (00192021)
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| Project Period (FY) |
1992 – 1994
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| Keywords | transcription factor / DNA binding protein / nuclear transport signal / gene transfer / liposome / histochemistry / nervous system |
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| Research Abstract |
We investigated following projects
1 : Visualization of DNA binding protein in situ by using double strand DNA fragment.
2 : An attempt for inhibition of transcription factors'DNA binding by using double strand DNA fragment.
For the project3 #1, we have succeeded in establishing the method. We used ^<35>S labeled double strand DNA fragment (Ca.20 base par) which has a AP-1 or CRE motif for the detection of binding protein. The positive signal was observed in cell nucleus.
For the project #2, we developed a method which allowed the efficient transportation of DNA fragment into cell nucleus. This was achieved by conjugating nuclear transport signal peptide from SV40 Large T antigen and wrapping by liposome.
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