1994 Fiscal Year Final Research Report Summary
Development of HLA bound peptitides which have supressive activity.
| Project/Area Number |
04557027
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Immunology
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| Research Institution | KYUSHU UNIVERSITY |
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Principal Investigator |
SASAZUKI Takehiko Kyushu University, Medical Institute of Bioregulation, Department of Genetics, Professor, 生体防御医学研究所, 教授 (50014121)
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| Co-Investigator(Kenkyū-buntansha) |
KAMIKAWAJI Nobuhiro Kyushu University, Medical Institute of Bioregulation, Department of Genetics, A, 生体防御医学研究所, 助手 (90224659)
KIMURA Akinori Kyushu University, Medical Institute of Bioregulation, Department of Genetics, A, 生体防御医学研究所, 助教授 (60161551)
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| Project Period (FY) |
1992 – 1994
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| Keywords | HLA / Antigenic peptide / Immune response / Transgenic mouse / Genetic polymorphism / DNA typing |
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| Research Abstract |
There are many diseases caused by abnormal immune responses. To develop the non stimulatory peptide analog that binds to HLA molecule, we investigated the HLA-peptide interactions. Interaction of HLA-DP9 (DPA1*0201/DPB1*0901) molecule and M protein of serotype 12 (SS95/12) streptococcus has been investigated. Seven antigenic peptides restricted by the HLA-DP9 molecule were identified. Comparison of the amino acid sequences among these peptides revealed HLA-DP9-specific binding motif, which differs from the binding motif to the HLA-DR molecules. In an analysis of T cell responses to synthetic peptides in mice transgenic for HLA-DR51 and -DQ6, we found that DR51 and DQ6 transgenic mice acquired significant T cell response to the peptides. This indicated that HLA transgenic mouse should be a useful tool to examine the function of HLA molecules in vivo. We also developed the DNA typing method using PCR/SSOP analysis for HLA-A,HLA-DR,DQ and DP alleles, and found several new alleles. Using this method we determined several susceptible genes for autoimmune disease.
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