1994 Fiscal Year Final Research Report Summary
Development of immunological method for prevention of dental caries using genetic engineering
| Project/Area Number |
04557095
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| Research Category |
Grant-in-Aid for Developmental Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
小児・社会系歯学
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| Research Institution | KYUSHU UNIVERSITY (1993-1994)
国立予防衛生研究所 (1992) |
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Principal Investigator |
KOGA Toshihiko Kyushu University, Faculty of Dentistry, Professor, 歯学部, 教授 (10037541)
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| Co-Investigator(Kenkyū-buntansha) |
NAKANO Yoshio Kyushu University, Faculty of Dentistry, Instructor, 歯学部, 助手 (80253459)
OHO Takahiko Kyushu University, Faculty of Dentistry, Assistant Professor, 歯学部, 講師 (50160940)
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| Project Period (FY) |
1992 – 1994
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| Keywords | Dental caries / Streptococcus mutans / Adherence factor / Protein antigen / Glucosyltransferase / Fusion protein |
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| Research Abstract |
The first step for induction of dental caries by Streptococcus mutans is the adherence of the organism to tooth surfaces. Surface protein antigen (PAc) is considered to participatein the sucrose-independent attachment of the cell to acquired pellicles on tooth surfaces. On the other hand, glucosyltransferase (GTF) is important in the sucrose-dependent adherence of S.mutans.Our epitope mapping using many decapeptides showed that antigenic epitopes were located in the N-terminal A-repeat and middle region of PAc.Moreover, the A-repeat bound to salivary components. We have, therefore, constructed a fusion protein of the fragment corresponding to the A-repeat of PAc and the fragment corresponding to the sucrose-binding site of GTF-I,using an expression plasmid vector pTrc99A.Escherichia coli XL1-blue containing the chimeric plasmid pFPG22 expressed a fusion protein of PAc and GTF-I that reacted with both rabbit anti-PAc serum and antiGTF-I serum. Subcutaneous immunization with this fusion protein induced high titers of antibodies to both adherence factors, and these antibodies inhibited GTF-I, suggesting that the fusion protein may be useful for immunological prevention of dental caries.
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