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1993 Fiscal Year Final Research Report Summary

Evaluation of the function and the regeneration ability of the transplanted partial liver using a small amount of hepatocytes obtained by fine needle aspiration biopsy.

Research Project

Project/Area Number 04557057
Research Category

Grant-in-Aid for Developmental Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Digestive surgery
Research InstitutionKYOTO UNIVERSITY

Principal Investigator

TANAKA Koichi  Kyoto University, school of medicine assistant professor, 医学部, 助教授 (20115877)

Co-Investigator(Kenkyū-buntansha) ISHIZAKI Kanji  Radiation Biology Research Center assistant professor, 放射線生物研究センター, 助教授 (70111987)
HONDA Kazuo  School of Medicine assistant, 医学部, 助手 (00209321)
Project Period (FY) 1992 – 1993
Keywordspartial liver transplantation / liver function / aspiration cytology / RT-PCR
Research Abstract

To evaluate the liver function and the regeneration ability after clinical living related donor partial liver transplantation, the method for the determination of the amount of the mRNA of albumin in the hepatocytes contained in the small samples obtained by percutaneous fine needle aspiration biopsy was developed using competitive RT-PCR.The primer for site A was selected from the sequence between exon 4 and exon 5 and the primer for site B was selected from the sequence between exon 11 and exon 12 of the rat albumin gene. The primers introduced with a point mutation, which generate a new restriction site, were synthesized for the site A and the site B.The PCR product from the cDNA obtained by reverse transcriptase using these primers with the point mutation was used as the competitor (internal control), which was recognized by electrophoresis after digestion with the specific endonuclease. Total nucleic acids were extracted from the small amount of the samples of rat liver by Iso Quick^<TM>. The cDNAs were translated from mRNAs using Super Script Preamplification Kit^<TM> and amplified with the genomic DNA and the various amounts of the competitor by PCR.The quantitation of the initial amounts of the mRNA for albumin in the sample was achieved by the determination of the ratio of these products by dencitometry. This method may provide a rapid way to quantitate the amount of the mRNA of albumin in the very small amount of the clinical samples obtained by fine needle aspiration biopsy.

  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] K.Tanaka: "Complication management in living related liver transplantation" American J.Surg. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Tanaka: "Postoperative management based on the Redox theory in living related liver transplantation in children" J.Japanese Society of Pediatr.Surg. 29. 256-263 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Fujita: "Living related liver transplantation for biliary atresia" Clinical Transplantation. (in press).

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S.Uemoto: "Experience with FK506 in living-related-liver-Transplantation" Transplantation. 55. 288-292 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Tanaka: "Liver transplantation in children from living-related donors" Transplant.Proc.25. 1084-1086 (1993)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Tanaka: "Living related liver transplantation" Transplant.Proc.24. 2252-2253 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Ozawa: "Living related douor liver transplantation" KARGER, 250 (1994)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] K.Tanaka: "Complication management in living related liver transplantation" Ame.J.Surg.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Tanaka: "Postoperative management based on the Redox theory in living related liver transplantation in children" J.Jpn.Soc.Ped.Surg.29. 256-263 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Fujita: "Living related liver transplantation for biliary atresia" Clin.Transplant.(in press).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S.Uemoto: "Experience with FK506 in living-related-liver-transplantation" Transplant.55. 288-292 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Tanaka: "Liver transplantation in children from living-related donors" Transplant.Proc.25. 1084-1086 (1993)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Tanaka: "Living related liver transplantation" Transplant.Proc.24. 2252-2253 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] K.Ozawa: "Living related donor liver transplantation" KARGER. 250. (1994)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1995-03-27  

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